Targeted sequencing analysis pipeline for species identification of human pathogenic fungi using long-read nanopore sequencing

Targeted sequencing analysis pipeline for species identification of human pathogenic fungi using long-read nanopore sequencing

Abstract Among molecular-based techniques for fungal identification, Sanger sequencing of the primary universal fungal DNA barcode, the internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), is commonly used in clinical routine laboratories due to its simplicity, universality, efficacy, and af...

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Main Authors: Nattapong Langsiri, Navaporn Worasilchai, Laszlo Irinyi, Piroon Jenjaroenpun, Thidathip Wongsurawat, Janet Jennifer Luangsa-ard, Wieland Meyer, Ariya Chindamporn
Format: Article
Language:English
Published: BMC 2023-09-01
Series:IMA Fungus
Subjects:
Internal transcribed spacer (ITS)
Targeted long-read sequencing
Nanopore technology
Fungal identification
Online Access:https://doi.org/10.1186/s43008-023-00125-6
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