Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh
Adipose-derived stem cell (ADSC) is considered as a cell source potentially useful for angiogenesis in tissue engineering and regenerative medicine. This study investigated the growth and endothelial differentiation of human ADSCs on polyglycolic acid/polylactic acid (PGA/PLA) mesh compared to 2D pl...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/350718 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564944600563712 |
|---|---|
| author | Meng Deng Yunpeng Gu Zhenjun Liu Yue Qi Gui E. Ma Ning Kang |
| author_facet | Meng Deng Yunpeng Gu Zhenjun Liu Yue Qi Gui E. Ma Ning Kang |
| author_sort | Meng Deng |
| collection | DOAJ |
| description | Adipose-derived stem cell (ADSC) is considered as a cell source potentially useful for angiogenesis in tissue engineering and regenerative medicine. This study investigated the growth and endothelial differentiation of human ADSCs on polyglycolic acid/polylactic acid (PGA/PLA) mesh compared to 2D plastic. Cell adhesion, viability, and distribution of hADSCs on PGA/PLA mesh were observed by CM-Dil labeling, live/dead staining, and SEM examination while endothelial differentiation was evaluated by flow cytometry, Ac-LDL/UEA-1 uptake assay, immunofluorescence stainings, and gene expression analysis of endothelial related markers. Results showed hADSCs gained a mature endothelial phenotype with a positive ratio of 21.4 ± 3.7% for CD31+/CD34− when induced in 3D mesh after 21 days, which was further verified by the expressions of a comprehensive range of endothelial related markers, whereas hADSCs in 2D induced and 2D/3D noninduced groups all failed to differentiate into endothelial cells. Moreover, compared to 2D groups, the expression for α-SMA was markedly suppressed in 3D cultured hADSCs. This study first demonstrated the endothelial differentiation of hADSCs on the PGA/PLA mesh and pointed out the synergistic effect of PGA/PLA 3D culture and growth factors on the acquisition of mature characteristic endothelial phenotype. We believed this study would be the initial step towards the generation of prevascularized tissue engineered constructs. |
| format | Article |
| id | doaj-art-395eb0eee18f4ddda7b62e513f621494 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-395eb0eee18f4ddda7b62e513f6214942025-02-03T01:09:44ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/350718350718Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid MeshMeng Deng0Yunpeng Gu1Zhenjun Liu2Yue Qi3Gui E. Ma4Ning Kang5Liposuction Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, ChinaLiposuction Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, ChinaLiposuction Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, ChinaLiposuction Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, ChinaLiposuction Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, ChinaResearch Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, ChinaAdipose-derived stem cell (ADSC) is considered as a cell source potentially useful for angiogenesis in tissue engineering and regenerative medicine. This study investigated the growth and endothelial differentiation of human ADSCs on polyglycolic acid/polylactic acid (PGA/PLA) mesh compared to 2D plastic. Cell adhesion, viability, and distribution of hADSCs on PGA/PLA mesh were observed by CM-Dil labeling, live/dead staining, and SEM examination while endothelial differentiation was evaluated by flow cytometry, Ac-LDL/UEA-1 uptake assay, immunofluorescence stainings, and gene expression analysis of endothelial related markers. Results showed hADSCs gained a mature endothelial phenotype with a positive ratio of 21.4 ± 3.7% for CD31+/CD34− when induced in 3D mesh after 21 days, which was further verified by the expressions of a comprehensive range of endothelial related markers, whereas hADSCs in 2D induced and 2D/3D noninduced groups all failed to differentiate into endothelial cells. Moreover, compared to 2D groups, the expression for α-SMA was markedly suppressed in 3D cultured hADSCs. This study first demonstrated the endothelial differentiation of hADSCs on the PGA/PLA mesh and pointed out the synergistic effect of PGA/PLA 3D culture and growth factors on the acquisition of mature characteristic endothelial phenotype. We believed this study would be the initial step towards the generation of prevascularized tissue engineered constructs.http://dx.doi.org/10.1155/2015/350718 |
| spellingShingle | Meng Deng Yunpeng Gu Zhenjun Liu Yue Qi Gui E. Ma Ning Kang Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh Stem Cells International |
| title | Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh |
| title_full | Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh |
| title_fullStr | Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh |
| title_full_unstemmed | Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh |
| title_short | Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh |
| title_sort | endothelial differentiation of human adipose derived stem cells on polyglycolic acid polylactic acid mesh |
| url | http://dx.doi.org/10.1155/2015/350718 |
| work_keys_str_mv | AT mengdeng endothelialdifferentiationofhumanadiposederivedstemcellsonpolyglycolicacidpolylacticacidmesh AT yunpenggu endothelialdifferentiationofhumanadiposederivedstemcellsonpolyglycolicacidpolylacticacidmesh AT zhenjunliu endothelialdifferentiationofhumanadiposederivedstemcellsonpolyglycolicacidpolylacticacidmesh AT yueqi endothelialdifferentiationofhumanadiposederivedstemcellsonpolyglycolicacidpolylacticacidmesh AT guiema endothelialdifferentiationofhumanadiposederivedstemcellsonpolyglycolicacidpolylacticacidmesh AT ningkang endothelialdifferentiationofhumanadiposederivedstemcellsonpolyglycolicacidpolylacticacidmesh |