The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping
Ideal agents for the topical treatment of skin wounds should have antimicrobial efficacy without negative influence on wound healing. Octenidine (OCT) has become a widely used antiseptic in professional wound care, but its influence on several components of the wound healing process remains unclear....
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/5143635 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559199751503872 |
|---|---|
| author | Nenad Nikolić Philip Kienzl Pooja Tajpara Martin Vierhapper Johannes Matiasek Adelheid Elbe-Bürger |
| author_facet | Nenad Nikolić Philip Kienzl Pooja Tajpara Martin Vierhapper Johannes Matiasek Adelheid Elbe-Bürger |
| author_sort | Nenad Nikolić |
| collection | DOAJ |
| description | Ideal agents for the topical treatment of skin wounds should have antimicrobial efficacy without negative influence on wound healing. Octenidine (OCT) has become a widely used antiseptic in professional wound care, but its influence on several components of the wound healing process remains unclear. In the present study, we have used a superficial wound model using tape stripping on human full-thickness skin ex vivo to investigate the influence of OCT on epidermal Langerhans cells (LCs) and cytokine secretion pattern of skin cells during wound healing in a model without disruption of the normal skin structure. Histological and immunofluorescence studies showed that OCT neither altered human skin architecture nor the viability of skin cells upon 48 hours of culture in unwounded or wounded skin. The epidermis of explants and LCs remained morphologically intact throughout the whole culture period upon OCT treatment. OCT inhibited the upregulation of the maturation marker CD83 on LCs and prevented their emigration in wounded skin. Furthermore, OCT reduced both pro- and anti-inflammatory mediators (IL-8, IL-33, and IL-10), while angiogenesis and growth factor mediators (VEGF and TGF-β1) remained unchanged in skin explant cultures. Our data provide novel insights into the host response to OCT in the biologically relevant environment of viable human (wounded) skin. |
| format | Article |
| id | doaj-art-3a59dd6e22e5450fb0667898bc091815 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-3a59dd6e22e5450fb0667898bc0918152025-02-03T01:30:35ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/51436355143635The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape StrippingNenad Nikolić0Philip Kienzl1Pooja Tajpara2Martin Vierhapper3Johannes Matiasek4Adelheid Elbe-Bürger5Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, AustriaDepartment of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, AustriaDepartment of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, AustriaDepartment of Surgery, Division of Plastic and Reconstructive Surgery, Medical University of Vienna, AustriaDepartment of Plastic, Aesthetic and Reconstructive Surgery, St. Josef Hospital, Vienna, AustriaDepartment of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, AustriaIdeal agents for the topical treatment of skin wounds should have antimicrobial efficacy without negative influence on wound healing. Octenidine (OCT) has become a widely used antiseptic in professional wound care, but its influence on several components of the wound healing process remains unclear. In the present study, we have used a superficial wound model using tape stripping on human full-thickness skin ex vivo to investigate the influence of OCT on epidermal Langerhans cells (LCs) and cytokine secretion pattern of skin cells during wound healing in a model without disruption of the normal skin structure. Histological and immunofluorescence studies showed that OCT neither altered human skin architecture nor the viability of skin cells upon 48 hours of culture in unwounded or wounded skin. The epidermis of explants and LCs remained morphologically intact throughout the whole culture period upon OCT treatment. OCT inhibited the upregulation of the maturation marker CD83 on LCs and prevented their emigration in wounded skin. Furthermore, OCT reduced both pro- and anti-inflammatory mediators (IL-8, IL-33, and IL-10), while angiogenesis and growth factor mediators (VEGF and TGF-β1) remained unchanged in skin explant cultures. Our data provide novel insights into the host response to OCT in the biologically relevant environment of viable human (wounded) skin.http://dx.doi.org/10.1155/2019/5143635 |
| spellingShingle | Nenad Nikolić Philip Kienzl Pooja Tajpara Martin Vierhapper Johannes Matiasek Adelheid Elbe-Bürger The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping Journal of Immunology Research |
| title | The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping |
| title_full | The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping |
| title_fullStr | The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping |
| title_full_unstemmed | The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping |
| title_short | The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping |
| title_sort | antiseptic octenidine inhibits langerhans cell activation and modulates cytokine expression upon superficial wounding with tape stripping |
| url | http://dx.doi.org/10.1155/2019/5143635 |
| work_keys_str_mv | AT nenadnikolic theantisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT philipkienzl theantisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT poojatajpara theantisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT martinvierhapper theantisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT johannesmatiasek theantisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT adelheidelbeburger theantisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT nenadnikolic antisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT philipkienzl antisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT poojatajpara antisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT martinvierhapper antisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT johannesmatiasek antisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping AT adelheidelbeburger antisepticoctenidineinhibitslangerhanscellactivationandmodulatescytokineexpressionuponsuperficialwoundingwithtapestripping |