Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36
This study aimed to explore the mechanism by which total flavones of Abelmoschl manihot reduce intracellular lipid deposition in HK-2 cells (Human renal cortex proximal tubule epithelial cells) and thus reduce cell apoptosis. Palmitic acid was employed to induce intracellular lipid deposition. The...
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Bangladesh Pharmacological Society
2024-12-01
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| Series: | Bangladesh Journal of Pharmacology |
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| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/77820 |
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| author | Xiaofang Wang Chenquan Tang Yi Jiang Yi Xue Enchao Zhou |
| author_facet | Xiaofang Wang Chenquan Tang Yi Jiang Yi Xue Enchao Zhou |
| author_sort | Xiaofang Wang |
| collection | DOAJ |
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This study aimed to explore the mechanism by which total flavones of Abelmoschl manihot reduce intracellular lipid deposition in HK-2 cells (Human renal cortex proximal tubule epithelial cells) and thus reduce cell apoptosis. Palmitic acid was employed to induce intracellular lipid deposition. The cell proliferation activity was detected by the CCK-8 assay. The cell death status was evaluated by flow cytometry. The intracellular lipid deposition was observed by oil red O and BODIPY probe staining. The expression level of CD36 in HK-2 cells was determined by western blot. The results indicated that total flavones of A. manihot could inhibit the expression of CD36 in HK-2 cells in a dose-dependent manner and reduce lipid deposition. Consequently, total flavones of A. manihot protect HK-2 cells by reducing intracellular lipid deposition by inhibiting CD36-mediated fatty acid uptake.
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| format | Article |
| id | doaj-art-3b230d4c583a4e258952dc3be9a07596 |
| institution | Kabale University |
| issn | 1991-0088 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj-art-3b230d4c583a4e258952dc3be9a075962025-02-02T13:09:57ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882024-12-0119310.3329/bjp.v19i3.77820Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36Xiaofang Wang0Chenquan Tang1Yi Jiang2https://orcid.org/0000-0001-8960-2056Yi Xue3https://orcid.org/0000-0002-3657-1214Enchao Zhou4https://orcid.org/0000-0003-2569-5657No. 1 Clinical Medical College, Nanjing University of Chinese Medicine (Jiangsu Province Hospital of Chinese Medicine), Nanjing 210000, China.Department of Nephrology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215100, China.Department of Nephrology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215100, China.Department of Nephrology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215100, China.No. 1 Clinical Medical College, Nanjing University of Chinese Medicine (Jiangsu Province Hospital of Chinese Medicine), Nanjing 210000, China.. This study aimed to explore the mechanism by which total flavones of Abelmoschl manihot reduce intracellular lipid deposition in HK-2 cells (Human renal cortex proximal tubule epithelial cells) and thus reduce cell apoptosis. Palmitic acid was employed to induce intracellular lipid deposition. The cell proliferation activity was detected by the CCK-8 assay. The cell death status was evaluated by flow cytometry. The intracellular lipid deposition was observed by oil red O and BODIPY probe staining. The expression level of CD36 in HK-2 cells was determined by western blot. The results indicated that total flavones of A. manihot could inhibit the expression of CD36 in HK-2 cells in a dose-dependent manner and reduce lipid deposition. Consequently, total flavones of A. manihot protect HK-2 cells by reducing intracellular lipid deposition by inhibiting CD36-mediated fatty acid uptake. https://www.banglajol.info/index.php/BJP/article/view/77820Abelmoschus manihot CD36Fatty acid uptake Flavonoid HK-2 cellLipid deposition |
| spellingShingle | Xiaofang Wang Chenquan Tang Yi Jiang Yi Xue Enchao Zhou Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36 Bangladesh Journal of Pharmacology Abelmoschus manihot CD36 Fatty acid uptake Flavonoid HK-2 cell Lipid deposition |
| title | Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36 |
| title_full | Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36 |
| title_fullStr | Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36 |
| title_full_unstemmed | Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36 |
| title_short | Total flavonoids of Abelmoschus manihot ameliorate lipid deposition in HK-2 cells by inhibiting fatty acid uptake mediated by CD36 |
| title_sort | total flavonoids of abelmoschus manihot ameliorate lipid deposition in hk 2 cells by inhibiting fatty acid uptake mediated by cd36 |
| topic | Abelmoschus manihot CD36 Fatty acid uptake Flavonoid HK-2 cell Lipid deposition |
| url | https://www.banglajol.info/index.php/BJP/article/view/77820 |
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