3D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fascicules
Treating peripheral nerve injury (PNI) is a prevalent clinical challenge. The improper dispersion of regenerating axons makes it difficult to develop nerve guidance conduits (NGCs) for treating PNI. The multichannel NGCs, designed to mimic the fascicular structure of nerves, are proposed as an alter...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000729 |
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| author | Woo-Youl Maeng Yerim Lee Szu-Han Chen Kyung Su Kim Daeun Sung Wan-Ling Tseng Gyu-Nam Kim Young-Hag Koh Yuan-Yu Hsueh Jahyun Koo |
| author_facet | Woo-Youl Maeng Yerim Lee Szu-Han Chen Kyung Su Kim Daeun Sung Wan-Ling Tseng Gyu-Nam Kim Young-Hag Koh Yuan-Yu Hsueh Jahyun Koo |
| author_sort | Woo-Youl Maeng |
| collection | DOAJ |
| description | Treating peripheral nerve injury (PNI) is a prevalent clinical challenge. The improper dispersion of regenerating axons makes it difficult to develop nerve guidance conduits (NGCs) for treating PNI. The multichannel NGCs, designed to mimic the fascicular structure of nerves, are proposed as an alternative to single hollow lumen NGCs. Hydrogel-based NGCs with microscale multichannels resembling actual nerve fascicles are fabricated using digital light processing as 3D printing. Gelatin methacryloyl (GelMA) and polyethylene glycol diacrylate (PEGDA), which are biodegradable and photocurable, are used as the printing solution. The addition of a food-grade dye to the printing solution can prevent overcuring by adjusting the optical path length of light and regulating the polymerization rate. This work further demonstrates that the addition of dyes can enable high-resolution printing, resulting in the achievement of fine multichannels with a diameter of 200 μm. In vivo animal studies using a rat sciatic nerve gap model show that GelMA/PEGDA-based multichannel NGCs can significantly improve peripheral nerve regeneration, as indicated by improved paw sensory recoveries, increased hindlimb gait function, and muscle fiber regeneration. Furthermore, the mechanical properties, pore size, and biodegradation rate of the hydrogel constituting the NGCs successfully demonstrate the feasibility of hydrogel-based multichannel NGCs for accelerating neurologic recoveries. |
| format | Article |
| id | doaj-art-397c27605c4c43b7b804ae4b240d98b8 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-397c27605c4c43b7b804ae4b240d98b82025-01-30T05:14:50ZengElsevierMaterials Today Bio2590-00642025-04-01311015143D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fasciculesWoo-Youl Maeng0Yerim Lee1Szu-Han Chen2Kyung Su Kim3Daeun Sung4Wan-Ling Tseng5Gyu-Nam Kim6Young-Hag Koh7Yuan-Yu Hsueh8Jahyun Koo9School of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, 60208, USA; Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, 60208, USASchool of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea; Interdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of KoreaDivision of Plastic and Reconstructive Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 70456, TaiwanSchool of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea; Interdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of KoreaSchool of Biomedical Engineering, Korea University, Seoul, 02841, Republic of KoreaDivision of Plastic and Reconstructive Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 70456, TaiwanSchool of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea; Interdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of KoreaSchool of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea; Interdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of KoreaDivision of Plastic and Reconstructive Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 70456, Taiwan; Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, 701, Taiwan; Corresponding author. Division of Plastic and Reconstructive Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 70456, Taiwan.School of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea; Interdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of Korea; Corresponding author. School of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea.Treating peripheral nerve injury (PNI) is a prevalent clinical challenge. The improper dispersion of regenerating axons makes it difficult to develop nerve guidance conduits (NGCs) for treating PNI. The multichannel NGCs, designed to mimic the fascicular structure of nerves, are proposed as an alternative to single hollow lumen NGCs. Hydrogel-based NGCs with microscale multichannels resembling actual nerve fascicles are fabricated using digital light processing as 3D printing. Gelatin methacryloyl (GelMA) and polyethylene glycol diacrylate (PEGDA), which are biodegradable and photocurable, are used as the printing solution. The addition of a food-grade dye to the printing solution can prevent overcuring by adjusting the optical path length of light and regulating the polymerization rate. This work further demonstrates that the addition of dyes can enable high-resolution printing, resulting in the achievement of fine multichannels with a diameter of 200 μm. In vivo animal studies using a rat sciatic nerve gap model show that GelMA/PEGDA-based multichannel NGCs can significantly improve peripheral nerve regeneration, as indicated by improved paw sensory recoveries, increased hindlimb gait function, and muscle fiber regeneration. Furthermore, the mechanical properties, pore size, and biodegradation rate of the hydrogel constituting the NGCs successfully demonstrate the feasibility of hydrogel-based multichannel NGCs for accelerating neurologic recoveries.http://www.sciencedirect.com/science/article/pii/S2590006425000729Nerve guidance conduitsPeripheral nerve injuryMimicking real nerve fasciclesVAT-Free DLPIn vivo animal studiesFood-grade dye |
| spellingShingle | Woo-Youl Maeng Yerim Lee Szu-Han Chen Kyung Su Kim Daeun Sung Wan-Ling Tseng Gyu-Nam Kim Young-Hag Koh Yuan-Yu Hsueh Jahyun Koo 3D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fascicules Materials Today Bio Nerve guidance conduits Peripheral nerve injury Mimicking real nerve fascicles VAT-Free DLP In vivo animal studies Food-grade dye |
| title | 3D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fascicules |
| title_full | 3D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fascicules |
| title_fullStr | 3D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fascicules |
| title_full_unstemmed | 3D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fascicules |
| title_short | 3D printed biodegradable hydrogel-based multichannel nerve conduits mimicking peripheral nerve fascicules |
| title_sort | 3d printed biodegradable hydrogel based multichannel nerve conduits mimicking peripheral nerve fascicules |
| topic | Nerve guidance conduits Peripheral nerve injury Mimicking real nerve fascicles VAT-Free DLP In vivo animal studies Food-grade dye |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000729 |
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