An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr
<b>Aim:</b> In this case, the anatomical pathways on masticatory muscles of cattle infected rabies virüs have been determined by Real Time PCR (RT-PCR).<p> <b>Materials and Methods:</b> A 1.5-year old male Brown Swiss cattle which was bitten in face by dog was used. The...
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1016 |
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| author | Atilla Yoldaş Ramazan İlgün Nevin Tuzcu Akın Yığın Erdal Özmen |
| author_facet | Atilla Yoldaş Ramazan İlgün Nevin Tuzcu Akın Yığın Erdal Özmen |
| author_sort | Atilla Yoldaş |
| collection | DOAJ |
| description | <b>Aim:</b> In this case, the anatomical pathways on masticatory
muscles of cattle infected rabies virüs have been determined
by Real Time PCR (RT-PCR).<p>
<b>Materials and Methods:</b> A 1.5-year old male Brown Swiss
cattle which was bitten in face by dog was used. The cattle
was died 15 days post exposure to the dog rabies suspected.
The areas where the tissue samples to taken. The origin,
course and branches of the trigeminal nerve were exposed
by standart dissection method. Rabies virus was investigated
on 32 different parts of the cattle's head by means of TaqMan
Probe-Based Real-Time PCR and FAT.<p>
<b>Results:</b> The rabies virus specific nucleic acid was detected
in masseter muscles motor nerves; N. mandibularis branch
of N. trigeminus, N. masticatorius, motor leaf of N. mandibularis,
N. massetericus and Nn. temporalis profunda which are
two extra branches of N. masticatorius, unilaterally ganglion
trigeminale, and pons by Real Time-PCR. However, no rabies
virus has been detected in the samples obtained from other
different parts of the brain.<p>
<b>Conclusion:</b> Contrary to immunohistochemical methods, it
is not possible to trace neuronal connections across synapses
within the brain. RT-PCR method could be helpful to detect
pathways of the neurotropic agents suspected cattle. |
| format | Article |
| id | doaj-art-3c46e9f3d7804dfb9a706f8a4270ae97 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-3c46e9f3d7804dfb9a706f8a4270ae972025-02-03T11:25:40ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532942112151016An anatomical aspect on masseteric muscles in cattle rabies by real-time pcrAtilla Yoldaş0Ramazan İlgün1Nevin Tuzcu2Akın Yığın3Erdal Özmen4Veterinary Research Institute, Adana, TurkeyDepartment of Anatomy, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, TurkeyCumhuriyet University Cutfam, Sivas, TurkeyDepartment of Biology, Faculty of Arts and Sciences, Cukurova University, Adana, TurkeyDepartment of Anatomy, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey<b>Aim:</b> In this case, the anatomical pathways on masticatory muscles of cattle infected rabies virüs have been determined by Real Time PCR (RT-PCR).<p> <b>Materials and Methods:</b> A 1.5-year old male Brown Swiss cattle which was bitten in face by dog was used. The cattle was died 15 days post exposure to the dog rabies suspected. The areas where the tissue samples to taken. The origin, course and branches of the trigeminal nerve were exposed by standart dissection method. Rabies virus was investigated on 32 different parts of the cattle's head by means of TaqMan Probe-Based Real-Time PCR and FAT.<p> <b>Results:</b> The rabies virus specific nucleic acid was detected in masseter muscles motor nerves; N. mandibularis branch of N. trigeminus, N. masticatorius, motor leaf of N. mandibularis, N. massetericus and Nn. temporalis profunda which are two extra branches of N. masticatorius, unilaterally ganglion trigeminale, and pons by Real Time-PCR. However, no rabies virus has been detected in the samples obtained from other different parts of the brain.<p> <b>Conclusion:</b> Contrary to immunohistochemical methods, it is not possible to trace neuronal connections across synapses within the brain. RT-PCR method could be helpful to detect pathways of the neurotropic agents suspected cattle.http://eurasianjvetsci.org/pdf.php3?id=1016masseteric musclerabiesreal time pcr |
| spellingShingle | Atilla Yoldaş Ramazan İlgün Nevin Tuzcu Akın Yığın Erdal Özmen An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr Eurasian Journal of Veterinary Sciences masseteric muscle rabies real time pcr |
| title | An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr |
| title_full | An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr |
| title_fullStr | An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr |
| title_full_unstemmed | An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr |
| title_short | An anatomical aspect on masseteric muscles in cattle rabies by real-time pcr |
| title_sort | anatomical aspect on masseteric muscles in cattle rabies by real time pcr |
| topic | masseteric muscle rabies real time pcr |
| url | http://eurasianjvetsci.org/pdf.php3?id=1016 |
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