Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study
Since the beginning of their production and use, fossil fuels have affected ecosystems, causing significant damage to their biodiversity. Bacterial bioremediation can provide solutions to this environmental problem. In this study, the new species Isoptericola peretonis sp. nov. 4D.3T has been charac...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1471121/full |
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| author | Àngela Vidal-Verdú Adriel Latorre-Pérez Javier Pascual Ruth Mañes-Collado Aitana Nevot-Terraes Manuel Porcar Manuel Porcar |
| author_facet | Àngela Vidal-Verdú Adriel Latorre-Pérez Javier Pascual Ruth Mañes-Collado Aitana Nevot-Terraes Manuel Porcar Manuel Porcar |
| author_sort | Àngela Vidal-Verdú |
| collection | DOAJ |
| description | Since the beginning of their production and use, fossil fuels have affected ecosystems, causing significant damage to their biodiversity. Bacterial bioremediation can provide solutions to this environmental problem. In this study, the new species Isoptericola peretonis sp. nov. 4D.3T has been characterized and compared to other closely related species in terms of hydrocarbon degradation and biosurfactant production by in vitro and in silico analyses. Biosurfactants play an important role in microbial hydrocarbon degradation by emulsifying hydrocarbons and making them accessible to the microbial degradation machinery. The tests performed showed positive results to a greater or lesser degree for all strains. In the synthesis of biosurfactants, all the strains tested showed biosurfactant activity in three complementary assays (CTAB, hemolysis and E24%) and rhamnolipid synthesis genes have been predicted in silico in the majority of Isoptericola strains. Regarding hydrocarbon degradation, all the Isoptericola strains analyzed presented putative genes responsible for the aerobic and anaerobic degradation of aromatic and alkane hydrocarbons. Overall, our results highlight the metabolic diversity and the biochemical robustness of the Isoptericola genus which is proposed to be of interest in the field of hydrocarbon bioremediation. |
| format | Article |
| id | doaj-art-3bbbc418db10442aba5387ed4c9037bf |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-3bbbc418db10442aba5387ed4c9037bf2025-02-05T23:03:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.14711211471121Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative studyÀngela Vidal-Verdú0Adriel Latorre-Pérez1Javier Pascual2Ruth Mañes-Collado3Aitana Nevot-Terraes4Manuel Porcar5Manuel Porcar6Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Paterna, SpainDarwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Paterna, SpainDarwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Paterna, SpainInstitute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Paterna, SpainInstitute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Paterna, SpainInstitute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Paterna, SpainDarwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Paterna, SpainSince the beginning of their production and use, fossil fuels have affected ecosystems, causing significant damage to their biodiversity. Bacterial bioremediation can provide solutions to this environmental problem. In this study, the new species Isoptericola peretonis sp. nov. 4D.3T has been characterized and compared to other closely related species in terms of hydrocarbon degradation and biosurfactant production by in vitro and in silico analyses. Biosurfactants play an important role in microbial hydrocarbon degradation by emulsifying hydrocarbons and making them accessible to the microbial degradation machinery. The tests performed showed positive results to a greater or lesser degree for all strains. In the synthesis of biosurfactants, all the strains tested showed biosurfactant activity in three complementary assays (CTAB, hemolysis and E24%) and rhamnolipid synthesis genes have been predicted in silico in the majority of Isoptericola strains. Regarding hydrocarbon degradation, all the Isoptericola strains analyzed presented putative genes responsible for the aerobic and anaerobic degradation of aromatic and alkane hydrocarbons. Overall, our results highlight the metabolic diversity and the biochemical robustness of the Isoptericola genus which is proposed to be of interest in the field of hydrocarbon bioremediation.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1471121/fullIsoptericolanew specieshydrocarbon degradationbiosurfactantsbioremediation |
| spellingShingle | Àngela Vidal-Verdú Adriel Latorre-Pérez Javier Pascual Ruth Mañes-Collado Aitana Nevot-Terraes Manuel Porcar Manuel Porcar Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study Frontiers in Microbiology Isoptericola new species hydrocarbon degradation biosurfactants bioremediation |
| title | Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study |
| title_full | Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study |
| title_fullStr | Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study |
| title_full_unstemmed | Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study |
| title_short | Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study |
| title_sort | assessing hydrocarbon degradation capacity of isoptericola peretonis sp nov and related species a comparative study |
| topic | Isoptericola new species hydrocarbon degradation biosurfactants bioremediation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1471121/full |
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