Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics

Antioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against DPPH•, NO•, and O2∙-...

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Main Authors: Boris M. Popović, Dubravka Štajner, Ružica Ždero, Saša Orlović, Zoran Galić
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2013/134656
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author Boris M. Popović
Dubravka Štajner
Ružica Ždero
Saša Orlović
Zoran Galić
author_facet Boris M. Popović
Dubravka Štajner
Ružica Ždero
Saša Orlović
Zoran Galić
author_sort Boris M. Popović
collection DOAJ
description Antioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against DPPH•, NO•, and O2∙- radicals, ferric reducing antioxidant power (FRAP), total phenol, tannin, flavonoid, and proanthocyanidin contents. Permanganate reducing antioxidant capacity (PRAC) was determined using water extracts. Beside, mentioned parameters, soluble proteins, lipid peroxidation (LP), pigments and proline contents were also determined. The data of different procedures were compared and analyzed by multivariate techniques (correlation matrix calculation and principal component analysis (PCA)). PCA found that investigated organs of two different oak tree species possess similar antioxidant characteristics. The superior antioxidant characteristics showed oak leaves over twigs and acorns and seem to be promising source of antioxidants with possible use in industry and pharmacy.
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institution Kabale University
issn 1537-744X
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publisher Wiley
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series The Scientific World Journal
spelling doaj-art-39c91cc056184815907ea8f60544f0672025-02-03T06:13:23ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/134656134656Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and ChemometricsBoris M. Popović0Dubravka Štajner1Ružica Ždero2Saša Orlović3Zoran Galić4Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaInstitute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, 21000 Novi Sad, SerbiaInstitute of Lowland Forestry and Environment, University of Novi Sad, Antona Čehova 13, 21000 Novi Sad, SerbiaAntioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against DPPH•, NO•, and O2∙- radicals, ferric reducing antioxidant power (FRAP), total phenol, tannin, flavonoid, and proanthocyanidin contents. Permanganate reducing antioxidant capacity (PRAC) was determined using water extracts. Beside, mentioned parameters, soluble proteins, lipid peroxidation (LP), pigments and proline contents were also determined. The data of different procedures were compared and analyzed by multivariate techniques (correlation matrix calculation and principal component analysis (PCA)). PCA found that investigated organs of two different oak tree species possess similar antioxidant characteristics. The superior antioxidant characteristics showed oak leaves over twigs and acorns and seem to be promising source of antioxidants with possible use in industry and pharmacy.http://dx.doi.org/10.1155/2013/134656
spellingShingle Boris M. Popović
Dubravka Štajner
Ružica Ždero
Saša Orlović
Zoran Galić
Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics
The Scientific World Journal
title Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics
title_full Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics
title_fullStr Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics
title_full_unstemmed Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics
title_short Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics
title_sort antioxidant characterization of oak extracts combining spectrophotometric assays and chemometrics
url http://dx.doi.org/10.1155/2013/134656
work_keys_str_mv AT borismpopovic antioxidantcharacterizationofoakextractscombiningspectrophotometricassaysandchemometrics
AT dubravkastajner antioxidantcharacterizationofoakextractscombiningspectrophotometricassaysandchemometrics
AT ruzicazdero antioxidantcharacterizationofoakextractscombiningspectrophotometricassaysandchemometrics
AT sasaorlovic antioxidantcharacterizationofoakextractscombiningspectrophotometricassaysandchemometrics
AT zorangalic antioxidantcharacterizationofoakextractscombiningspectrophotometricassaysandchemometrics