Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity
In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolo...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2003/151042 |
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| author | Stéphanie Struski Martine Doco‐Fenzy Michael Koehler Ilse Chudoba Francis Levy Linda Masson Nicole Michel Evelyne Ulrich Nadine Gruson Jean Bénard Gérard Potron Pascale Cornillet‐Lefebvre |
| author_facet | Stéphanie Struski Martine Doco‐Fenzy Michael Koehler Ilse Chudoba Francis Levy Linda Masson Nicole Michel Evelyne Ulrich Nadine Gruson Jean Bénard Gérard Potron Pascale Cornillet‐Lefebvre |
| author_sort | Stéphanie Struski |
| collection | DOAJ |
| description | In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex‐FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug‐resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)). Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐3/struski.htm. |
| format | Article |
| id | doaj-art-3d2ef34cd2634cf7bb930c91d0f6e39a |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-3d2ef34cd2634cf7bb930c91d0f6e39a2025-02-03T01:22:13ZengWileyAnalytical Cellular Pathology0921-89121878-36512003-01-0125311512210.1155/2003/151042Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of TumorigenicityStéphanie Struski0Martine Doco‐Fenzy1Michael Koehler2Ilse Chudoba3Francis Levy4Linda Masson5Nicole Michel6Evelyne Ulrich7Nadine Gruson8Jean Bénard9Gérard Potron10Pascale Cornillet‐Lefebvre11Laboratory of Hematology, Robert Debré Hospital and Medical Faculty (UPRES EA 20‐70‐IFR 53 Biomolecules), 51100 Reims, FranceLaboratory of Cytogenetic, Maison Blanche Hospital, 51100 Reims, FranceASI GmbH, 68309 Mannheim, GermanyMetaSystems, 68804 Altlussheim, GermanyPolytec‐PI, Electro‐optics Division, 93694 Pantin, FranceLaboratory of Cytogenetic, Maison Blanche Hospital, 51100 Reims, FranceLaboratory of Cytogenetic, Maison Blanche Hospital, 51100 Reims, FranceLaboratory of Cytogenetic, Maison Blanche Hospital, 51100 Reims, FranceLaboratory of Cytogenetic, Maison Blanche Hospital, 51100 Reims, FranceGustave Roussy Institute, 94800 Villejuif, FranceLaboratory of Hematology, Robert Debré Hospital and Medical Faculty (UPRES EA 20‐70‐IFR 53 Biomolecules), 51100 Reims, FranceLaboratory of Hematology, Robert Debré Hospital and Medical Faculty (UPRES EA 20‐70‐IFR 53 Biomolecules), 51100 Reims, FranceIn order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex‐FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug‐resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)). Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐3/struski.htm.http://dx.doi.org/10.1155/2003/151042 |
| spellingShingle | Stéphanie Struski Martine Doco‐Fenzy Michael Koehler Ilse Chudoba Francis Levy Linda Masson Nicole Michel Evelyne Ulrich Nadine Gruson Jean Bénard Gérard Potron Pascale Cornillet‐Lefebvre Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity Analytical Cellular Pathology |
| title | Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity |
| title_full | Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity |
| title_fullStr | Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity |
| title_full_unstemmed | Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity |
| title_short | Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity |
| title_sort | cytogenetic evolution of human ovarian cell lines associated with chemoresistance and loss of tumorigenicity |
| url | http://dx.doi.org/10.1155/2003/151042 |
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