EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma
Abstract Background This study aims to investigate EGFR as a prognostic biomarker in oropharyngeal squamous cell carcinoma (OPSCC). Methods OPSCC patients from retrospective (1998–2009) and prospective cohorts (2014–2017) were included. Retrospectively collected tumors were used to construct tissue...
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SAGE Publishing
2019-01-01
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| Series: | Journal of Otolaryngology - Head and Neck Surgery |
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| Online Access: | http://link.springer.com/article/10.1186/s40463-018-0323-6 |
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| author | Shanmugappiriya Sivarajah Morris Kostiuk Cameron Lindsay Lakshmi Puttagunta Daniel A. O’Connell Jeffrey Harris Hadi Seikaly Vincent L. Biron |
| author_facet | Shanmugappiriya Sivarajah Morris Kostiuk Cameron Lindsay Lakshmi Puttagunta Daniel A. O’Connell Jeffrey Harris Hadi Seikaly Vincent L. Biron |
| author_sort | Shanmugappiriya Sivarajah |
| collection | DOAJ |
| description | Abstract Background This study aims to investigate EGFR as a prognostic biomarker in oropharyngeal squamous cell carcinoma (OPSCC). Methods OPSCC patients from retrospective (1998–2009) and prospective cohorts (2014–2017) were included. Retrospectively collected tumors were used to construct tissue microarrays (TMAs), which were stained with EGFR, p16, DAPI and Pan-cytokeratin, and digitally quantified. EGFR, CDKN2A and HPV E6/7 levels from prospectively collected OPSCC was measured by droplet digital PCR (ddPCR). Biomarkers were compared to patient covariates, factors and survival outcomes. Results A total of 249 patients were included retrospectively and 64 patients were enrolled prospectively. p16 status (p < 0.001), smoking above 10 pack years (p = 0.04), smoking above 20 pack years (p < 0.001), total EGFR tumor levels (p = 0.016), and high EGFR within high or low Ki67 tumor nuclear staining (p = 0.03) were found to be significant predictors of 5-year disease specific survival (DSS). A Cox proportional hazard model of DSS showed smoking status and eGFR expression to be dependent of each other on predicting 5-year DSS. ddPCR analysis showed a significant association between smoking status and EGFR levels. Conclusions Total EGFR tumor levels are predictive of 5-year DSS. EGFR levels correlate with. smoking and could be an objective marker for this disease etiology. |
| format | Article |
| id | doaj-art-3c2dffc5747342128cfa0d496afe21e5 |
| institution | Kabale University |
| issn | 1916-0216 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Otolaryngology - Head and Neck Surgery |
| spelling | doaj-art-3c2dffc5747342128cfa0d496afe21e52025-02-03T10:54:12ZengSAGE PublishingJournal of Otolaryngology - Head and Neck Surgery1916-02162019-01-014811810.1186/s40463-018-0323-6EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinomaShanmugappiriya Sivarajah0Morris Kostiuk1Cameron Lindsay2Lakshmi Puttagunta3Daniel A. O’Connell4Jeffrey Harris5Hadi Seikaly6Vincent L. Biron7Division of Otolaryngology-Head & Neck Surgery, University of Alberta HospitalOtolaryngology-Head and Neck Research Laboratory of Alberta, University of AlbertaOtolaryngology-Head and Neck Research Laboratory of Alberta, University of AlbertaDepartment of Laboratory Medicine and Pathology, University of AlbertaDivision of Otolaryngology-Head & Neck Surgery, University of Alberta HospitalDivision of Otolaryngology-Head & Neck Surgery, University of Alberta HospitalDivision of Otolaryngology-Head & Neck Surgery, University of Alberta HospitalDivision of Otolaryngology-Head & Neck Surgery, University of Alberta HospitalAbstract Background This study aims to investigate EGFR as a prognostic biomarker in oropharyngeal squamous cell carcinoma (OPSCC). Methods OPSCC patients from retrospective (1998–2009) and prospective cohorts (2014–2017) were included. Retrospectively collected tumors were used to construct tissue microarrays (TMAs), which were stained with EGFR, p16, DAPI and Pan-cytokeratin, and digitally quantified. EGFR, CDKN2A and HPV E6/7 levels from prospectively collected OPSCC was measured by droplet digital PCR (ddPCR). Biomarkers were compared to patient covariates, factors and survival outcomes. Results A total of 249 patients were included retrospectively and 64 patients were enrolled prospectively. p16 status (p < 0.001), smoking above 10 pack years (p = 0.04), smoking above 20 pack years (p < 0.001), total EGFR tumor levels (p = 0.016), and high EGFR within high or low Ki67 tumor nuclear staining (p = 0.03) were found to be significant predictors of 5-year disease specific survival (DSS). A Cox proportional hazard model of DSS showed smoking status and eGFR expression to be dependent of each other on predicting 5-year DSS. ddPCR analysis showed a significant association between smoking status and EGFR levels. Conclusions Total EGFR tumor levels are predictive of 5-year DSS. EGFR levels correlate with. smoking and could be an objective marker for this disease etiology.http://link.springer.com/article/10.1186/s40463-018-0323-6Oropharyngeal cancerHPVSurvivalSmokingEGFR |
| spellingShingle | Shanmugappiriya Sivarajah Morris Kostiuk Cameron Lindsay Lakshmi Puttagunta Daniel A. O’Connell Jeffrey Harris Hadi Seikaly Vincent L. Biron EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma Journal of Otolaryngology - Head and Neck Surgery Oropharyngeal cancer HPV Survival Smoking EGFR |
| title | EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma |
| title_full | EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma |
| title_fullStr | EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma |
| title_full_unstemmed | EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma |
| title_short | EGFR as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma |
| title_sort | egfr as a biomarker of smoking status and survival in oropharyngeal squamous cell carcinoma |
| topic | Oropharyngeal cancer HPV Survival Smoking EGFR |
| url | http://link.springer.com/article/10.1186/s40463-018-0323-6 |
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