Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats
p56lck is involved in the maturation of T-cells from double negative (DN) into double positive (DP) T-cells. The objective of this experiment was to determine changes in the levels of thymic and splenic T-cell p56lck using Western immunoblotting, a...
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| Format: | Article |
| Language: | English |
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Wiley
2005-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520400017393 |
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| author | Heather J. Hosea Edward S. Rector Carla G. Taylor |
| author_facet | Heather J. Hosea Edward S. Rector Carla G. Taylor |
| author_sort | Heather J. Hosea |
| collection | DOAJ |
| description | p56lck is involved in the maturation of T-cells from double negative
(DN) into double positive (DP) T-cells. The objective of this experiment was to
determine changes in the levels of thymic and splenic T-cell
p56lck using Western
immunoblotting, along with the proportion and number of T-cell subsets in thymus,
spleen and blood using flow cytometry in growing Sprague-Dawley rats. Thymic
p56lck levels were negatively correlated with age
(r=-0.42,
p=0.04) and positively
correlated with age in the spleen (r=0.50, p=0.01).
Nine-week-old rats had
a higher percentage of thymic DN and CD8 cells with fewer DP cells compared
to younger rats. There were minor differences in the proportions of T-cell subsets
in the spleen and blood. T-cell numbers remained proportional to body weight in
the lymphoid organs; however, the lower absolute number of
T-cells in the younger rats might indicate that they are less able to respond to antigens. |
| format | Article |
| id | doaj-art-3986d5e583464fd28a65fc95d3451763 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-3986d5e583464fd28a65fc95d34517632025-02-03T06:42:28ZengWileyClinical and Developmental Immunology1740-25221740-25302005-01-01121758410.1080/17402520400017393Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young RatsHeather J. Hosea0Edward S. Rector1Carla G. Taylor2Departments of Human Nutritional Sciences, University of Manitoba, 190 Dysart Road, Winnipeg, MB R3T 2N2, CanadaDepartment of Immunology, University of Manitoba, Winnipeg, MB R3E 0W3, CanadaDepartments of Human Nutritional Sciences, University of Manitoba, 190 Dysart Road, Winnipeg, MB R3T 2N2, Canadap56lck is involved in the maturation of T-cells from double negative (DN) into double positive (DP) T-cells. The objective of this experiment was to determine changes in the levels of thymic and splenic T-cell p56lck using Western immunoblotting, along with the proportion and number of T-cell subsets in thymus, spleen and blood using flow cytometry in growing Sprague-Dawley rats. Thymic p56lck levels were negatively correlated with age (r=-0.42, p=0.04) and positively correlated with age in the spleen (r=0.50, p=0.01). Nine-week-old rats had a higher percentage of thymic DN and CD8 cells with fewer DP cells compared to younger rats. There were minor differences in the proportions of T-cell subsets in the spleen and blood. T-cell numbers remained proportional to body weight in the lymphoid organs; however, the lower absolute number of T-cells in the younger rats might indicate that they are less able to respond to antigens.http://dx.doi.org/10.1080/17402520400017393 |
| spellingShingle | Heather J. Hosea Edward S. Rector Carla G. Taylor Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats Clinical and Developmental Immunology |
| title | Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats |
| title_full | Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats |
| title_fullStr | Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats |
| title_full_unstemmed | Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats |
| title_short | Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats |
| title_sort | age related changes in p56lck protein levels and phenotypic distribution of t lymphocytes in young rats |
| url | http://dx.doi.org/10.1080/17402520400017393 |
| work_keys_str_mv | AT heatherjhosea agerelatedchangesinp56lckproteinlevelsandphenotypicdistributionoftlymphocytesinyoungrats AT edwardsrector agerelatedchangesinp56lckproteinlevelsandphenotypicdistributionoftlymphocytesinyoungrats AT carlagtaylor agerelatedchangesinp56lckproteinlevelsandphenotypicdistributionoftlymphocytesinyoungrats |