Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry
Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of ch...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/695658 |
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| author | Anna W. Sobańska Katarzyna Kałębasiak Jarosław Pyzowski Elżbieta Brzezińska |
| author_facet | Anna W. Sobańska Katarzyna Kałębasiak Jarosław Pyzowski Elżbieta Brzezińska |
| author_sort | Anna W. Sobańska |
| collection | DOAJ |
| description | Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R>0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot−1, respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R>0.9998. The chromatographic method was fully validated. |
| format | Article |
| id | doaj-art-3a64e710ee9e4a44a4893d0b8a0e6314 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-3a64e710ee9e4a44a4893d0b8a0e63142025-02-03T01:33:08ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/695658695658Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV SpectrophotometryAnna W. Sobańska0Katarzyna Kałębasiak1Jarosław Pyzowski2Elżbieta Brzezińska3Department of Analytical Chemistry, Medical University of Lodz, Ulica Muszynskiego 1, 90-151 Lodz, PolandDepartment of Analytical Chemistry, Medical University of Lodz, Ulica Muszynskiego 1, 90-151 Lodz, PolandDepartment of Analytical Chemistry, Medical University of Lodz, Ulica Muszynskiego 1, 90-151 Lodz, PolandDepartment of Analytical Chemistry, Medical University of Lodz, Ulica Muszynskiego 1, 90-151 Lodz, PolandBenzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R>0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot−1, respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R>0.9998. The chromatographic method was fully validated.http://dx.doi.org/10.1155/2015/695658 |
| spellingShingle | Anna W. Sobańska Katarzyna Kałębasiak Jarosław Pyzowski Elżbieta Brzezińska Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry Journal of Analytical Methods in Chemistry |
| title | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
| title_full | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
| title_fullStr | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
| title_full_unstemmed | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
| title_short | Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry |
| title_sort | quantification of sunscreen benzophenone 4 in hair shampoos by hydrophilic interactions thin layer chromatography densitometry or derivative uv spectrophotometry |
| url | http://dx.doi.org/10.1155/2015/695658 |
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