Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress
The bHLH gene family plays a critical role in regulating internal responses in plants. Although the pharmacological properties of Scutellaria baicalensis have been extensively studied, its bHLH gene family remains poorly investigated. In this study, 142 SbbHLH genes were identified using the complet...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1506805/full |
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| author | Yingxin Sun Beier Wang Lichao Zhang Xiaohan Zheng Peng Xu Meng Zhang Meiguang Han Peng Di Mei Han Lin Cheng Limin Yang |
| author_facet | Yingxin Sun Beier Wang Lichao Zhang Xiaohan Zheng Peng Xu Meng Zhang Meiguang Han Peng Di Mei Han Lin Cheng Limin Yang |
| author_sort | Yingxin Sun |
| collection | DOAJ |
| description | The bHLH gene family plays a critical role in regulating internal responses in plants. Although the pharmacological properties of Scutellaria baicalensis have been extensively studied, its bHLH gene family remains poorly investigated. In this study, 142 SbbHLH genes were identified using the complete genome data of S. baicalensis. Phylogenetic and conserved motif analyses were performed. Gene duplication events were analyzed, and cis-element analysis was conducted to explore regulatory factors. The expression patterns of these genes in different tissues and under drought stress were investigated using transcriptome data and qRT-PCR analysis. Phylogenetic and conserved motif analyses revealed that the gene structures within each SbbHLH clade are relatively conserved. Gene duplication analysis identified 29 duplication events in the SbbHLH gene family, most of which involved gene pairs under purifying selection. Cis-element analysis revealed that these genes are regulated by various environmental and hormonal factors. Transcriptomic data and qRT-PCR results demonstrated tissue-specific expression patterns for the 142 SbbHLH genes. Additionally, bHLH genes potentially involved in baicalin biosynthesis were identified under drought stress. The findings suggest that under drought stress, SbbHLH74, SbbHLH98, and SbbHLH142 are regulated by a network centered on SbbHLH53, which enhances baicalin biosynthesis. In conclusion, this study provides a comprehensive analysis of the bHLH gene family in S. baicalensis and identifies 4 potential SbbHLH genes involved in regulating baicalin biosynthesis under drought stress. |
| format | Article |
| id | doaj-art-3b81ba33b93f4ac59992635c64b3f5e2 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-3b81ba33b93f4ac59992635c64b3f5e22025-01-27T06:40:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15068051506805Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stressYingxin Sun0Beier Wang1Lichao Zhang2Xiaohan Zheng3Peng Xu4Meng Zhang5Meiguang Han6Peng Di7Mei Han8Lin Cheng9Limin Yang10Cultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaTechnology Service Center on Ecological Planting of Chinese Herbal Medicine in Chengde, Chengde, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaTechnology Service Center on Ecological Planting of Chinese Herbal Medicine in Chengde, Chengde, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaThe bHLH gene family plays a critical role in regulating internal responses in plants. Although the pharmacological properties of Scutellaria baicalensis have been extensively studied, its bHLH gene family remains poorly investigated. In this study, 142 SbbHLH genes were identified using the complete genome data of S. baicalensis. Phylogenetic and conserved motif analyses were performed. Gene duplication events were analyzed, and cis-element analysis was conducted to explore regulatory factors. The expression patterns of these genes in different tissues and under drought stress were investigated using transcriptome data and qRT-PCR analysis. Phylogenetic and conserved motif analyses revealed that the gene structures within each SbbHLH clade are relatively conserved. Gene duplication analysis identified 29 duplication events in the SbbHLH gene family, most of which involved gene pairs under purifying selection. Cis-element analysis revealed that these genes are regulated by various environmental and hormonal factors. Transcriptomic data and qRT-PCR results demonstrated tissue-specific expression patterns for the 142 SbbHLH genes. Additionally, bHLH genes potentially involved in baicalin biosynthesis were identified under drought stress. The findings suggest that under drought stress, SbbHLH74, SbbHLH98, and SbbHLH142 are regulated by a network centered on SbbHLH53, which enhances baicalin biosynthesis. In conclusion, this study provides a comprehensive analysis of the bHLH gene family in S. baicalensis and identifies 4 potential SbbHLH genes involved in regulating baicalin biosynthesis under drought stress.https://www.frontiersin.org/articles/10.3389/fpls.2024.1506805/fullScutellaria baicalensisbHLH gene familyexpression patternbaicalin biosynthesisdrought stress |
| spellingShingle | Yingxin Sun Beier Wang Lichao Zhang Xiaohan Zheng Peng Xu Meng Zhang Meiguang Han Peng Di Mei Han Lin Cheng Limin Yang Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress Frontiers in Plant Science Scutellaria baicalensis bHLH gene family expression pattern baicalin biosynthesis drought stress |
| title | Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress |
| title_full | Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress |
| title_fullStr | Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress |
| title_full_unstemmed | Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress |
| title_short | Genome-wide identification of the bHLH gene family in Scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress |
| title_sort | genome wide identification of the bhlh gene family in scutellaria baicalensis and their relationship with baicalin biosynthesis under drought stress |
| topic | Scutellaria baicalensis bHLH gene family expression pattern baicalin biosynthesis drought stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1506805/full |
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