Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli
Abstract Bacterial infections stand as prominent contributors to global mortality and morbidity rates. Harnessing the potential antimicrobial activity of secondary metabolites derived from natural sources holds promise for developing novel therapeutic drugs. Streptomyces spp. represents pivotal micr...
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Academia Brasileira de Ciências
2025-04-01
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| author | KELI C.C. BARROSO VERIDIANA G. VIRGINIO BENI J.M. CHAÚQUE VINICIUS J. MASCHIO SUELI T. VAN DER SAND MARILISE B. ROTT |
| author_facet | KELI C.C. BARROSO VERIDIANA G. VIRGINIO BENI J.M. CHAÚQUE VINICIUS J. MASCHIO SUELI T. VAN DER SAND MARILISE B. ROTT |
| author_sort | KELI C.C. BARROSO |
| collection | DOAJ |
| description | Abstract Bacterial infections stand as prominent contributors to global mortality and morbidity rates. Harnessing the potential antimicrobial activity of secondary metabolites derived from natural sources holds promise for developing novel therapeutic drugs. Streptomyces spp. represents pivotal microorganisms in the synthesis of these compounds. Acanthamoeba spp. serves as natural virulence amplifiers for a wide range of bacterial pathogens. This study evaluates the antimicrobial efficacy of crude extracts of Streptomyces sp. cocultured trials with Acanthamoeba polyphaga against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli. The production of crude extracts from Streptomyces sp. was monitored over 28 days. The antimicrobial activity against P. aeruginosa and E. coli was evaluated by measuring the inhibitory halos. Viability amoebae and bacteria were assessed. A slight decrease in the viability of A. polyphaga was noted during the coculture. Conversely, coculture promoted bacterial growth and facilitated the synthesis of extracts that showed antimicrobial effects against P. aeruginosa and E. coli, while showing no impact on amoebae. The extracts were active mainly against P. aeruginosa. The findings show that the interaction between A. polyphaga and Streptomyces sp. modulates the production of antimicrobial secondary metabolites by bacteria. Further investigations are needed to characterize the nature of this modulation, and the bactericidal components. |
| format | Article |
| id | doaj-art-3a44c7c6b74d43a698ab7ce89f76f7b8 |
| institution | DOAJ |
| issn | 1678-2690 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj-art-3a44c7c6b74d43a698ab7ce89f76f7b82025-08-20T03:14:53ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902025-04-0197210.1590/0001-3765202520240655Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coliKELI C.C. BARROSOhttps://orcid.org/0000-0003-4386-9921VERIDIANA G. VIRGINIOhttps://orcid.org/0000-0003-1530-7841BENI J.M. CHAÚQUEhttps://orcid.org/0000-0002-9440-3227VINICIUS J. MASCHIOhttps://orcid.org/0000-0002-2655-5886SUELI T. VAN DER SANDhttps://orcid.org/0000-0002-8591-6514MARILISE B. ROTThttps://orcid.org/0000-0002-5227-4594Abstract Bacterial infections stand as prominent contributors to global mortality and morbidity rates. Harnessing the potential antimicrobial activity of secondary metabolites derived from natural sources holds promise for developing novel therapeutic drugs. Streptomyces spp. represents pivotal microorganisms in the synthesis of these compounds. Acanthamoeba spp. serves as natural virulence amplifiers for a wide range of bacterial pathogens. This study evaluates the antimicrobial efficacy of crude extracts of Streptomyces sp. cocultured trials with Acanthamoeba polyphaga against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli. The production of crude extracts from Streptomyces sp. was monitored over 28 days. The antimicrobial activity against P. aeruginosa and E. coli was evaluated by measuring the inhibitory halos. Viability amoebae and bacteria were assessed. A slight decrease in the viability of A. polyphaga was noted during the coculture. Conversely, coculture promoted bacterial growth and facilitated the synthesis of extracts that showed antimicrobial effects against P. aeruginosa and E. coli, while showing no impact on amoebae. The extracts were active mainly against P. aeruginosa. The findings show that the interaction between A. polyphaga and Streptomyces sp. modulates the production of antimicrobial secondary metabolites by bacteria. Further investigations are needed to characterize the nature of this modulation, and the bactericidal components.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000200902&lng=en&tlng=enAcanthamoeba polyphagaStreptomyces spcoculturecrude extractantimicrobial secondary metabolites |
| spellingShingle | KELI C.C. BARROSO VERIDIANA G. VIRGINIO BENI J.M. CHAÚQUE VINICIUS J. MASCHIO SUELI T. VAN DER SAND MARILISE B. ROTT Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli Anais da Academia Brasileira de Ciências Acanthamoeba polyphaga Streptomyces sp coculture crude extract antimicrobial secondary metabolites |
| title | Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli |
| title_full | Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli |
| title_fullStr | Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli |
| title_full_unstemmed | Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli |
| title_short | Coculturing Streptomyces sp. with Acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug-resistant Pseudomonas aeruginosa and Escherichia coli |
| title_sort | coculturing streptomyces sp with acanthamoeba polyphaga enhances the antimicrobial effectiveness of its crude extract against multidrug resistant pseudomonas aeruginosa and escherichia coli |
| topic | Acanthamoeba polyphaga Streptomyces sp coculture crude extract antimicrobial secondary metabolites |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000200902&lng=en&tlng=en |
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