Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparedness
Introduction: A key factor in influenza pandemic preparedness is the ability to detect zoonotic influenza virus strains as they emerge in humans through spillover events, ideally before human-to-human transmission occurs. Design: In this study, the utility of the QIAstat-Dx syndromic device for infl...
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Elsevier
2024-12-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024166765 |
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| author | Luis Peñarrubia Sven Reister Sara Jiménez-Guzmán Roberto Porco Clàudia Congost-Teixidor Gemma Pueyo Carla Camprubí-Font Katariina Vara Maria de la Cruz Cardenosa Maria Contreras Aida Mayorgas Frederick van Deursen Dietrich Lueerssen Marti Juanola-Falgarona Martin Schwemmle Kevin Ciminski Davide Manissero |
| author_facet | Luis Peñarrubia Sven Reister Sara Jiménez-Guzmán Roberto Porco Clàudia Congost-Teixidor Gemma Pueyo Carla Camprubí-Font Katariina Vara Maria de la Cruz Cardenosa Maria Contreras Aida Mayorgas Frederick van Deursen Dietrich Lueerssen Marti Juanola-Falgarona Martin Schwemmle Kevin Ciminski Davide Manissero |
| author_sort | Luis Peñarrubia |
| collection | DOAJ |
| description | Introduction: A key factor in influenza pandemic preparedness is the ability to detect zoonotic influenza virus strains as they emerge in humans through spillover events, ideally before human-to-human transmission occurs. Design: In this study, the utility of the QIAstat-Dx syndromic device for influenza surveillance was evaluated. Bioinformatic analysis was performed on all WHO-recommended influenza Candidate Vaccine Viruses (CVVs), including the common strains recommended for the 2023–2024 influenza vaccine composition in the northern hemisphere, and 16 different H5 highly pathogenic avian influenza virus (HPAIV) and two H9N2 low pathogenic avian influenza virus (LPAIV) strains. For laboratory validation, engineered gene fragments and real HPAIV and LPAIV samples were tested using the QIAstat-Dx Respiratory SARS-CoV-2 Panel. Results: During the bioinformatic screening, common influenza strains were positive including influenza A subtypes, and all H5 HPAIV and LPAIV H9N2 were detected as Influenza A positive without subtype discrimination. In all cases, laboratory validation confirmed all bioinformatic results. Conclusion: QIAstat-Dx can detect all tested potentially zoonotic influenza A virus strains, and discriminate them from human sesonal influenza A viruses, ensuring a correct diagnosis. Any tool available for surveillance and pandemic preparedness is essential for a rapid response to reduce healthcare costs and severity of future influenza pandemics. |
| format | Article |
| id | doaj-art-3abed2689ad44e7dab4e96fe4e1db491 |
| institution | OA Journals |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-3abed2689ad44e7dab4e96fe4e1db4912025-08-20T01:59:34ZengElsevierHeliyon2405-84402024-12-011023e4064510.1016/j.heliyon.2024.e40645Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparednessLuis Peñarrubia0Sven Reister1Sara Jiménez-Guzmán2Roberto Porco3Clàudia Congost-Teixidor4Gemma Pueyo5Carla Camprubí-Font6Katariina Vara7Maria de la Cruz Cardenosa8Maria Contreras9Aida Mayorgas10Frederick van Deursen11Dietrich Lueerssen12Marti Juanola-Falgarona13Martin Schwemmle14Kevin Ciminski15Davide Manissero16STAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, Spain; Corresponding author.QIAGEN GmbH, Qiagenstr.1 40724 Hilden, GermanySTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainQIAGEN Manchester Ltd, Citylabs 2.0, Hathersage Road, Manchester, M13 0BH, UKSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainSTAT-Dx Life S.L. (A QIAGEN Company) Baldiri Reixac, 4 Barcelona 08028, SpainInstitute of Virology, Medical Center University of Freiburg, Freiburg, Germany; Faculty of Medicine, University of Freiburg, Freiburg, GermanyInstitute of Virology, Medical Center University of Freiburg, Freiburg, Germany; Faculty of Medicine, University of Freiburg, Freiburg, GermanyQIAGEN Manchester Ltd, Citylabs 2.0, Hathersage Road, Manchester, M13 0BH, UKIntroduction: A key factor in influenza pandemic preparedness is the ability to detect zoonotic influenza virus strains as they emerge in humans through spillover events, ideally before human-to-human transmission occurs. Design: In this study, the utility of the QIAstat-Dx syndromic device for influenza surveillance was evaluated. Bioinformatic analysis was performed on all WHO-recommended influenza Candidate Vaccine Viruses (CVVs), including the common strains recommended for the 2023–2024 influenza vaccine composition in the northern hemisphere, and 16 different H5 highly pathogenic avian influenza virus (HPAIV) and two H9N2 low pathogenic avian influenza virus (LPAIV) strains. For laboratory validation, engineered gene fragments and real HPAIV and LPAIV samples were tested using the QIAstat-Dx Respiratory SARS-CoV-2 Panel. Results: During the bioinformatic screening, common influenza strains were positive including influenza A subtypes, and all H5 HPAIV and LPAIV H9N2 were detected as Influenza A positive without subtype discrimination. In all cases, laboratory validation confirmed all bioinformatic results. Conclusion: QIAstat-Dx can detect all tested potentially zoonotic influenza A virus strains, and discriminate them from human sesonal influenza A viruses, ensuring a correct diagnosis. Any tool available for surveillance and pandemic preparedness is essential for a rapid response to reduce healthcare costs and severity of future influenza pandemics.http://www.sciencedirect.com/science/article/pii/S2405844024166765Influenza pandemic preparednessHigh pathogenic avian influenzaQIAstat-dx respiratory SARS-CoV-2 panelInfluenza a H5 detectionInfluenza surveillance |
| spellingShingle | Luis Peñarrubia Sven Reister Sara Jiménez-Guzmán Roberto Porco Clàudia Congost-Teixidor Gemma Pueyo Carla Camprubí-Font Katariina Vara Maria de la Cruz Cardenosa Maria Contreras Aida Mayorgas Frederick van Deursen Dietrich Lueerssen Marti Juanola-Falgarona Martin Schwemmle Kevin Ciminski Davide Manissero Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparedness Heliyon Influenza pandemic preparedness High pathogenic avian influenza QIAstat-dx respiratory SARS-CoV-2 panel Influenza a H5 detection Influenza surveillance |
| title | Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparedness |
| title_full | Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparedness |
| title_fullStr | Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparedness |
| title_full_unstemmed | Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparedness |
| title_short | Molecular diagnostics using the QIAstat-Dx syndromic device for covering avian influenza pandemic preparedness |
| title_sort | molecular diagnostics using the qiastat dx syndromic device for covering avian influenza pandemic preparedness |
| topic | Influenza pandemic preparedness High pathogenic avian influenza QIAstat-dx respiratory SARS-CoV-2 panel Influenza a H5 detection Influenza surveillance |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024166765 |
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