Development and application of sugarcane yellow leaf virus vectors in sugarcane
Abstract The aphid-transmitted polerovirus, sugarcane yellow leaf virus (SCYLV), is one of the most widespread sugarcane viruses and causes huge economic losses to the global sugarcane production. Molecular studies and disease resistance breeding against SCYLV are impeded both by the lack of infecti...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Phytopathology Research |
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| Online Access: | https://doi.org/10.1186/s42483-024-00295-0 |
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| author | Yuteng Yin Pingzhi Zhao Yanwei Sun Tian Han Mengrong Wang Meixin Yan Jianming Wu Hui Zhou Jian Ye |
| author_facet | Yuteng Yin Pingzhi Zhao Yanwei Sun Tian Han Mengrong Wang Meixin Yan Jianming Wu Hui Zhou Jian Ye |
| author_sort | Yuteng Yin |
| collection | DOAJ |
| description | Abstract The aphid-transmitted polerovirus, sugarcane yellow leaf virus (SCYLV), is one of the most widespread sugarcane viruses and causes huge economic losses to the global sugarcane production. Molecular studies and disease resistance breeding against SCYLV are impeded both by the lack of infectious clones and comprehensive knowledge of this virus. In this study, we present the development of the first infectious cDNA clone of SCYLV, which could efficiently infect Nicotiana benthamiana, Arabidopsis thaliana and sugarcane plants. SCYLV-based recombinant heterologous EGFP protein vector was also developed. Using this system, we identified the green peach aphid, Myzus persicae, as a new compatible transmission vector and recognized LC05-136 and GT10-2118 as more resistant cultivars of SCYLV than Badila. This system provides an indispensable resource for both SCYLV biological research and sugarcane breeding efforts. |
| format | Article |
| id | doaj-art-3b064e76c88049da86bf57fd5707c505 |
| institution | Kabale University |
| issn | 2524-4167 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Phytopathology Research |
| spelling | doaj-art-3b064e76c88049da86bf57fd5707c5052025-01-26T12:17:49ZengBMCPhytopathology Research2524-41672025-01-01711910.1186/s42483-024-00295-0Development and application of sugarcane yellow leaf virus vectors in sugarcaneYuteng Yin0Pingzhi Zhao1Yanwei Sun2Tian Han3Mengrong Wang4Meixin Yan5Jianming Wu6Hui Zhou7Jian Ye8State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesSugarcane Research Institute, Guangxi Academy of Agricultural SciencesSugarcane Research Institute, Guangxi Academy of Agricultural SciencesSugarcane Research Institute, Guangxi Academy of Agricultural SciencesState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesAbstract The aphid-transmitted polerovirus, sugarcane yellow leaf virus (SCYLV), is one of the most widespread sugarcane viruses and causes huge economic losses to the global sugarcane production. Molecular studies and disease resistance breeding against SCYLV are impeded both by the lack of infectious clones and comprehensive knowledge of this virus. In this study, we present the development of the first infectious cDNA clone of SCYLV, which could efficiently infect Nicotiana benthamiana, Arabidopsis thaliana and sugarcane plants. SCYLV-based recombinant heterologous EGFP protein vector was also developed. Using this system, we identified the green peach aphid, Myzus persicae, as a new compatible transmission vector and recognized LC05-136 and GT10-2118 as more resistant cultivars of SCYLV than Badila. This system provides an indispensable resource for both SCYLV biological research and sugarcane breeding efforts.https://doi.org/10.1186/s42483-024-00295-0Sugarcane yellow leaf virusSugarcaneAphidInfectious cloneExpression vector |
| spellingShingle | Yuteng Yin Pingzhi Zhao Yanwei Sun Tian Han Mengrong Wang Meixin Yan Jianming Wu Hui Zhou Jian Ye Development and application of sugarcane yellow leaf virus vectors in sugarcane Phytopathology Research Sugarcane yellow leaf virus Sugarcane Aphid Infectious clone Expression vector |
| title | Development and application of sugarcane yellow leaf virus vectors in sugarcane |
| title_full | Development and application of sugarcane yellow leaf virus vectors in sugarcane |
| title_fullStr | Development and application of sugarcane yellow leaf virus vectors in sugarcane |
| title_full_unstemmed | Development and application of sugarcane yellow leaf virus vectors in sugarcane |
| title_short | Development and application of sugarcane yellow leaf virus vectors in sugarcane |
| title_sort | development and application of sugarcane yellow leaf virus vectors in sugarcane |
| topic | Sugarcane yellow leaf virus Sugarcane Aphid Infectious clone Expression vector |
| url | https://doi.org/10.1186/s42483-024-00295-0 |
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