A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i>
<i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> are two species belonging to the Plusiinae subfamily within the Noctuidae family. Due to their morphological similarity, the identification of their larvae is difficult and time-consuming. A rapid and accurate identifica...
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MDPI AG
2024-12-01
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| author | Guilherme A. Gotardi Natália R. F. Batista Tamylin Kaori Ishizuka Luiz H. Marques Mário H. Dal Pogetto Amit Sethi Mark L. Dahmer Timothy Nowatzki |
| author_facet | Guilherme A. Gotardi Natália R. F. Batista Tamylin Kaori Ishizuka Luiz H. Marques Mário H. Dal Pogetto Amit Sethi Mark L. Dahmer Timothy Nowatzki |
| author_sort | Guilherme A. Gotardi |
| collection | DOAJ |
| description | <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> are two species belonging to the Plusiinae subfamily within the Noctuidae family. Due to their morphological similarity, the identification of their larvae is difficult and time-consuming. A rapid and accurate identification of these two species is essential for their management as these species exhibit differential susceptibilities to insecticides and crops engineered to express <i>Bacillus thuringiensis</i> (<i>Bt</i>) proteins, and a molecular tool can easily provide this differentiation. Currently, molecular analysis can identify these species through genetic sequencing, an expensive and time-consuming process. In our study, after sequencing part of the mtDNA cytochrome c oxidase I (<i>COI</i>) gene and based on the differences found in the gene of each species, a set of species-specific primers was developed: one reverse primer common to both species and two forward primers, specific to each species, amplifying fragments of 199 base pairs (bp) for <i>C. includens</i> and 299 bp for <i>R. nu</i>. Our results indicate that the primers were specific for these species, enabling the identification of individuals directly through agarose gel. The new methodology proved to be accurate, rapid, and reliable for the correct identification of these two species of loopers. |
| format | Article |
| id | doaj-art-3ac6eb9dba9b4bcc9fc5ae51bf6c5e65 |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-3ac6eb9dba9b4bcc9fc5ae51bf6c5e652025-08-20T02:53:19ZengMDPI AGInsects2075-44502024-12-01151296910.3390/insects15120969A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i>Guilherme A. Gotardi0Natália R. F. Batista1Tamylin Kaori Ishizuka2Luiz H. Marques3Mário H. Dal Pogetto4Amit Sethi5Mark L. Dahmer6Timothy Nowatzki7Corteva Agriscience, Rodovia SP 147 Km 71, Mogi Mirim 13801-540, SP, BrazilCorteva Agriscience, Rodovia SP 147 Km 71, Mogi Mirim 13801-540, SP, BrazilCorteva Agriscience, Rodovia SP 147 Km 71, Mogi Mirim 13801-540, SP, BrazilCorteva Agriscience, Rodovia SP 147 Km 71, Mogi Mirim 13801-540, SP, BrazilCorteva Agriscience, 9330 Zionsville, Indianapolis, IN 46268, USACorteva Agriscience, 7100 South Dr, Johnston, IA 50131, USACorteva Agriscience, 7100 South Dr, Johnston, IA 50131, USACorteva Agriscience, 7100 South Dr, Johnston, IA 50131, USA<i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> are two species belonging to the Plusiinae subfamily within the Noctuidae family. Due to their morphological similarity, the identification of their larvae is difficult and time-consuming. A rapid and accurate identification of these two species is essential for their management as these species exhibit differential susceptibilities to insecticides and crops engineered to express <i>Bacillus thuringiensis</i> (<i>Bt</i>) proteins, and a molecular tool can easily provide this differentiation. Currently, molecular analysis can identify these species through genetic sequencing, an expensive and time-consuming process. In our study, after sequencing part of the mtDNA cytochrome c oxidase I (<i>COI</i>) gene and based on the differences found in the gene of each species, a set of species-specific primers was developed: one reverse primer common to both species and two forward primers, specific to each species, amplifying fragments of 199 base pairs (bp) for <i>C. includens</i> and 299 bp for <i>R. nu</i>. Our results indicate that the primers were specific for these species, enabling the identification of individuals directly through agarose gel. The new methodology proved to be accurate, rapid, and reliable for the correct identification of these two species of loopers.https://www.mdpi.com/2075-4450/15/12/969Plusiinaesoybean loopersunflower loopermtCOIPCRmolecular identification |
| spellingShingle | Guilherme A. Gotardi Natália R. F. Batista Tamylin Kaori Ishizuka Luiz H. Marques Mário H. Dal Pogetto Amit Sethi Mark L. Dahmer Timothy Nowatzki A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> Insects Plusiinae soybean looper sunflower looper mtCOI PCR molecular identification |
| title | A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> |
| title_full | A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> |
| title_fullStr | A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> |
| title_full_unstemmed | A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> |
| title_short | A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i> |
| title_sort | novel polymerase chain reaction pcr based method for the rapid identification of i chrysodeixis includens i and i rachiplusia nu i |
| topic | Plusiinae soybean looper sunflower looper mtCOI PCR molecular identification |
| url | https://www.mdpi.com/2075-4450/15/12/969 |
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