Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin
[Objective] To identify the ABO blood group of a patient with rare B antigen-specific autoantibody with cold agglutinin, and evaluate the effect of blood transfusion. [Methods] Red blood cells of patient were washed with 37℃ physiological saline and treated with sulfhydryl reagent. ABO blood group a...
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-01-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.021&lang=en |
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| _version_ | 1832585121871429632 |
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| author | YANG Linyu CHEN LI LI Zhongjun |
| author_facet | YANG Linyu CHEN LI LI Zhongjun |
| author_sort | YANG Linyu |
| collection | DOAJ |
| description | [Objective] To identify the ABO blood group of a patient with rare B antigen-specific autoantibody with cold agglutinin, and evaluate the effect of blood transfusion. [Methods] Red blood cells of patient were washed with 37℃ physiological saline and treated with sulfhydryl reagent. ABO blood group antigen was detected by tube method and microcolumn gel method. After the cold agglutinin was removed by EDTA anticoagulant plasma absorbed by type O red blood cells at 4℃, the related blood group antibodies were detected by type B red blood cells absorbing and releasing liquid at 4℃. The blood transfusion effect of patients was evaluated by the changes of hemoglobin before and after transfusion, and their ABO blood group was continuously monitored. [Results] B antigen was detected in the positive setting of serological experiment, cold agglutinin was detected by absorption and elution of type O red blood cells, and anti-B antibody was detected by absorption and elution of type B red blood cells. That is, there was specific autoantibody against B antigen, and the antibody property was IgM. No adverse reactions occurred during the infusion of 3 U type O washed red blood cells and the infusion was effective. The patient was continuously followed for two months, and the forward and reverse blood group identification were consistent, both of which were type B. [Conclusion] According to the previous blood group identification results, serological identification and follow-up comprehensive analysis, the ABO blood group of the patient is type B, but there are transient high titer cold agglutinin and B antigen-specific autoantibodies. |
| format | Article |
| id | doaj-art-3aa84b4d883d444d94d64359d00f7904 |
| institution | Kabale University |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-3aa84b4d883d444d94d64359d00f79042025-01-27T01:46:12ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-01-0138112212510.13303/j.cjbt.issn.1004-549x.2025.01.0211004-549X(2025)1-0122-04Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutininYANG Linyu0CHEN LI1LI Zhongjun2The Second Affiliated Hospital of the Army Medical University, Chongqing 400037, ChinaThe Second Affiliated Hospital of the Army Medical University, Chongqing 400037, ChinaThe Second Affiliated Hospital of the Army Medical University, Chongqing 400037, China[Objective] To identify the ABO blood group of a patient with rare B antigen-specific autoantibody with cold agglutinin, and evaluate the effect of blood transfusion. [Methods] Red blood cells of patient were washed with 37℃ physiological saline and treated with sulfhydryl reagent. ABO blood group antigen was detected by tube method and microcolumn gel method. After the cold agglutinin was removed by EDTA anticoagulant plasma absorbed by type O red blood cells at 4℃, the related blood group antibodies were detected by type B red blood cells absorbing and releasing liquid at 4℃. The blood transfusion effect of patients was evaluated by the changes of hemoglobin before and after transfusion, and their ABO blood group was continuously monitored. [Results] B antigen was detected in the positive setting of serological experiment, cold agglutinin was detected by absorption and elution of type O red blood cells, and anti-B antibody was detected by absorption and elution of type B red blood cells. That is, there was specific autoantibody against B antigen, and the antibody property was IgM. No adverse reactions occurred during the infusion of 3 U type O washed red blood cells and the infusion was effective. The patient was continuously followed for two months, and the forward and reverse blood group identification were consistent, both of which were type B. [Conclusion] According to the previous blood group identification results, serological identification and follow-up comprehensive analysis, the ABO blood group of the patient is type B, but there are transient high titer cold agglutinin and B antigen-specific autoantibodies.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.021&lang=enabo blood groupautoantibodiescold agglutininantibody identification |
| spellingShingle | YANG Linyu CHEN LI LI Zhongjun Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin Zhongguo shuxue zazhi abo blood group autoantibodies cold agglutinin antibody identification |
| title | Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin |
| title_full | Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin |
| title_fullStr | Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin |
| title_full_unstemmed | Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin |
| title_short | Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin |
| title_sort | pre transfusion detection and transfusion therapy of a patient with autoanti b and cold agglutinin |
| topic | abo blood group autoantibodies cold agglutinin antibody identification |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.021&lang=en |
| work_keys_str_mv | AT yanglinyu pretransfusiondetectionandtransfusiontherapyofapatientwithautoantibandcoldagglutinin AT chenli pretransfusiondetectionandtransfusiontherapyofapatientwithautoantibandcoldagglutinin AT lizhongjun pretransfusiondetectionandtransfusiontherapyofapatientwithautoantibandcoldagglutinin |