A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisition
Tick-borne diseases are a growing public health concern in the United States, with cases rising steadily each year. Lyme borreliosis, or Lyme disease, remains the most prevalent, affecting approximately 476,000 individuals annually. Human-driven changes in climate and ecosystems have expanded the ha...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1527821/full |
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| author | Aaron Scholl Bingjie Li John Dennis Sandip De |
| author_facet | Aaron Scholl Bingjie Li John Dennis Sandip De |
| author_sort | Aaron Scholl |
| collection | DOAJ |
| description | Tick-borne diseases are a growing public health concern in the United States, with cases rising steadily each year. Lyme borreliosis, or Lyme disease, remains the most prevalent, affecting approximately 476,000 individuals annually. Human-driven changes in climate and ecosystems have expanded the habitat of pathogen-carrying ticks, facilitating the spread of these infections. Additionally, increased instances of tick-borne diseases transmission through human tissues have been reported. Despite ongoing efforts to manage these infections, their incidence continues to rise. To develop effective control measures against these diseases and prevent the transmission of tick-borne infections through human and animal tissues, it is very important to develop detection assays and understand the transmission mechanisms of tick-borne infections. In this study, we provide detailed descriptions and visual references for larval and nymphal tick feeding on mice, focusing on the transmission and acquisition of Borrelia burgdorferi (sensu stricto). These methodologies can be applied to study other tick-borne diseases, tick vectorial capacity, and tick biology, aiding in the development of detection strategies to combat these infections. |
| format | Article |
| id | doaj-art-3971a1ae1b0f406c98aebc72873793dc |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-3971a1ae1b0f406c98aebc72873793dc2025-02-05T07:32:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15278211527821A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisitionAaron Scholl0Bingjie Li1John Dennis2Sandip De3Tumor Vaccines and Biotechnology Branch, Division of Cellular Therapy 2, Office of Cellular Therapy and Human Tissue, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, United StatesTumor Vaccines and Biotechnology Branch, Division of Cellular Therapy 2, Office of Cellular Therapy and Human Tissue, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, United StatesDivision of Veterinary Services, Office of Management, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, United StatesTumor Vaccines and Biotechnology Branch, Division of Cellular Therapy 2, Office of Cellular Therapy and Human Tissue, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, United StatesTick-borne diseases are a growing public health concern in the United States, with cases rising steadily each year. Lyme borreliosis, or Lyme disease, remains the most prevalent, affecting approximately 476,000 individuals annually. Human-driven changes in climate and ecosystems have expanded the habitat of pathogen-carrying ticks, facilitating the spread of these infections. Additionally, increased instances of tick-borne diseases transmission through human tissues have been reported. Despite ongoing efforts to manage these infections, their incidence continues to rise. To develop effective control measures against these diseases and prevent the transmission of tick-borne infections through human and animal tissues, it is very important to develop detection assays and understand the transmission mechanisms of tick-borne infections. In this study, we provide detailed descriptions and visual references for larval and nymphal tick feeding on mice, focusing on the transmission and acquisition of Borrelia burgdorferi (sensu stricto). These methodologies can be applied to study other tick-borne diseases, tick vectorial capacity, and tick biology, aiding in the development of detection strategies to combat these infections.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1527821/fulltickLymeBorrelia burgdorferimicefeedingtransmission |
| spellingShingle | Aaron Scholl Bingjie Li John Dennis Sandip De A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisition Frontiers in Microbiology tick Lyme Borrelia burgdorferi mice feeding transmission |
| title | A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisition |
| title_full | A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisition |
| title_fullStr | A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisition |
| title_full_unstemmed | A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisition |
| title_short | A comprehensive method on black-legged tick larvae and nymph feeding on mice to study Lyme disease transmission and acquisition |
| title_sort | comprehensive method on black legged tick larvae and nymph feeding on mice to study lyme disease transmission and acquisition |
| topic | tick Lyme Borrelia burgdorferi mice feeding transmission |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1527821/full |
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