Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples
This study aims at providing recommendations concerning the validation of analytical protocols by using routine samples. It is intended to provide a case-study on how to validate the analytical methods in different environmental matrices. In order to analyze the selected compounds (pesticides and po...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/9796457 |
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| author | Oscar Pindado Jiménez Susana García Alonso Rosa María Pérez Pastor |
| author_facet | Oscar Pindado Jiménez Susana García Alonso Rosa María Pérez Pastor |
| author_sort | Oscar Pindado Jiménez |
| collection | DOAJ |
| description | This study aims at providing recommendations concerning the validation of analytical protocols by using routine samples. It is intended to provide a case-study on how to validate the analytical methods in different environmental matrices. In order to analyze the selected compounds (pesticides and polychlorinated biphenyls) in two different environmental matrices, the current work has performed and validated two analytical procedures by GC-MS. A description is given of the validation of the two protocols by the analysis of more than 30 samples of water and sediments collected along nine months. The present work also scopes the uncertainty associated with both analytical protocols. In detail, uncertainty of water sample was performed through a conventional approach. However, for the sediments matrices, the estimation of proportional/constant bias is also included due to its inhomogeneity. Results for the sediment matrix are reliable, showing a range 25–35% of analytical variability associated with intermediate conditions. The analytical methodology for the water matrix determines the selected compounds with acceptable recoveries and the combined uncertainty ranges between 20 and 30%. Analyzing routine samples is rarely applied to assess trueness of novel analytical methods and up to now this methodology was not focused on organochlorine compounds in environmental matrices. |
| format | Article |
| id | doaj-art-399a118258934c20bdbcdd26d8df71fc |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-399a118258934c20bdbcdd26d8df71fc2025-02-03T01:28:51ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/97964579796457Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine SamplesOscar Pindado Jiménez0Susana García Alonso1Rosa María Pérez Pastor2Chemistry Division, CIEMAT, Av. Complutense 40, 28040 Madrid, SpainChemistry Division, CIEMAT, Av. Complutense 40, 28040 Madrid, SpainChemistry Division, CIEMAT, Av. Complutense 40, 28040 Madrid, SpainThis study aims at providing recommendations concerning the validation of analytical protocols by using routine samples. It is intended to provide a case-study on how to validate the analytical methods in different environmental matrices. In order to analyze the selected compounds (pesticides and polychlorinated biphenyls) in two different environmental matrices, the current work has performed and validated two analytical procedures by GC-MS. A description is given of the validation of the two protocols by the analysis of more than 30 samples of water and sediments collected along nine months. The present work also scopes the uncertainty associated with both analytical protocols. In detail, uncertainty of water sample was performed through a conventional approach. However, for the sediments matrices, the estimation of proportional/constant bias is also included due to its inhomogeneity. Results for the sediment matrix are reliable, showing a range 25–35% of analytical variability associated with intermediate conditions. The analytical methodology for the water matrix determines the selected compounds with acceptable recoveries and the combined uncertainty ranges between 20 and 30%. Analyzing routine samples is rarely applied to assess trueness of novel analytical methods and up to now this methodology was not focused on organochlorine compounds in environmental matrices.http://dx.doi.org/10.1155/2017/9796457 |
| spellingShingle | Oscar Pindado Jiménez Susana García Alonso Rosa María Pérez Pastor Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples Journal of Analytical Methods in Chemistry |
| title | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
| title_full | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
| title_fullStr | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
| title_full_unstemmed | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
| title_short | Validating Analytical Protocols to Determine Selected Pesticides and PCBs Using Routine Samples |
| title_sort | validating analytical protocols to determine selected pesticides and pcbs using routine samples |
| url | http://dx.doi.org/10.1155/2017/9796457 |
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