Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1

From December 2022 to January 2023, SARS-CoV-2 infections caused by BA.5 and BF.7 subvariants of B.1.1.529 (Omicron) spread in China. It is urgently needed to evaluate the protective immune responses in the infected individuals against the current circulating variants to predict the future potential...

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Main Authors: Shuo Liu, Ziteng Liang, Jianhui Nie, Wei bo Gao, Xinyi Li, Li Zhang, Yuanling Yu, Youchun Wang, Weijin Huang
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Emerging Microbes and Infections
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Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2023.2225638
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author Shuo Liu
Ziteng Liang
Jianhui Nie
Wei bo Gao
Xinyi Li
Li Zhang
Yuanling Yu
Youchun Wang
Weijin Huang
author_facet Shuo Liu
Ziteng Liang
Jianhui Nie
Wei bo Gao
Xinyi Li
Li Zhang
Yuanling Yu
Youchun Wang
Weijin Huang
author_sort Shuo Liu
collection DOAJ
description From December 2022 to January 2023, SARS-CoV-2 infections caused by BA.5 and BF.7 subvariants of B.1.1.529 (Omicron) spread in China. It is urgently needed to evaluate the protective immune responses in the infected individuals against the current circulating variants to predict the future potential infection waves, such as the BQ.1.1, XBB.1.5, and CH1.1 variants. In this study, we constructed a panel of pseudotyped viruses for SARS-CoV-2 for the past and current circulating variants, including D614G, Delta, BA.1, BA.5, BF.7, BQ.1.1, XBB.1.5 and CH.1.1. We investigated the neutralization sensitivity of these pseudotyped viruses to sera from individuals who had BA.5 or BF.7 breakthrough infections in the infection wave of last December in China. The mean neutralization ID50 against infected variants BA.5 and BF.7 are 533 and 444, respectively. The highest neutralizing antibody level was observed when tested against the D614G strain, with the ID50 of 742, which is about 1.52-folds higher than that against the BA.5/BF.7 variant. The ID50 for BA.1, Delta, and BQ.1.1 pseudotyped viruses were about 2–3 folds lower when compared to BA.5/BF.7. The neutralization activities of these serum samples against XBB.1.5 and CH.1.1 decreased 7.39-folds and 15.25-folds when compared to that against BA.5/BF.7. The immune escape capacity of these two variants might predict new infection waves in future when the neutralizing antibody levels decrease furtherly.
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spelling doaj-art-399c70797b084abca6f53d659c6e15db2025-08-20T02:37:28ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112210.1080/22221751.2023.2225638Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1Shuo Liu0Ziteng Liang1Jianhui Nie2Wei bo Gao3Xinyi Li4Li Zhang5Yuanling Yu6Youchun Wang7Weijin Huang8Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of ChinaChinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of ChinaDivision of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing, People’s Republic of ChinaDepartment of Emergency, Peking University People’s Hospital, Beijing, People’s Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, People’s Republic of ChinaDivision of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing, People’s Republic of ChinaChangping Laboratory, Beijing, People’s Republic of ChinaChinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of ChinaDivision of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing, People’s Republic of ChinaFrom December 2022 to January 2023, SARS-CoV-2 infections caused by BA.5 and BF.7 subvariants of B.1.1.529 (Omicron) spread in China. It is urgently needed to evaluate the protective immune responses in the infected individuals against the current circulating variants to predict the future potential infection waves, such as the BQ.1.1, XBB.1.5, and CH1.1 variants. In this study, we constructed a panel of pseudotyped viruses for SARS-CoV-2 for the past and current circulating variants, including D614G, Delta, BA.1, BA.5, BF.7, BQ.1.1, XBB.1.5 and CH.1.1. We investigated the neutralization sensitivity of these pseudotyped viruses to sera from individuals who had BA.5 or BF.7 breakthrough infections in the infection wave of last December in China. The mean neutralization ID50 against infected variants BA.5 and BF.7 are 533 and 444, respectively. The highest neutralizing antibody level was observed when tested against the D614G strain, with the ID50 of 742, which is about 1.52-folds higher than that against the BA.5/BF.7 variant. The ID50 for BA.1, Delta, and BQ.1.1 pseudotyped viruses were about 2–3 folds lower when compared to BA.5/BF.7. The neutralization activities of these serum samples against XBB.1.5 and CH.1.1 decreased 7.39-folds and 15.25-folds when compared to that against BA.5/BF.7. The immune escape capacity of these two variants might predict new infection waves in future when the neutralizing antibody levels decrease furtherly.https://www.tandfonline.com/doi/10.1080/22221751.2023.2225638SARS-CoV-2neutralizing antibodybreakthrough infectionconvalescence serumBA.5/BF.7
spellingShingle Shuo Liu
Ziteng Liang
Jianhui Nie
Wei bo Gao
Xinyi Li
Li Zhang
Yuanling Yu
Youchun Wang
Weijin Huang
Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1
Emerging Microbes and Infections
SARS-CoV-2
neutralizing antibody
breakthrough infection
convalescence serum
BA.5/BF.7
title Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1
title_full Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1
title_fullStr Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1
title_full_unstemmed Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1
title_short Sera from breakthrough infections with SARS-CoV-2 BA.5 or BF.7 showed lower neutralization activity against XBB.1.5 and CH.1.1
title_sort sera from breakthrough infections with sars cov 2 ba 5 or bf 7 showed lower neutralization activity against xbb 1 5 and ch 1 1
topic SARS-CoV-2
neutralizing antibody
breakthrough infection
convalescence serum
BA.5/BF.7
url https://www.tandfonline.com/doi/10.1080/22221751.2023.2225638
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