Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, India
Control and preventive measures for gonococcal infections are based on precise epidemiological characteristics of N. gonorrhoeae isolates. In the present study the potential utility of opa-typing and ribotyping for molecular epidemiological study of consecutive gonococcal strains was determined. Si...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2009/934823 |
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| author | Pejvak Khaki Preena Bhalla Ahmad Mir Fayaz Sohiela Moradi Bidhendi Majid Esmailzadeh Pawan Sharma |
| author_facet | Pejvak Khaki Preena Bhalla Ahmad Mir Fayaz Sohiela Moradi Bidhendi Majid Esmailzadeh Pawan Sharma |
| author_sort | Pejvak Khaki |
| collection | DOAJ |
| description | Control and preventive measures for gonococcal infections are based on precise epidemiological characteristics of N. gonorrhoeae isolates. In the present study the potential utility of opa-typing and ribotyping for molecular epidemiological study of consecutive gonococcal strains was determined. Sixty gonococcal isolates were subjected to ribotyping with two restriction enzymes, AvaII and HincII, and opa-typing with TaqI and HpaII for epidemiological characterization of gonococcal population. Ribotyping with AvaII yielded 6 ribotype patterns while twelve RFLP patterns were observed with HincII. Opa-typing of the 60 isolates revealed a total 54 opa-types, which 48 were unique and 6 formed clusters. Fifty-two opa-types were observed with TaqI-digested PCR product while opa-typing with HpaII demonstrated 54 opa-types. The opa-types from isolates that were epidemiologically unrelated were distinct, whereas those from the sexual contacts were identical. The results showed that opa-typing is highly useful for characterizing gonococcal strains from sexual contacts and has more discriminatory than ribotyping that could differentiate between gonococci of the same ribotype. The technique even with a single restriction enzyme has a high level of discrimination (99.9%) between epidemiologically unrelated isolates. In conclusion, the molecular methods such as opa-typing and ribotyping can be used for epidemiological characterization of gonococcal strains. |
| format | Article |
| id | doaj-art-3ba57f87a36f46d6bc35281c14377a86 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-3ba57f87a36f46d6bc35281c14377a862025-02-03T05:49:33ZengWileyInternational Journal of Microbiology1687-918X1687-91982009-01-01200910.1155/2009/934823934823Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, IndiaPejvak Khaki0Preena Bhalla1Ahmad Mir Fayaz2Sohiela Moradi Bidhendi3Majid Esmailzadeh4Pawan Sharma5Microbiology Department, Razi Vaccine and Serum Research Institute, Karaj, IranMicrobiology Department, Maulana Azad Medical College, New Delhi, IndiaInternational Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaMicrobiology Department, Razi Vaccine and Serum Research Institute, Karaj, IranMicrobiology Department, Razi Vaccine and Serum Research Institute, Karaj, IranInternational Centre for Genetic Engineering and Biotechnology, New Delhi, IndiaControl and preventive measures for gonococcal infections are based on precise epidemiological characteristics of N. gonorrhoeae isolates. In the present study the potential utility of opa-typing and ribotyping for molecular epidemiological study of consecutive gonococcal strains was determined. Sixty gonococcal isolates were subjected to ribotyping with two restriction enzymes, AvaII and HincII, and opa-typing with TaqI and HpaII for epidemiological characterization of gonococcal population. Ribotyping with AvaII yielded 6 ribotype patterns while twelve RFLP patterns were observed with HincII. Opa-typing of the 60 isolates revealed a total 54 opa-types, which 48 were unique and 6 formed clusters. Fifty-two opa-types were observed with TaqI-digested PCR product while opa-typing with HpaII demonstrated 54 opa-types. The opa-types from isolates that were epidemiologically unrelated were distinct, whereas those from the sexual contacts were identical. The results showed that opa-typing is highly useful for characterizing gonococcal strains from sexual contacts and has more discriminatory than ribotyping that could differentiate between gonococci of the same ribotype. The technique even with a single restriction enzyme has a high level of discrimination (99.9%) between epidemiologically unrelated isolates. In conclusion, the molecular methods such as opa-typing and ribotyping can be used for epidemiological characterization of gonococcal strains.http://dx.doi.org/10.1155/2009/934823 |
| spellingShingle | Pejvak Khaki Preena Bhalla Ahmad Mir Fayaz Sohiela Moradi Bidhendi Majid Esmailzadeh Pawan Sharma Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, India International Journal of Microbiology |
| title | Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, India |
| title_full | Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, India |
| title_fullStr | Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, India |
| title_full_unstemmed | Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, India |
| title_short | Molecular Typing of Neisseria gonorrhoeae Isolates by Opa-Typing and Ribotyping in New Delhi, India |
| title_sort | molecular typing of neisseria gonorrhoeae isolates by opa typing and ribotyping in new delhi india |
| url | http://dx.doi.org/10.1155/2009/934823 |
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