In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz
Ostericum koreanum maximowicz (Umbelliferae), a medicinal herb in Korean Oriental Medicine, has been applied to treat cold, headache, neuralgia and arthralgia. The ethyl acetate fraction of O. koreanum root was subjected to in vitro antioxidant activity with different methods for free radical scaven...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/183172 |
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| author | Ramalingam Mahesh Hyo Won Jung Jun Hong Park Yong-Ki Park |
| author_facet | Ramalingam Mahesh Hyo Won Jung Jun Hong Park Yong-Ki Park |
| author_sort | Ramalingam Mahesh |
| collection | DOAJ |
| description | Ostericum koreanum maximowicz (Umbelliferae), a medicinal herb in Korean Oriental Medicine, has been applied to treat cold, headache, neuralgia and arthralgia. The ethyl acetate fraction of O. koreanum root was subjected to in vitro antioxidant activity with different methods for free radical scavenging activities. In addition, the cell viability and nitric oxide release assays were performed here for the first time in neuroblastoma (Neuro-2a) cell cultures. Among all the tested methods, the ethyl acetate fraction was expressed very active, exhibiting a good Trolox equivalent values and IC50, comparable to that of the commercial antioxidants, Trolox and ascorbic acid, respectively. The results showed that there was a reduction of cell viability by the fraction in a concentration dependent manner. These results suggest that O. koreanum shows good antioxidant activities in vitro by inhibiting free radicals. These findings provide a rationale for the in vivo testing. Also, the major constituents behind the antioxidant mechanisms of this fraction warrant further study. |
| format | Article |
| id | doaj-art-3a73a623b02546fea0f9b9306d26ce0b |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-3a73a623b02546fea0f9b9306d26ce0b2025-02-03T06:01:05ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01831451145510.1155/2011/183172In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum MaximowiczRamalingam Mahesh0Hyo Won Jung1Jun Hong Park2Yong-Ki Park3Oriental Medicine Research Institute, Dongguk University, 707, Seokjangdong Gyeongju, Gyeongbuk 780-714, Republic of KoreaOriental Medicine R&D Center and Department of Herbology, College of Oriental Medicine, Dongguk University, 707, Seokjangdong Gyeongju, Gyeongbuk 780-714, Republic of KoreaBonghwa Alpine Medicinal Plant Experiment Station, Gyeongsangbook-do 755-843, Republic of KoreaOriental Medicine Research Institute, Dongguk University, 707, Seokjangdong Gyeongju, Gyeongbuk 780-714, Republic of KoreaOstericum koreanum maximowicz (Umbelliferae), a medicinal herb in Korean Oriental Medicine, has been applied to treat cold, headache, neuralgia and arthralgia. The ethyl acetate fraction of O. koreanum root was subjected to in vitro antioxidant activity with different methods for free radical scavenging activities. In addition, the cell viability and nitric oxide release assays were performed here for the first time in neuroblastoma (Neuro-2a) cell cultures. Among all the tested methods, the ethyl acetate fraction was expressed very active, exhibiting a good Trolox equivalent values and IC50, comparable to that of the commercial antioxidants, Trolox and ascorbic acid, respectively. The results showed that there was a reduction of cell viability by the fraction in a concentration dependent manner. These results suggest that O. koreanum shows good antioxidant activities in vitro by inhibiting free radicals. These findings provide a rationale for the in vivo testing. Also, the major constituents behind the antioxidant mechanisms of this fraction warrant further study.http://dx.doi.org/10.1155/2011/183172 |
| spellingShingle | Ramalingam Mahesh Hyo Won Jung Jun Hong Park Yong-Ki Park In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz E-Journal of Chemistry |
| title | In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz |
| title_full | In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz |
| title_fullStr | In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz |
| title_full_unstemmed | In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz |
| title_short | In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz |
| title_sort | in vitro antioxidant capacity and neuronal cell toxicity of roots of ostericum koreanum maximowicz |
| url | http://dx.doi.org/10.1155/2011/183172 |
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