Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathway
Acetaminophen (APAP)-induced acute kidney injury (APAP-AKI) has turned into one of reasons for clinic obtained renal insufficiency. Magnesium hydride (MgH2), as a solid-state hydrogen source, might be potentially applied in clinical practice. The current study aimed to investigate the protective eff...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2330629 |
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| author | Yachen Si Lulu Liu Yinyin Zhang Hongxia Li Tingting Zhao Su Liu Xuejun Sun Jin Cheng Hongtao Lu |
| author_facet | Yachen Si Lulu Liu Yinyin Zhang Hongxia Li Tingting Zhao Su Liu Xuejun Sun Jin Cheng Hongtao Lu |
| author_sort | Yachen Si |
| collection | DOAJ |
| description | Acetaminophen (APAP)-induced acute kidney injury (APAP-AKI) has turned into one of reasons for clinic obtained renal insufficiency. Magnesium hydride (MgH2), as a solid-state hydrogen source, might be potentially applied in clinical practice. The current study aimed to investigate the protective effect of MgH2 against APAP-AKI. The results showed that MgH2 improved renal function and histological injury in mice of APAP-AKI. MgH2 also had protective effects on APAP-induced cytotoxicity in HK-2 cells. In addition, the increased level of reactive oxygen species (ROS) and expressions of inflammatory cytokines (TNF-α and IL-1β) and pro-apoptotic factors (Bad, Bax, Caspase3, and CytC) induced by APAP were downregulated with MgH2 treatment. Furthermore, the expressions of molecules related to TXNIP/NLRP3/NF-κB pathway (TXNIP, NLRP3, NF-κB p65 and p-NF-κB p65) in renal tissues and HK-2 cells were enhanced by APAP overdose, which were reduced by MgH2 administration. Collectively, this study indicated that MgH2 protects against APAP-AKI by alleviating oxidative stress, inflammation and apoptosis via inhibition of TXNIP/NLRP3/NF-κB signaling pathway. |
| format | Article |
| id | doaj-art-39570c01ceea416aa8ceccd5a5714e9d |
| institution | Kabale University |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-39570c01ceea416aa8ceccd5a5714e9d2025-01-23T04:17:48ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146110.1080/0886022X.2024.2330629Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathwayYachen Si0Lulu Liu1Yinyin Zhang2Hongxia Li3Tingting Zhao4Su Liu5Xuejun Sun6Jin Cheng7Hongtao Lu8Department of Internal Medicine, No. 944 Hospital of Joint Logistics Support Force, Jiuquan, ChinaDepartment of Nephrology, Shanghai Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Naval Medicine, Naval Medical University, Shanghai, ChinaDepartment of Naval Medicine, Naval Medical University, Shanghai, ChinaDepartment of Nephrology, Shanghai Changhai Hospital, Naval Medical University, Shanghai, ChinaDepartment of Naval Medicine, Naval Medical University, Shanghai, ChinaDepartment of Naval Medicine, Naval Medical University, Shanghai, ChinaInternal Medicine III (Nephrology and Endocrinology), Naval Medical Center, Naval Medical University, Shanghai, ChinaDepartment of Naval Medicine, Naval Medical University, Shanghai, ChinaAcetaminophen (APAP)-induced acute kidney injury (APAP-AKI) has turned into one of reasons for clinic obtained renal insufficiency. Magnesium hydride (MgH2), as a solid-state hydrogen source, might be potentially applied in clinical practice. The current study aimed to investigate the protective effect of MgH2 against APAP-AKI. The results showed that MgH2 improved renal function and histological injury in mice of APAP-AKI. MgH2 also had protective effects on APAP-induced cytotoxicity in HK-2 cells. In addition, the increased level of reactive oxygen species (ROS) and expressions of inflammatory cytokines (TNF-α and IL-1β) and pro-apoptotic factors (Bad, Bax, Caspase3, and CytC) induced by APAP were downregulated with MgH2 treatment. Furthermore, the expressions of molecules related to TXNIP/NLRP3/NF-κB pathway (TXNIP, NLRP3, NF-κB p65 and p-NF-κB p65) in renal tissues and HK-2 cells were enhanced by APAP overdose, which were reduced by MgH2 administration. Collectively, this study indicated that MgH2 protects against APAP-AKI by alleviating oxidative stress, inflammation and apoptosis via inhibition of TXNIP/NLRP3/NF-κB signaling pathway.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2330629Acetaminophenacute kidney injurymagnesium hydrideoxidative stressinflammationapoptosis |
| spellingShingle | Yachen Si Lulu Liu Yinyin Zhang Hongxia Li Tingting Zhao Su Liu Xuejun Sun Jin Cheng Hongtao Lu Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathway Renal Failure Acetaminophen acute kidney injury magnesium hydride oxidative stress inflammation apoptosis |
| title | Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathway |
| title_full | Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathway |
| title_fullStr | Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathway |
| title_full_unstemmed | Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathway |
| title_short | Magnesium hydride protects against acetaminophen-induced acute kidney injury by inhibiting TXNIP/NLRP3/nf-κb pathway |
| title_sort | magnesium hydride protects against acetaminophen induced acute kidney injury by inhibiting txnip nlrp3 nf κb pathway |
| topic | Acetaminophen acute kidney injury magnesium hydride oxidative stress inflammation apoptosis |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2330629 |
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