Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture
The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L−1 (212 μM) decreased growth rates for about 25% compared...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/401265 |
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| author | Živan Gojkovic Carlos Vílchez Rafael Torronteras Javier Vigara Veronica Gómez-Jacinto Nora Janzer José-Luis Gómez-Ariza Ivana Márová Ines Garbayo |
| author_facet | Živan Gojkovic Carlos Vílchez Rafael Torronteras Javier Vigara Veronica Gómez-Jacinto Nora Janzer José-Luis Gómez-Ariza Ivana Márová Ines Garbayo |
| author_sort | Živan Gojkovic |
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| description | The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L−1 (212 μM) decreased growth rates for about 25% compared to control. A selenate EC50 value of 45 mg·L−1 (238.2 μM) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg−1 of SeMet in 120 h of cultivation. This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se. |
| format | Article |
| id | doaj-art-3d0dc86c98d848b98fdc52c0640a7273 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-3d0dc86c98d848b98fdc52c0640a72732025-02-03T01:32:50ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/401265401265Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch CultureŽivan Gojkovic0Carlos Vílchez1Rafael Torronteras2Javier Vigara3Veronica Gómez-Jacinto4Nora Janzer5José-Luis Gómez-Ariza6Ivana Márová7Ines Garbayo8Algal Biotechnology Group, Department of Chemistry and Material Sciences, Faculty of Experimental Sciences, University of Huelva, Campus el Carmen, Avenida de las Fuerzas Armadas s/n, 21007 Huelva, SpainAlgal Biotechnology Group, Department of Chemistry and Material Sciences, Faculty of Experimental Sciences, University of Huelva, Campus el Carmen, Avenida de las Fuerzas Armadas s/n, 21007 Huelva, SpainDepartment of Environmental Biology and Public Health, University of Huelva, Campus el Carmen, 21007 Huelva, SpainAlgal Biotechnology Group, Department of Chemistry and Material Sciences, Faculty of Experimental Sciences, University of Huelva, Campus el Carmen, Avenida de las Fuerzas Armadas s/n, 21007 Huelva, SpainDepartment of Chemistry and Material Sciences, University of Huelva, Campus el Carmen, 21007 Huelva, SpainUniversity of Applied Science, Giessen, Wiesenstrasse 14, 35390 Giessen, GermanyDepartment of Chemistry and Material Sciences, University of Huelva, Campus el Carmen, 21007 Huelva, SpainDepartment of Food Technology and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 61200 Brno, Czech RepublicAlgal Biotechnology Group, Department of Chemistry and Material Sciences, Faculty of Experimental Sciences, University of Huelva, Campus el Carmen, Avenida de las Fuerzas Armadas s/n, 21007 Huelva, SpainThe aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L−1 (212 μM) decreased growth rates for about 25% compared to control. A selenate EC50 value of 45 mg·L−1 (238.2 μM) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg−1 of SeMet in 120 h of cultivation. This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.http://dx.doi.org/10.1155/2014/401265 |
| spellingShingle | Živan Gojkovic Carlos Vílchez Rafael Torronteras Javier Vigara Veronica Gómez-Jacinto Nora Janzer José-Luis Gómez-Ariza Ivana Márová Ines Garbayo Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture The Scientific World Journal |
| title | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
| title_full | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
| title_fullStr | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
| title_full_unstemmed | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
| title_short | Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture |
| title_sort | effect of selenate on viability and selenomethionine accumulation of chlorella sorokiniana grown in batch culture |
| url | http://dx.doi.org/10.1155/2014/401265 |
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