The Fluorescent Detection of Alkaline Phosphatase Based on Iron Nanoclusters and a Manganese Dioxide Nanosheet
Fluorescent iron nanoclusters are emerging fluorescent nanomaterials. Herein, we synthesized hemoglobin-coated iron nanoclusters (Hb−Fe NCs) with a significant fluorescence emission peak at 615 nm and investigated the inner-filter effect of fluorescence induced by a manganese dioxide nanosheet (MnO&...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/25/2/585 |
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| Summary: | Fluorescent iron nanoclusters are emerging fluorescent nanomaterials. Herein, we synthesized hemoglobin-coated iron nanoclusters (Hb−Fe NCs) with a significant fluorescence emission peak at 615 nm and investigated the inner-filter effect of fluorescence induced by a manganese dioxide nanosheet (MnO<sub>2</sub> NS). The fluorescence quenching of Hb−Fe NCs by a MnO<sub>2</sub> NS can be significantly reversed by the addition of ascorbic acid. On the basis of fluorescent recovery by ascorbic acid, we proposed a system that consisted of Hb−Fe NCs, a MnO<sub>2</sub> NS and ascorbate phosphate, and the proposed system was successfully used for alkaline phosphatase (ALP) detection in the range of 0–20 μg/mL based on the significant fluorescence recovery achieved. |
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| ISSN: | 1424-8220 |