DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONS
A successful attempt was made through this investigation to develop a cost-effective protocol for two varieties of carnation (Dianthus caryophyllus L.) ornamental plants named Clove Pink and Scarlet. Healthy and vigorous cultures were established by inoculating axillary buds on MS medium after a su...
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University of Zakho
2025-01-01
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| Series: | Science Journal of University of Zakho |
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| Online Access: | http://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/1423 |
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| author | Lolav Rajab Al-Mizory Rafail S. Toma Fareed Khalid Yaseen |
| author_facet | Lolav Rajab Al-Mizory Rafail S. Toma Fareed Khalid Yaseen |
| author_sort | Lolav Rajab Al-Mizory |
| collection | DOAJ |
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A successful attempt was made through this investigation to develop a cost-effective protocol for two varieties of carnation (Dianthus caryophyllus L.) ornamental plants named Clove Pink and Scarlet. Healthy and vigorous cultures were established by inoculating axillary buds on MS medium after a successful disinfestation process by sterilizing in 2.5% NaOCl for 10 minutes. The microshoots were multiplied by testing different benzyladenine (BA) and kinetin concentrations. Luckily, the best multiplication parameters were achieved with MS medium free of growth regulators, which will reduce the propagation costs. The higher number of shoots (11.60 shoots/ explant), leaves (59.80 leaves/ explant), and longest shoots (10.90 cm) when grown on MS medium without Plant growth regulators (PGRs), Additionally, the clove pink variety on MS medium with 1.5 mg l-1 BA and Scarlet variety grown on MS-free of PGRs respectively. A 100% percent rooting was achieved for both varieties when 1.5 mg l-1 Naphthalene acetic acid (NAA) was added to the culture media. The same NAA concentration for scarlet variety microshoots resulted in the highest number of roots (26.2 roots/explant) and the longest roots (9.0 cm). Successful acclimatization was observed by transferring the well-rooted plantlets to greenhouse conditions while putting them in peat moss
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| format | Article |
| id | doaj-art-3a2ad3da35394267b56fff8a2b9fb8a8 |
| institution | Kabale University |
| issn | 2663-628X 2663-6298 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | University of Zakho |
| record_format | Article |
| series | Science Journal of University of Zakho |
| spelling | doaj-art-3a2ad3da35394267b56fff8a2b9fb8a82025-01-31T02:39:09ZengUniversity of ZakhoScience Journal of University of Zakho2663-628X2663-62982025-01-0113110.25271/sjuoz.2025.13.1.1423DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONSLolav Rajab Al-Mizory0Rafail S. Toma1Fareed Khalid Yaseen2Department of Horticulture, College of Agricultural Engineering Sciences, University of DuhokDepartment of Horticulture, College of Agricultural Engineering Sciences, University of DuhokDepartment of General Science, College of Basic Education, University of Zakho, Zakho, 42002, Dohuk, Kurdistan Region-Iraq A successful attempt was made through this investigation to develop a cost-effective protocol for two varieties of carnation (Dianthus caryophyllus L.) ornamental plants named Clove Pink and Scarlet. Healthy and vigorous cultures were established by inoculating axillary buds on MS medium after a successful disinfestation process by sterilizing in 2.5% NaOCl for 10 minutes. The microshoots were multiplied by testing different benzyladenine (BA) and kinetin concentrations. Luckily, the best multiplication parameters were achieved with MS medium free of growth regulators, which will reduce the propagation costs. The higher number of shoots (11.60 shoots/ explant), leaves (59.80 leaves/ explant), and longest shoots (10.90 cm) when grown on MS medium without Plant growth regulators (PGRs), Additionally, the clove pink variety on MS medium with 1.5 mg l-1 BA and Scarlet variety grown on MS-free of PGRs respectively. A 100% percent rooting was achieved for both varieties when 1.5 mg l-1 Naphthalene acetic acid (NAA) was added to the culture media. The same NAA concentration for scarlet variety microshoots resulted in the highest number of roots (26.2 roots/explant) and the longest roots (9.0 cm). Successful acclimatization was observed by transferring the well-rooted plantlets to greenhouse conditions while putting them in peat moss http://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/1423Tissue CultureCulture Media CarnationDianthus Caryophyllus LCost-Effective ProtocolMicropropagation |
| spellingShingle | Lolav Rajab Al-Mizory Rafail S. Toma Fareed Khalid Yaseen DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONS Science Journal of University of Zakho Tissue Culture Culture Media Carnation Dianthus Caryophyllus L Cost-Effective Protocol Micropropagation |
| title | DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONS |
| title_full | DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONS |
| title_fullStr | DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONS |
| title_full_unstemmed | DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONS |
| title_short | DEVELOPING A COST-EFFECTIVE MICROPREDATION PROTOCOL FOR TWO CARNATION (DIANTHUS CARYOPHYLLUS L.) VARIETIES UNDER IN VITRO CONDITIONS |
| title_sort | developing a cost effective micropredation protocol for two carnation dianthus caryophyllus l varieties under in vitro conditions |
| topic | Tissue Culture Culture Media Carnation Dianthus Caryophyllus L Cost-Effective Protocol Micropropagation |
| url | http://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/1423 |
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