Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe
Acanthamoeba species are the major cause of Acanthamoeba Keratitis (AK) of eyes, Acanthamoeba meningoencephalitis of the central nervous system, and fatal Granulomatous Amoebic Encephalitis (GAE) of the brain in humans. These diseases are difficult to treat due to their resistance to extreme temper...
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PAGEPress Publications
2024-09-01
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| Series: | Infectious Diseases and Herbal Medicine |
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| author | Kudzai Fambisai Petros Muchesa Farisai Chidzwondo Rumbidzai Mangoyi |
| author_facet | Kudzai Fambisai Petros Muchesa Farisai Chidzwondo Rumbidzai Mangoyi |
| author_sort | Kudzai Fambisai |
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Acanthamoeba species are the major cause of Acanthamoeba Keratitis (AK) of eyes, Acanthamoeba meningoencephalitis of the central nervous system, and fatal Granulomatous Amoebic Encephalitis (GAE) of the brain in humans. These diseases are difficult to treat due to their resistance to extreme temperatures, pH, alcohol, and pressure. This research aimed to determine the anti-amoebic effects of some selected plant extracts against Acanthamoeba species isolated from borehole water samples from Budiriro District, Harare, Zimbabwe. Acanthamoeba castellannii species were isolated and confirmed present in Budiriro borehole water samples using non-nutrient agar, microscope, real-time Polymerase Chain Reaction (PCR), and gel electrophoresis. The selected plant samples, Murumanyama (Xeroderris stuhlmannii) bark extract, Munhundurwa (Solanum incanum) fruit extract and Mufandichimuka (Myrothamnus flabellifolius) stem and leaf extracts were then investigated for their anti-amoebic effects against the isolated Acanthamoeba castellannii, using agar well diffusion method. Chlorhexidine gluconate antibiotic was used as a control. The results show that Acanthamoeba castellannii is the most common Acanthamoeba species in borehole water in Budiriro District in Harare and all the tested plant samples had no anti-amoebic effects against this isolated Acanthamoeba castellannii.
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| format | Article |
| id | doaj-art-3a827c058a82490e92b59ee095ae81b2 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-09-01 |
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| series | Infectious Diseases and Herbal Medicine |
| spelling | doaj-art-3a827c058a82490e92b59ee095ae81b22025-02-06T13:43:01ZengPAGEPress PublicationsInfectious Diseases and Herbal Medicine2724-52842024-09-0151Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, ZimbabweKudzai Fambisai0Petros Muchesa1Farisai Chidzwondo2Rumbidzai Mangoyi3https://orcid.org/0000-0002-0663-3058Department of Biotechnology and Biochemistry, University of Zimbabwe, HarareDepartment of Biotechnology and Biochemistry, University of Zimbabwe, HarareDepartment of Biotechnology and Biochemistry, University of Zimbabwe, HarareDepartment of Biotechnology and Biochemistry, University of Zimbabwe, Harare Acanthamoeba species are the major cause of Acanthamoeba Keratitis (AK) of eyes, Acanthamoeba meningoencephalitis of the central nervous system, and fatal Granulomatous Amoebic Encephalitis (GAE) of the brain in humans. These diseases are difficult to treat due to their resistance to extreme temperatures, pH, alcohol, and pressure. This research aimed to determine the anti-amoebic effects of some selected plant extracts against Acanthamoeba species isolated from borehole water samples from Budiriro District, Harare, Zimbabwe. Acanthamoeba castellannii species were isolated and confirmed present in Budiriro borehole water samples using non-nutrient agar, microscope, real-time Polymerase Chain Reaction (PCR), and gel electrophoresis. The selected plant samples, Murumanyama (Xeroderris stuhlmannii) bark extract, Munhundurwa (Solanum incanum) fruit extract and Mufandichimuka (Myrothamnus flabellifolius) stem and leaf extracts were then investigated for their anti-amoebic effects against the isolated Acanthamoeba castellannii, using agar well diffusion method. Chlorhexidine gluconate antibiotic was used as a control. The results show that Acanthamoeba castellannii is the most common Acanthamoeba species in borehole water in Budiriro District in Harare and all the tested plant samples had no anti-amoebic effects against this isolated Acanthamoeba castellannii. https://www.pagepress.org/medicine/idhm/article/view/391Acanthamoeba castellanniiSolanum incanumXeroderris stuhlmanniiMyrothamnus flabellifoliusanti-amoebic effectsplant extracts |
| spellingShingle | Kudzai Fambisai Petros Muchesa Farisai Chidzwondo Rumbidzai Mangoyi Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe Infectious Diseases and Herbal Medicine Acanthamoeba castellannii Solanum incanum Xeroderris stuhlmannii Myrothamnus flabellifolius anti-amoebic effects plant extracts |
| title | Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe |
| title_full | Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe |
| title_fullStr | Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe |
| title_full_unstemmed | Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe |
| title_short | Anti-amoebic effects of selected herbal extracts against Acanthamoeba species isolated from different borehole water samples from Budiriro District in Harare, Zimbabwe |
| title_sort | anti amoebic effects of selected herbal extracts against acanthamoeba species isolated from different borehole water samples from budiriro district in harare zimbabwe |
| topic | Acanthamoeba castellannii Solanum incanum Xeroderris stuhlmannii Myrothamnus flabellifolius anti-amoebic effects plant extracts |
| url | https://www.pagepress.org/medicine/idhm/article/view/391 |
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