Periostin as a Biomarker of the Amniotic Membrane
Tracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human emb...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/987185 |
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| author | Mariya P. Dobreva Larissa Lhoest Paulo N. G. Pereira Lieve Umans Susana M. Chuva de Sousa Lopes An Zwijsen |
| author_facet | Mariya P. Dobreva Larissa Lhoest Paulo N. G. Pereira Lieve Umans Susana M. Chuva de Sousa Lopes An Zwijsen |
| author_sort | Mariya P. Dobreva |
| collection | DOAJ |
| description | Tracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human embryo and the cup-shaped mouse embryo is pivotal in particular when sampling amniotic membrane from nonprimate species for isolating candidate amniotic stem cells. The availability of molecular marker genes that are specifically expressed in the amniotic membrane and not in other extraembryonic membranes would be instrumental to validate unequivocally the starting material under investigation. So far such amniotic markers have not been reported. We postulated that bone morphogenetic protein (BMP) target genes are putative amniotic membrane markers mainly because deficiency in one of several components of the BMP signaling cascade in mice has been documented to result in defective development of the early amnion. Comparative gene expression analysis of acknowledged target genes for BMP in different extraembryonic tissues, combined with in situ hybridization, identified Periostin (Postn) mRNA enrichment in amnion throughout gestation. In addition, we identify and propose a combination of markers as transcriptional signature for the different extraembryonic tissues in mouse. |
| format | Article |
| id | doaj-art-393dc05b5746448ab18524c1cc59c9dd |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-393dc05b5746448ab18524c1cc59c9dd2025-02-03T06:01:19ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/987185987185Periostin as a Biomarker of the Amniotic MembraneMariya P. Dobreva0Larissa Lhoest1Paulo N. G. Pereira2Lieve Umans3Susana M. Chuva de Sousa Lopes4An Zwijsen5Laboratory of Developmental Signaling, VIB11 Center for the Biology of Disease, VIB, 3000 Leuven, BelgiumLaboratory of Developmental Signaling, VIB11 Center for the Biology of Disease, VIB, 3000 Leuven, BelgiumLaboratory of Developmental Signaling, VIB11 Center for the Biology of Disease, VIB, 3000 Leuven, BelgiumLaboratory of Molecular Biology (Celgen), Department of Development and Regeneration, KU Leuven, 3000 Leuven, BelgiumDepartment of Anatomy and Embryology, Leiden University Medical Centre, 2333 ZC Leiden, The NetherlandsLaboratory of Developmental Signaling, VIB11 Center for the Biology of Disease, VIB, 3000 Leuven, BelgiumTracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human embryo and the cup-shaped mouse embryo is pivotal in particular when sampling amniotic membrane from nonprimate species for isolating candidate amniotic stem cells. The availability of molecular marker genes that are specifically expressed in the amniotic membrane and not in other extraembryonic membranes would be instrumental to validate unequivocally the starting material under investigation. So far such amniotic markers have not been reported. We postulated that bone morphogenetic protein (BMP) target genes are putative amniotic membrane markers mainly because deficiency in one of several components of the BMP signaling cascade in mice has been documented to result in defective development of the early amnion. Comparative gene expression analysis of acknowledged target genes for BMP in different extraembryonic tissues, combined with in situ hybridization, identified Periostin (Postn) mRNA enrichment in amnion throughout gestation. In addition, we identify and propose a combination of markers as transcriptional signature for the different extraembryonic tissues in mouse.http://dx.doi.org/10.1155/2012/987185 |
| spellingShingle | Mariya P. Dobreva Larissa Lhoest Paulo N. G. Pereira Lieve Umans Susana M. Chuva de Sousa Lopes An Zwijsen Periostin as a Biomarker of the Amniotic Membrane Stem Cells International |
| title | Periostin as a Biomarker of the Amniotic Membrane |
| title_full | Periostin as a Biomarker of the Amniotic Membrane |
| title_fullStr | Periostin as a Biomarker of the Amniotic Membrane |
| title_full_unstemmed | Periostin as a Biomarker of the Amniotic Membrane |
| title_short | Periostin as a Biomarker of the Amniotic Membrane |
| title_sort | periostin as a biomarker of the amniotic membrane |
| url | http://dx.doi.org/10.1155/2012/987185 |
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