Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis
Toxoplasmosis is a serious health problem in humans and animals resulting from obligatory intracellular invasion of reticuloendothelial tissue by Toxoplasma gondii. The profound pathologic effect of toxoplasmosis is confined to nervous tissue, but many other organs, including the liver and spleen, a...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/2165205 |
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| author | Samah Hassan Yahia Samia Elsayed Etewa Nesreen Saeed Saleh Samira Metwally Mohammad Nora Ibrahim Aboulfotouh Ahmad Mansour Kandil Mohamed Hassan Sarhan |
| author_facet | Samah Hassan Yahia Samia Elsayed Etewa Nesreen Saeed Saleh Samira Metwally Mohammad Nora Ibrahim Aboulfotouh Ahmad Mansour Kandil Mohamed Hassan Sarhan |
| author_sort | Samah Hassan Yahia |
| collection | DOAJ |
| description | Toxoplasmosis is a serious health problem in humans and animals resulting from obligatory intracellular invasion of reticuloendothelial tissue by Toxoplasma gondii. The profound pathologic effect of toxoplasmosis is confined to nervous tissue, but many other organs, including the liver and spleen, are insulted. Many molecules like caspase-3, CD3, and CD138 are implicated in the tissue immune response in a trial to alleviate hazardous toxoplasmosis impact. This study aimed to investigate the effect of chronic toxoplasmosis on the liver and spleen tissues of mice using biochemical and histopathological techniques and to detect the activity and level of expression of caspase-3, CD3, and CD138 in these tissues using immunohistochemical labeling. Compared with normal control, altered normal histological features accompanied by inflammatory reaction were recorded in hepatosplenic reticuloendothelial tissues in chronically infected mice. The biochemical profile of the liver has been changed in the form of increased liver enzymes, and oxidative stress has been evidenced by elevated nitric oxide (NO) concentration in liver homogenate. The levels of caspase3, CD3, and CD138 were markedly expressed in the liver and spleen of infected mice. Our findings revealed the persistent effect of latent toxoplasmosis on the host’s histological architecture, metabolic, and immunological profile, creating a continued challenging host-parasite relationship. |
| format | Article |
| id | doaj-art-39d0329b60c84c11a40549f3988d032e |
| institution | Kabale University |
| issn | 2090-0031 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-39d0329b60c84c11a40549f3988d032e2025-02-03T01:23:09ZengWileyJournal of Parasitology Research2090-00312022-01-01202210.1155/2022/2165205Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic ToxoplasmosisSamah Hassan Yahia0Samia Elsayed Etewa1Nesreen Saeed Saleh2Samira Metwally Mohammad3Nora Ibrahim Aboulfotouh4Ahmad Mansour Kandil5Mohamed Hassan Sarhan6Medical Parasitology DepartmentMedical Parasitology DepartmentZoology DepartmentMedical Parasitology DepartmentMedical Parasitology DepartmentPathology DepartmentMedical Parasitology DepartmentToxoplasmosis is a serious health problem in humans and animals resulting from obligatory intracellular invasion of reticuloendothelial tissue by Toxoplasma gondii. The profound pathologic effect of toxoplasmosis is confined to nervous tissue, but many other organs, including the liver and spleen, are insulted. Many molecules like caspase-3, CD3, and CD138 are implicated in the tissue immune response in a trial to alleviate hazardous toxoplasmosis impact. This study aimed to investigate the effect of chronic toxoplasmosis on the liver and spleen tissues of mice using biochemical and histopathological techniques and to detect the activity and level of expression of caspase-3, CD3, and CD138 in these tissues using immunohistochemical labeling. Compared with normal control, altered normal histological features accompanied by inflammatory reaction were recorded in hepatosplenic reticuloendothelial tissues in chronically infected mice. The biochemical profile of the liver has been changed in the form of increased liver enzymes, and oxidative stress has been evidenced by elevated nitric oxide (NO) concentration in liver homogenate. The levels of caspase3, CD3, and CD138 were markedly expressed in the liver and spleen of infected mice. Our findings revealed the persistent effect of latent toxoplasmosis on the host’s histological architecture, metabolic, and immunological profile, creating a continued challenging host-parasite relationship.http://dx.doi.org/10.1155/2022/2165205 |
| spellingShingle | Samah Hassan Yahia Samia Elsayed Etewa Nesreen Saeed Saleh Samira Metwally Mohammad Nora Ibrahim Aboulfotouh Ahmad Mansour Kandil Mohamed Hassan Sarhan Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis Journal of Parasitology Research |
| title | Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis |
| title_full | Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis |
| title_fullStr | Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis |
| title_full_unstemmed | Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis |
| title_short | Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis |
| title_sort | histopathological immunohistochemical and biochemical studies of murine hepatosplenic tissues affected by chronic toxoplasmosis |
| url | http://dx.doi.org/10.1155/2022/2165205 |
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