Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration Features

<italic>Goal</italic>: The early diagnosis and treatment of hepatitis is essential to reduce hepatitis-related liver function deterioration and mortality. One component of the widely-used Ishak grading system for the grading of periportal interface hepatitis is based on the percentage of...

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Main Authors: Hung-Wen Tsai, Chien-Yu Chiou, Wei-Jong Yang, Tsan-An Hsieh, Cheng-Yi Chen, Che-Wei Hsu, Yih-Jyh Lin, Min-En Hsieh, Matthew M. Yeh, Chin-Chun Chen, Meng-Ru Shen, Pau-Choo Chung
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Language:English
Published: IEEE 2024-01-01
Series:IEEE Open Journal of Engineering in Medicine and Biology
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Online Access:https://ieeexplore.ieee.org/document/10476647/
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author Hung-Wen Tsai
Chien-Yu Chiou
Wei-Jong Yang
Tsan-An Hsieh
Cheng-Yi Chen
Che-Wei Hsu
Yih-Jyh Lin
Min-En Hsieh
Matthew M. Yeh
Chin-Chun Chen
Meng-Ru Shen
Pau-Choo Chung
author_facet Hung-Wen Tsai
Chien-Yu Chiou
Wei-Jong Yang
Tsan-An Hsieh
Cheng-Yi Chen
Che-Wei Hsu
Yih-Jyh Lin
Min-En Hsieh
Matthew M. Yeh
Chin-Chun Chen
Meng-Ru Shen
Pau-Choo Chung
author_sort Hung-Wen Tsai
collection DOAJ
description <italic>Goal</italic>: The early diagnosis and treatment of hepatitis is essential to reduce hepatitis-related liver function deterioration and mortality. One component of the widely-used Ishak grading system for the grading of periportal interface hepatitis is based on the percentage of portal borders infiltrated by lymphocytes. Thus, the accurate detection of lymphocyte-infiltrated periportal regions is critical in the diagnosis of hepatitis. However, the infiltrating lymphocytes usually result in the formation of ambiguous and highly-irregular portal boundaries, and thus identifying the infiltrated portal boundary regions precisely using automated methods is challenging. This study aims to develop a deep-learning-based automatic detection framework to assist diagnosis. <italic>Methods</italic>: The present study proposes a framework consisting of a Structurally-REfined Deep Portal Segmentation module and an Infiltrated Periportal Region Detection module based on heterogeneous infiltration features to accurately identify the infiltrated periportal regions in liver Whole Slide Images. <italic>Results</italic>: The proposed method achieves 0.725 in F1-score of lymphocyte-infiltrated periportal region detection. Moreover, the statistics of the ratio of the detected infiltrated portal boundary have high correlation to the Ishak grade (Spearman&#x0027;s correlations more than 0.87 with p-values less than 0.001) and medium correlation to the liver function index aspartate aminotransferase and alanine aminotransferase (Spearman&#x0027;s correlations more than 0.63 and 0.57 with p-values less than 0.001). <italic>Conclusions</italic>: The study shows the statistics of the ratio of infiltrated portal boundary have correlation to the Ishak grade and liver function index. The proposed framework provides pathologists with a useful and reliable tool for hepatitis diagnosis.
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spelling doaj-art-39b885ccbc2b47bab366ff030aa2c10c2025-08-20T03:33:11ZengIEEEIEEE Open Journal of Engineering in Medicine and Biology2644-12762024-01-01526127010.1109/OJEMB.2024.337947910476647Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration FeaturesHung-Wen Tsai0https://orcid.org/0000-0001-9223-2535Chien-Yu Chiou1https://orcid.org/0000-0002-6737-2963Wei-Jong Yang2https://orcid.org/0000-0002-4738-4617Tsan-An Hsieh3https://orcid.org/0009-0007-3694-0917Cheng-Yi Chen4Che-Wei Hsu5https://orcid.org/0000-0002-6742-9835Yih-Jyh Lin6https://orcid.org/0000-0003-0998-3229Min-En Hsieh7https://orcid.org/0009-0001-9703-8456Matthew M. Yeh8Chin-Chun Chen9https://orcid.org/0009-0006-4604-2961Meng-Ru Shen10https://orcid.org/0000-0003-0506-3505Pau-Choo Chung11https://orcid.org/0000-0002-8660-570XDepartment of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Electrical Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Artificial Intelligence and Computer Engineering, National Chin-Yi University of Technology, Taichung, TaiwanInstitute of Computer and Communication Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Electrical Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, WA, USADepartment of Statistics, National Cheng Kung University, Tainan, TaiwanDepartment of Pharmacology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Electrical Engineering, National Cheng Kung University, Tainan, Taiwan<italic>Goal</italic>: The early diagnosis and treatment of hepatitis is essential to reduce hepatitis-related liver function deterioration and mortality. One component of the widely-used Ishak grading system for the grading of periportal interface hepatitis is based on the percentage of portal borders infiltrated by lymphocytes. Thus, the accurate detection of lymphocyte-infiltrated periportal regions is critical in the diagnosis of hepatitis. However, the infiltrating lymphocytes usually result in the formation of ambiguous and highly-irregular portal boundaries, and thus identifying the infiltrated portal boundary regions precisely using automated methods is challenging. This study aims to develop a deep-learning-based automatic detection framework to assist diagnosis. <italic>Methods</italic>: The present study proposes a framework consisting of a Structurally-REfined Deep Portal Segmentation module and an Infiltrated Periportal Region Detection module based on heterogeneous infiltration features to accurately identify the infiltrated periportal regions in liver Whole Slide Images. <italic>Results</italic>: The proposed method achieves 0.725 in F1-score of lymphocyte-infiltrated periportal region detection. Moreover, the statistics of the ratio of the detected infiltrated portal boundary have high correlation to the Ishak grade (Spearman&#x0027;s correlations more than 0.87 with p-values less than 0.001) and medium correlation to the liver function index aspartate aminotransferase and alanine aminotransferase (Spearman&#x0027;s correlations more than 0.63 and 0.57 with p-values less than 0.001). <italic>Conclusions</italic>: The study shows the statistics of the ratio of infiltrated portal boundary have correlation to the Ishak grade and liver function index. The proposed framework provides pathologists with a useful and reliable tool for hepatitis diagnosis.https://ieeexplore.ieee.org/document/10476647/Deep learningheterogeneous featuresinterface hepatitisIshak gradingstructural analysis
spellingShingle Hung-Wen Tsai
Chien-Yu Chiou
Wei-Jong Yang
Tsan-An Hsieh
Cheng-Yi Chen
Che-Wei Hsu
Yih-Jyh Lin
Min-En Hsieh
Matthew M. Yeh
Chin-Chun Chen
Meng-Ru Shen
Pau-Choo Chung
Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration Features
IEEE Open Journal of Engineering in Medicine and Biology
Deep learning
heterogeneous features
interface hepatitis
Ishak grading
structural analysis
title Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration Features
title_full Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration Features
title_fullStr Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration Features
title_full_unstemmed Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration Features
title_short Lymphocyte-Infiltrated Periportal Region Detection With Structurally-Refined Deep Portal Segmentation and Heterogeneous Infiltration Features
title_sort lymphocyte infiltrated periportal region detection with structurally refined deep portal segmentation and heterogeneous infiltration features
topic Deep learning
heterogeneous features
interface hepatitis
Ishak grading
structural analysis
url https://ieeexplore.ieee.org/document/10476647/
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