Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis
Evaluation of skin inflammation biomarkers in canine atopic dermatitis (AD) currently requires skin biopsies. Tape stripping has been shown to be a reliable technique to study biomarkers in the stratum corneum (SC) in humans. The aim of this study was to assess the immune response and identify bioma...
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2024-12-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/1/4 |
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| author | Marion Mosca Nadège Milhau Mélanie Legain Adrien Idée Xavier Langon Didier Pin |
| author_facet | Marion Mosca Nadège Milhau Mélanie Legain Adrien Idée Xavier Langon Didier Pin |
| author_sort | Marion Mosca |
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| description | Evaluation of skin inflammation biomarkers in canine atopic dermatitis (AD) currently requires skin biopsies. Tape stripping has been shown to be a reliable technique to study biomarkers in the stratum corneum (SC) in humans. The aim of this study was to assess the immune response and identify biomarkers in the SC of dogs with canine AD using D-squame<sup>®</sup> as a minimally invasive technique. Eight beagle dogs were epicutaneously sensitized to <i>Dermatophagoides farinae</i> extract after tape stripping on sensitized site (S); twice a week for 49 days. Two sites were determined: lesional site (L) and non-lesional site (NL) on eight dogs affected spontaneously with AD. Adhesive tape strips D-Squame<sup>®</sup> were applied on each site. Skin concentrations of 10 cytokines were analyzed with an ELISA kit. Our results revealed a significant increase of IL-13, IL-4, and TNF-α concentrations in S and L sites. Regarding IFN-γ, its concentration was significantly increased in L skin and increased but not significantly in S sites. All the alarmins were not differentially expressed except IL-33 in the S site. IL-31, IL-1β, and IL-10 were not detectable. D-squame<sup>®</sup> seems to be a suitable technique to extract inflammatory cytokines from the SC of dogs, and IL-13, IL-4, TNF-α, and IFN-γ could be interesting biomarkers of canine AD. |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-3d1e83ec6d40413484b0772de8092b4f2025-01-24T13:51:57ZengMDPI AGVeterinary Sciences2306-73812024-12-01121410.3390/vetsci12010004Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic DermatitisMarion Mosca0Nadège Milhau1Mélanie Legain2Adrien Idée3Xavier Langon4Didier Pin5Interactions Cells Environment, 2016. A104, VetAgro Sup, Dermatology Department, 69280 Marcy L’étoile, FranceInteractions Cells Environment, 2016. A104, VetAgro Sup, Dermatology Department, 69280 Marcy L’étoile, FranceInteractions Cells Environment, 2016. A104, VetAgro Sup, Dermatology Department, 69280 Marcy L’étoile, FranceInteractions Cells Environment, 2016. A104, VetAgro Sup, Dermatology Department, 69280 Marcy L’étoile, FranceResearch and Development Derm Research, Royal Canin, 30470 Aimargues, FranceInteractions Cells Environment, 2016. A104, VetAgro Sup, Dermatology Department, 69280 Marcy L’étoile, FranceEvaluation of skin inflammation biomarkers in canine atopic dermatitis (AD) currently requires skin biopsies. Tape stripping has been shown to be a reliable technique to study biomarkers in the stratum corneum (SC) in humans. The aim of this study was to assess the immune response and identify biomarkers in the SC of dogs with canine AD using D-squame<sup>®</sup> as a minimally invasive technique. Eight beagle dogs were epicutaneously sensitized to <i>Dermatophagoides farinae</i> extract after tape stripping on sensitized site (S); twice a week for 49 days. Two sites were determined: lesional site (L) and non-lesional site (NL) on eight dogs affected spontaneously with AD. Adhesive tape strips D-Squame<sup>®</sup> were applied on each site. Skin concentrations of 10 cytokines were analyzed with an ELISA kit. Our results revealed a significant increase of IL-13, IL-4, and TNF-α concentrations in S and L sites. Regarding IFN-γ, its concentration was significantly increased in L skin and increased but not significantly in S sites. All the alarmins were not differentially expressed except IL-33 in the S site. IL-31, IL-1β, and IL-10 were not detectable. D-squame<sup>®</sup> seems to be a suitable technique to extract inflammatory cytokines from the SC of dogs, and IL-13, IL-4, TNF-α, and IFN-γ could be interesting biomarkers of canine AD.https://www.mdpi.com/2306-7381/12/1/4dogcytokinestape stripsELISAexperimental model |
| spellingShingle | Marion Mosca Nadège Milhau Mélanie Legain Adrien Idée Xavier Langon Didier Pin Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis Veterinary Sciences dog cytokines tape strips ELISA experimental model |
| title | Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis |
| title_full | Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis |
| title_fullStr | Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis |
| title_full_unstemmed | Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis |
| title_short | Use of D-Squame<sup>®</sup> as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis |
| title_sort | use of d squame sup r sup as a minimally invasive technique to evaluate skin immune response biomarkers in canine atopic dermatitis |
| topic | dog cytokines tape strips ELISA experimental model |
| url | https://www.mdpi.com/2306-7381/12/1/4 |
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