Phenolic content and antioxidant activity of Croatian and German honey

Since honey has a therapeutic role in the treatment of many diseases, we investigated the content of phenolic compounds and the antioxidant activity in acacia (Robinia pseudoacacia L.), chestnut (Castanea sativa Mill.) and lime-tree (Tilia spp.) honey originating from Croatia and Germany. Total phen...

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Main Authors: Bok Valerija Vujčić, Šola Ivana, Rusak Gordana, Budisavljević Alan, Nguyen Rosa, Ludwig-Müller Jutta, Maleš Željan
Format: Article
Language:English
Published: Sciendo 2024-12-01
Series:Acta Pharmaceutica
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Online Access:https://doi.org/10.2478/acph-2024-0031
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author Bok Valerija Vujčić
Šola Ivana
Rusak Gordana
Budisavljević Alan
Nguyen Rosa
Ludwig-Müller Jutta
Maleš Željan
author_facet Bok Valerija Vujčić
Šola Ivana
Rusak Gordana
Budisavljević Alan
Nguyen Rosa
Ludwig-Müller Jutta
Maleš Željan
author_sort Bok Valerija Vujčić
collection DOAJ
description Since honey has a therapeutic role in the treatment of many diseases, we investigated the content of phenolic compounds and the antioxidant activity in acacia (Robinia pseudoacacia L.), chestnut (Castanea sativa Mill.) and lime-tree (Tilia spp.) honey originating from Croatia and Germany. Total phenols, flavonols, and flavanols contents were observed at higher levels in Croatian Castanea honey compared to German Castanea honey. Significant higher values of total flavanols and hydroxycinnamic acids were measured in Croatian Tilia honey compared to German Tilia honey. For Robinia honey, significantly higher values of total phenols and flavonols were observed in almost all Croatian honey samples compared to German honey. Croatian honey samples had higher antioxidant activity compared to German honey samples with most tested methods. The highest total phenols, total flavanols, ABTS, DPPH, and FRAP values were measured in Castanea honey, then in Robinia honey, and the lowest values in Tilia honey samples. With new developed HPLC method, pinobanksin, pinocembrin, and chrysin were identified in the majority of honey samples. Our results imply that both botanical and geographical origin influence the final quality of phenolic compounds and antioxidant activity in honey. A high positive correlation between the results of antioxidant activity and polyphenols was detected.
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spelling doaj-art-3c51dec644f04da79a301889ff3302cb2025-02-02T15:47:25ZengSciendoActa Pharmaceutica1846-95582024-12-0174467369210.2478/acph-2024-0031Phenolic content and antioxidant activity of Croatian and German honeyBok Valerija Vujčić0Šola Ivana1Rusak Gordana2Budisavljević Alan3Nguyen Rosa4Ludwig-Müller Jutta5Maleš Željan6University of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaUniversity of Zagreb Faculty of Science, Department of Biology (Botany), 10000Zagreb, CroatiaTechnische Universität Dresden Faculty of Biology, 01069DresdenGermanyTechnische Universität Dresden Faculty of Biology, 01069DresdenGermanyUniversity of Zagreb Faculty of Pharmacy and BiochemistryDepartment of Pharmaceutical Botany, 10000Zagreb, CroatiaSince honey has a therapeutic role in the treatment of many diseases, we investigated the content of phenolic compounds and the antioxidant activity in acacia (Robinia pseudoacacia L.), chestnut (Castanea sativa Mill.) and lime-tree (Tilia spp.) honey originating from Croatia and Germany. Total phenols, flavonols, and flavanols contents were observed at higher levels in Croatian Castanea honey compared to German Castanea honey. Significant higher values of total flavanols and hydroxycinnamic acids were measured in Croatian Tilia honey compared to German Tilia honey. For Robinia honey, significantly higher values of total phenols and flavonols were observed in almost all Croatian honey samples compared to German honey. Croatian honey samples had higher antioxidant activity compared to German honey samples with most tested methods. The highest total phenols, total flavanols, ABTS, DPPH, and FRAP values were measured in Castanea honey, then in Robinia honey, and the lowest values in Tilia honey samples. With new developed HPLC method, pinobanksin, pinocembrin, and chrysin were identified in the majority of honey samples. Our results imply that both botanical and geographical origin influence the final quality of phenolic compounds and antioxidant activity in honey. A high positive correlation between the results of antioxidant activity and polyphenols was detected.https://doi.org/10.2478/acph-2024-0031antioxidant activityflavonoidsrp-hplccastanea sativa honeyrobinia pseudoacacia honeytilia spp. honey
spellingShingle Bok Valerija Vujčić
Šola Ivana
Rusak Gordana
Budisavljević Alan
Nguyen Rosa
Ludwig-Müller Jutta
Maleš Željan
Phenolic content and antioxidant activity of Croatian and German honey
Acta Pharmaceutica
antioxidant activity
flavonoids
rp-hplc
castanea sativa honey
robinia pseudoacacia honey
tilia spp. honey
title Phenolic content and antioxidant activity of Croatian and German honey
title_full Phenolic content and antioxidant activity of Croatian and German honey
title_fullStr Phenolic content and antioxidant activity of Croatian and German honey
title_full_unstemmed Phenolic content and antioxidant activity of Croatian and German honey
title_short Phenolic content and antioxidant activity of Croatian and German honey
title_sort phenolic content and antioxidant activity of croatian and german honey
topic antioxidant activity
flavonoids
rp-hplc
castanea sativa honey
robinia pseudoacacia honey
tilia spp. honey
url https://doi.org/10.2478/acph-2024-0031
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AT solaivana phenoliccontentandantioxidantactivityofcroatianandgermanhoney
AT rusakgordana phenoliccontentandantioxidantactivityofcroatianandgermanhoney
AT budisavljevicalan phenoliccontentandantioxidantactivityofcroatianandgermanhoney
AT nguyenrosa phenoliccontentandantioxidantactivityofcroatianandgermanhoney
AT ludwigmullerjutta phenoliccontentandantioxidantactivityofcroatianandgermanhoney
AT maleszeljan phenoliccontentandantioxidantactivityofcroatianandgermanhoney