Age-related Changes in p56lck Protein Levels and Phenotypic Distribution of T Lymphocytes in Young Rats
p56lck is involved in the maturation of T-cells from double negative (DN) into double positive (DP) T-cells. The objective of this experiment was to determine changes in the levels of thymic and splenic T-cell p56lck using Western immunoblotting, a...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520400017393 |
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| Summary: | p56lck is involved in the maturation of T-cells from double negative
(DN) into double positive (DP) T-cells. The objective of this experiment was to
determine changes in the levels of thymic and splenic T-cell
p56lck using Western
immunoblotting, along with the proportion and number of T-cell subsets in thymus,
spleen and blood using flow cytometry in growing Sprague-Dawley rats. Thymic
p56lck levels were negatively correlated with age
(r=-0.42,
p=0.04) and positively
correlated with age in the spleen (r=0.50, p=0.01).
Nine-week-old rats had
a higher percentage of thymic DN and CD8 cells with fewer DP cells compared
to younger rats. There were minor differences in the proportions of T-cell subsets
in the spleen and blood. T-cell numbers remained proportional to body weight in
the lymphoid organs; however, the lower absolute number of
T-cells in the younger rats might indicate that they are less able to respond to antigens. |
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| ISSN: | 1740-2522 1740-2530 |