High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function
Background and Aims. We here assess the effects of PSCs on β-cell function and apoptosis in vivo and in vitro. Materials and Methods. PSCs were transplanted into Wistar and Goto-Kakizaki (GK) rats. Sixteen weeks after transplantation, β-cell function, apoptosis, and islet fibrosis were assessed. I...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/165612 |
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| author | Min Zha Wei Xu Qing Zhai Fengfei Li Bijun Chen Zilin Sun |
| author_facet | Min Zha Wei Xu Qing Zhai Fengfei Li Bijun Chen Zilin Sun |
| author_sort | Min Zha |
| collection | DOAJ |
| description | Background and Aims. We here assess the effects of PSCs on β-cell function and apoptosis in vivo and in vitro. Materials and Methods. PSCs were transplanted into Wistar and Goto-Kakizaki (GK) rats. Sixteen weeks after transplantation, β-cell function, apoptosis, and islet fibrosis were assessed. In vitro the effects of PSCs conditioned medium (PSCs-CM) and/or high concentration of glucose on INS-1 cell function was assessed by measuring insulin secretion, INS-1 cell survival, apoptosis, and endoplasmic reticulum stress (ER stress) associated CHOP expression. Results. PSCs transplantation exacerbated the impaired β-cell function in GK rats, but had no significant effects in Wistar rats. In vitro, PSCs-CM caused impaired INS-1 cell viability and insulin secretion and increased apoptosis, which were more pronounced in the presence of high glucose. Conclusion. Our study demonstrates that PSCs induce β-cell failure in vitro and in vivo. |
| format | Article |
| id | doaj-art-3ca4b49f74a5428a811244578e3f8846 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-3ca4b49f74a5428a811244578e3f88462025-02-03T01:01:49ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/165612165612High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell FunctionMin Zha0Wei Xu1Qing Zhai2Fengfei Li3Bijun Chen4Zilin Sun5Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, Jiangsu 210009, ChinaDepartment of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, Jiangsu 210009, ChinaDepartment of Endocrinology and Genetic Metabolism, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241001, ChinaDepartment of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, Jiangsu 210009, ChinaDepartment of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, Jiangsu 210009, ChinaDepartment of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, Jiangsu 210009, ChinaBackground and Aims. We here assess the effects of PSCs on β-cell function and apoptosis in vivo and in vitro. Materials and Methods. PSCs were transplanted into Wistar and Goto-Kakizaki (GK) rats. Sixteen weeks after transplantation, β-cell function, apoptosis, and islet fibrosis were assessed. In vitro the effects of PSCs conditioned medium (PSCs-CM) and/or high concentration of glucose on INS-1 cell function was assessed by measuring insulin secretion, INS-1 cell survival, apoptosis, and endoplasmic reticulum stress (ER stress) associated CHOP expression. Results. PSCs transplantation exacerbated the impaired β-cell function in GK rats, but had no significant effects in Wistar rats. In vitro, PSCs-CM caused impaired INS-1 cell viability and insulin secretion and increased apoptosis, which were more pronounced in the presence of high glucose. Conclusion. Our study demonstrates that PSCs induce β-cell failure in vitro and in vivo.http://dx.doi.org/10.1155/2014/165612 |
| spellingShingle | Min Zha Wei Xu Qing Zhai Fengfei Li Bijun Chen Zilin Sun High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function International Journal of Endocrinology |
| title | High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function |
| title_full | High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function |
| title_fullStr | High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function |
| title_full_unstemmed | High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function |
| title_short | High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function |
| title_sort | high glucose aggravates the detrimental effects of pancreatic stellate cells on beta cell function |
| url | http://dx.doi.org/10.1155/2014/165612 |
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