Mesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritis
Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by joint inflammation, leading to pain, swelling, stiffness, and joint damage. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) hold significant promise as therapeutic agents owing to their potent paracrine activi...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2024-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400030G.pdf |
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| author | Guo Zhonghua He Mengyuan Shi Dongliang Zhang Zhongbo Wang Lisha Ren Bowen Wang Yunfei Wang Junjie Yang Shaoxiang Yu Hongchao |
| author_facet | Guo Zhonghua He Mengyuan Shi Dongliang Zhang Zhongbo Wang Lisha Ren Bowen Wang Yunfei Wang Junjie Yang Shaoxiang Yu Hongchao |
| author_sort | Guo Zhonghua |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by joint inflammation, leading to pain, swelling, stiffness, and joint damage. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) hold significant promise as therapeutic agents owing to their potent paracrine activities. This study investigated the anti-inflammatory effects of human umbilical cord-derived MSC (hUCSC)-EVs overexpressing miR-124 in collagen-induced arthritis (CIA). hUCSCs were transfected with miR-124, and hUCSC-EVs were isolated and characterized. Arthritis was induced in mice by collagen injection, followed by intravenous administration of miR-124 EVs, miR-control, or vehicle. Cytokine levels, including interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, IL-10, and transforming growth factor (TGF)-β, were measured in the joints using real-time PCR by enzyme-linked immunosorbent assay (ELISA). miR-124-hUCSC-EVs showed enrichment of miR-124. In arthritic mice, intravenous treatment with miR-124 EVs significantly reduced the clinical score, increased anti-inflammatory cytokines IL-10 and TGF-β, and lowered inflammatory cytokines IL-6, TNF-α, and IL-1β in the joints. This study shows that miR-124 EVs have anti-inflammatory activity in arthritis by suppressing pro-inflammatory cytokines through miR-124 overexpression. MSC-EVs overexpressed with inflammation-modulating miRNAs offer a promising cell-free approach for treating inflammatory arthritis. |
| format | Article |
| id | doaj-art-39abe22ee5d740c3a639ac94f6224307 |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj-art-39abe22ee5d740c3a639ac94f62243072025-01-30T06:38:39ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176439940710.2298/ABS240630030G0354-46642400030GMesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritisGuo Zhonghua0https://orcid.org/0000-0002-9606-752XHe Mengyuan1https://orcid.org/0009-0003-0651-2808Shi Dongliang2https://orcid.org/0009-0003-1659-8772Zhang Zhongbo3https://orcid.org/0009-0007-2908-389XWang Lisha4https://orcid.org/0009-0002-9696-4308Ren Bowen5https://orcid.org/0009-0005-6141-8392Wang Yunfei6https://orcid.org/0009-0002-0737-5588Wang Junjie7https://orcid.org/0009-0007-3182-3869Yang Shaoxiang8https://orcid.org/0009-0005-8334-3977Yu Hongchao9Department of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaHenan University of Traditional Chinese Medicine, Zhengzhou City, Henan Province, China, Graduate studentDepartment of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaDepartment of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaDepartment of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaDepartment of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaDepartment of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaDepartment of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaDepartment of Orthopedics, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou City, Henan Province, ChinaDepartment of Orthopedics, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang City, Shaanxi Province, ChinaRheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by joint inflammation, leading to pain, swelling, stiffness, and joint damage. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) hold significant promise as therapeutic agents owing to their potent paracrine activities. This study investigated the anti-inflammatory effects of human umbilical cord-derived MSC (hUCSC)-EVs overexpressing miR-124 in collagen-induced arthritis (CIA). hUCSCs were transfected with miR-124, and hUCSC-EVs were isolated and characterized. Arthritis was induced in mice by collagen injection, followed by intravenous administration of miR-124 EVs, miR-control, or vehicle. Cytokine levels, including interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, IL-10, and transforming growth factor (TGF)-β, were measured in the joints using real-time PCR by enzyme-linked immunosorbent assay (ELISA). miR-124-hUCSC-EVs showed enrichment of miR-124. In arthritic mice, intravenous treatment with miR-124 EVs significantly reduced the clinical score, increased anti-inflammatory cytokines IL-10 and TGF-β, and lowered inflammatory cytokines IL-6, TNF-α, and IL-1β in the joints. This study shows that miR-124 EVs have anti-inflammatory activity in arthritis by suppressing pro-inflammatory cytokines through miR-124 overexpression. MSC-EVs overexpressed with inflammation-modulating miRNAs offer a promising cell-free approach for treating inflammatory arthritis.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400030G.pdfmesenchymal stem cellsextracellular vesiclesexosomearthritiscytokine |
| spellingShingle | Guo Zhonghua He Mengyuan Shi Dongliang Zhang Zhongbo Wang Lisha Ren Bowen Wang Yunfei Wang Junjie Yang Shaoxiang Yu Hongchao Mesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritis Archives of Biological Sciences mesenchymal stem cells extracellular vesicles exosome arthritis cytokine |
| title | Mesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritis |
| title_full | Mesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritis |
| title_fullStr | Mesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritis |
| title_full_unstemmed | Mesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritis |
| title_short | Mesenchymal stem cell-derived extracellular vesicles enriched with miR-124 exhibit anti-inflammatory effects in collagen-induced arthritis |
| title_sort | mesenchymal stem cell derived extracellular vesicles enriched with mir 124 exhibit anti inflammatory effects in collagen induced arthritis |
| topic | mesenchymal stem cells extracellular vesicles exosome arthritis cytokine |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400030G.pdf |
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