Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion Molecules
The present study investigated whether Helicobacter pylori water extract induces the upregulation of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin on human umbilical vein endothelial cells, using an ELISA. The nature of the substances mediating this upregulation...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2004-01-01
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| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/2004/158638 |
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| _version_ | 1832565371878506496 |
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| author | Norimasa Yoshida Kyoichi Kassai Hironobu Murase Yukiko Tanaka Satoshi Kokura Hiroshi Ichikawa Yuji Naito Takeshi Okanoue Toshikazu Yoshikawa |
| author_facet | Norimasa Yoshida Kyoichi Kassai Hironobu Murase Yukiko Tanaka Satoshi Kokura Hiroshi Ichikawa Yuji Naito Takeshi Okanoue Toshikazu Yoshikawa |
| author_sort | Norimasa Yoshida |
| collection | DOAJ |
| description | The present study investigated whether Helicobacter pylori water extract induces the upregulation of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin on human umbilical vein endothelial cells, using an ELISA. The nature of the substances mediating this upregulation was also analyzed. H pylori water extract derived from type strain (NCTC 11637) significantly upregulated intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin to the same extent as interleukin-1. Treatments with extracts from clinical strains showed no significant increases in expression of these adhesion molecules. In a fractionation study, approximately 7 kDa fraction showed peak activity. This activity was tolerant to heating and trypsin digestion. These results indicate that H pylori water extract contains water-soluble, low-molecular, nonprotein substances which induce upregulation of adhesion molecules on human umbilical vein endothelial cells, suggesting that products of H pylori may elicit gastric mucosal inflammation by promoting expression of endothelial adhesion molecules. |
| format | Article |
| id | doaj-art-3a26126037284721b5e196788070a444 |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-3a26126037284721b5e196788070a4442025-02-03T01:07:54ZengWileyCanadian Journal of Gastroenterology0835-79002004-01-0118638739110.1155/2004/158638Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion MoleculesNorimasa Yoshida0Kyoichi Kassai1Hironobu Murase2Yukiko Tanaka3Satoshi Kokura4Hiroshi Ichikawa5Yuji Naito6Takeshi Okanoue7Toshikazu Yoshikawa8Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, JapanInflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, JapanCCI Corporation, Gifu, JapanInflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, JapanMolecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, JapanInflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, JapanMolecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, JapanMolecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, JapanInflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, JapanThe present study investigated whether Helicobacter pylori water extract induces the upregulation of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin on human umbilical vein endothelial cells, using an ELISA. The nature of the substances mediating this upregulation was also analyzed. H pylori water extract derived from type strain (NCTC 11637) significantly upregulated intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin to the same extent as interleukin-1. Treatments with extracts from clinical strains showed no significant increases in expression of these adhesion molecules. In a fractionation study, approximately 7 kDa fraction showed peak activity. This activity was tolerant to heating and trypsin digestion. These results indicate that H pylori water extract contains water-soluble, low-molecular, nonprotein substances which induce upregulation of adhesion molecules on human umbilical vein endothelial cells, suggesting that products of H pylori may elicit gastric mucosal inflammation by promoting expression of endothelial adhesion molecules.http://dx.doi.org/10.1155/2004/158638 |
| spellingShingle | Norimasa Yoshida Kyoichi Kassai Hironobu Murase Yukiko Tanaka Satoshi Kokura Hiroshi Ichikawa Yuji Naito Takeshi Okanoue Toshikazu Yoshikawa Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion Molecules Canadian Journal of Gastroenterology |
| title | Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion Molecules |
| title_full | Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion Molecules |
| title_fullStr | Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion Molecules |
| title_full_unstemmed | Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion Molecules |
| title_short | Effects of Helicobacter pylori Water Extract on Expression of Endothelial Adhesion Molecules |
| title_sort | effects of helicobacter pylori water extract on expression of endothelial adhesion molecules |
| url | http://dx.doi.org/10.1155/2004/158638 |
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