Rapid, user-friendly, cost-effective DNA and library Preparation methods for whole-genome sequencing of bacteria with varying cell wall composition and GC content using minimal DNA on the illumina platform

Rapid, user-friendly, cost-effective DNA and library Preparation methods for whole-genome sequencing of bacteria with varying cell wall composition and GC content using minimal DNA on the illumina platform

Abstract Background Whole-genome sequencing using high-throughput sequencing is essential for identifying and characterising chromosomes and plasmids in nosocomial and environmental bacterial pathogens, including those with bioterrorism potential. To expedite outbreaks investigations, including acci...

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Bibliographic Details
Main Authors: Bertrand Bearzatto, Jean-François Durant, Jérôme Ambroise, Jean-Luc Gala
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Genomics
Subjects:
ATCC bacteria
Bacillus
Library preparation kits
Extraction methods
Whole genome sequencing
Plasmid
Online Access:https://doi.org/10.1186/s12864-025-11598-7
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