Phenolic content and antioxidant activity of Croatian and German honey
Since honey has a therapeutic role in the treatment of many diseases, we investigated the content of phenolic compounds and the antioxidant activity in acacia (Robinia pseudoacacia L.), chestnut (Castanea sativa Mill.) and lime-tree (Tilia spp.) honey originating from Croatia and Germany. Total phen...
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2024-12-01
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Series: | Acta Pharmaceutica |
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Online Access: | https://doi.org/10.2478/acph-2024-0031 |
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author | Bok Valerija Vujčić Šola Ivana Rusak Gordana Budisavljević Alan Nguyen Rosa Ludwig-Müller Jutta Maleš Željan |
author_facet | Bok Valerija Vujčić Šola Ivana Rusak Gordana Budisavljević Alan Nguyen Rosa Ludwig-Müller Jutta Maleš Željan |
author_sort | Bok Valerija Vujčić |
collection | DOAJ |
description | Since honey has a therapeutic role in the treatment of many diseases, we investigated the content of phenolic compounds and the antioxidant activity in acacia (Robinia pseudoacacia L.), chestnut (Castanea sativa Mill.) and lime-tree (Tilia spp.) honey originating from Croatia and Germany. Total phenols, flavonols, and flavanols contents were observed at higher levels in Croatian Castanea honey compared to German Castanea honey. Significant higher values of total flavanols and hydroxycinnamic acids were measured in Croatian Tilia honey compared to German Tilia honey. For Robinia honey, significantly higher values of total phenols and flavonols were observed in almost all Croatian honey samples compared to German honey. Croatian honey samples had higher antioxidant activity compared to German honey samples with most tested methods. The highest total phenols, total flavanols, ABTS, DPPH, and FRAP values were measured in Castanea honey, then in Robinia honey, and the lowest values in Tilia honey samples. With new developed HPLC method, pinobanksin, pinocembrin, and chrysin were identified in the majority of honey samples. Our results imply that both botanical and geographical origin influence the final quality of phenolic compounds and antioxidant activity in honey. A high positive correlation between the results of antioxidant activity and polyphenols was detected. |
format | Article |
id | doaj-art-3c51dec644f04da79a301889ff3302cb |
institution | Kabale University |
issn | 1846-9558 |
language | English |
publishDate | 2024-12-01 |
publisher | Sciendo |
record_format | Article |
series | Acta Pharmaceutica |
spelling | doaj-art-3c51dec644f04da79a301889ff3302cb2025-02-02T15:47:25ZengSciendoActa Pharmaceutica1846-95582024-12-0174467369210.2478/acph-2024-0031Phenolic content and antioxidant activity of Croatian and German honeyBok Valerija Vujčić0Šola Ivana1Rusak Gordana2Budisavljević Alan3Nguyen Rosa4Ludwig-Müller Jutta5Maleš Željan6University of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaTechnische Universität Dresden Faculty of Biology, 01069DresdenGermanyTechnische Universität Dresden Faculty of Biology, 01069DresdenGermanyUniversity of Zagreb Faculty of Pharmacy and BiochemistryDepartment of Pharmaceutical Botany, 10000Zagreb, CroatiaSince honey has a therapeutic role in the treatment of many diseases, we investigated the content of phenolic compounds and the antioxidant activity in acacia (Robinia pseudoacacia L.), chestnut (Castanea sativa Mill.) and lime-tree (Tilia spp.) honey originating from Croatia and Germany. Total phenols, flavonols, and flavanols contents were observed at higher levels in Croatian Castanea honey compared to German Castanea honey. Significant higher values of total flavanols and hydroxycinnamic acids were measured in Croatian Tilia honey compared to German Tilia honey. For Robinia honey, significantly higher values of total phenols and flavonols were observed in almost all Croatian honey samples compared to German honey. Croatian honey samples had higher antioxidant activity compared to German honey samples with most tested methods. The highest total phenols, total flavanols, ABTS, DPPH, and FRAP values were measured in Castanea honey, then in Robinia honey, and the lowest values in Tilia honey samples. With new developed HPLC method, pinobanksin, pinocembrin, and chrysin were identified in the majority of honey samples. Our results imply that both botanical and geographical origin influence the final quality of phenolic compounds and antioxidant activity in honey. A high positive correlation between the results of antioxidant activity and polyphenols was detected.https://doi.org/10.2478/acph-2024-0031antioxidant activityflavonoidsrp-hplccastanea sativa honeyrobinia pseudoacacia honeytilia spp. honey |
spellingShingle | Bok Valerija Vujčić Šola Ivana Rusak Gordana Budisavljević Alan Nguyen Rosa Ludwig-Müller Jutta Maleš Željan Phenolic content and antioxidant activity of Croatian and German honey Acta Pharmaceutica antioxidant activity flavonoids rp-hplc castanea sativa honey robinia pseudoacacia honey tilia spp. honey |
title | Phenolic content and antioxidant activity of Croatian and German honey |
title_full | Phenolic content and antioxidant activity of Croatian and German honey |
title_fullStr | Phenolic content and antioxidant activity of Croatian and German honey |
title_full_unstemmed | Phenolic content and antioxidant activity of Croatian and German honey |
title_short | Phenolic content and antioxidant activity of Croatian and German honey |
title_sort | phenolic content and antioxidant activity of croatian and german honey |
topic | antioxidant activity flavonoids rp-hplc castanea sativa honey robinia pseudoacacia honey tilia spp. honey |
url | https://doi.org/10.2478/acph-2024-0031 |
work_keys_str_mv | AT bokvalerijavujcic phenoliccontentandantioxidantactivityofcroatianandgermanhoney AT solaivana phenoliccontentandantioxidantactivityofcroatianandgermanhoney AT rusakgordana phenoliccontentandantioxidantactivityofcroatianandgermanhoney AT budisavljevicalan phenoliccontentandantioxidantactivityofcroatianandgermanhoney AT nguyenrosa phenoliccontentandantioxidantactivityofcroatianandgermanhoney AT ludwigmullerjutta phenoliccontentandantioxidantactivityofcroatianandgermanhoney AT maleszeljan phenoliccontentandantioxidantactivityofcroatianandgermanhoney |