Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i>
This study seeks to assess the impact of varying concentrations of lysophospholipids on the antioxidant capacity, digestive performance, and intestinal microbiota of <i>L. vannamei</i>. A total of 840 shrimp, with an average initial weight of 2.22 ± 0.11 g, were randomly distributed acro...
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2025-01-01
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| author | Hailiang Yan Yun Wang Hong Liang Yafei Duan Jun Wang Chuanpeng Zhou Zhong Huang |
| author_facet | Hailiang Yan Yun Wang Hong Liang Yafei Duan Jun Wang Chuanpeng Zhou Zhong Huang |
| author_sort | Hailiang Yan |
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| description | This study seeks to assess the impact of varying concentrations of lysophospholipids on the antioxidant capacity, digestive performance, and intestinal microbiota of <i>L. vannamei</i>. A total of 840 shrimp, with an average initial weight of 2.22 ± 0.11 g, were randomly distributed across 28 aquaculture tanks (30 shrimp per tank), organized into 7 distinct treatment groups, each comprising 4 replicates. The control group (DL2) was administered a basal diet formula supplemented with 2% soy lecithin, whereas the experimental groups received lysophospholipids at varying concentrations of 0% (RL0), 0.1% (RL0.1), 0.5% (RL0.5), 1% (RL1), 1.5% (RL1.5), and 2% (RL2). The results revealed that the total antioxidant capacity (T-AOC) level in the RL0.1 group was significantly elevated compared to the DL2 and RL2 groups (<i>p</i> < 0.05). Furthermore, glutathione peroxidase (GSH-Px) and catalase (CAT) activities were markedly higher in the RL1 and RL1.5 groups relative to other groups (<i>p</i> < 0.05). Superoxide dismutase (SOD) activity exhibited a significant increase across all lysophospholipid-supplemented groups when compared to the DL2 group (<i>p</i> < 0.05). Malondialdehyde (MDA) levels were notably elevated in the RL0.5, RL1.5, and RL2 groups relative to the other groups (<i>p</i> < 0.05). Regarding antioxidant-related genes in the hemocytes, the relative expression levels of <i>Nrf1</i>, <i>Nrf2</i>, <i>GPx</i>, <i>SOD</i>, <i>CAT</i>, and <i>Hippo</i> were significantly higher in the RL0.5 and RL1 groups compared to the DL2 group (<i>p</i> < 0.05). Additionally, the relative expression levels of <i>GPx</i> and <i>SOD</i> were significantly elevated in the RL2 group compared to the DL2 group (<i>p</i> < 0.05). In the intestinal tract, the activities of trypsin and α-amylase (AMS) were significantly elevated in the RL0.1, RL0.5, and RL1 groups compared to the DL2 group (<i>p</i> < 0.05). Additionally, the RL0.1 group demonstrated significantly higher lipase (LPS) activity than the other groups supplemented with lysophospholipids (<i>p</i> < 0.05). Furthermore, the relative expression levels of the <i>trypsin</i> and <i>α-amylase</i> genes were significantly increased in the RL1 and RL1.5 groups in comparison to the DL2 group (<i>p</i> < 0.05). Sequencing analysis of the intestinal microbiota indicated that the incorporation of lysophospholipids resulted in an enhancement of the composition and structure of the intestinal microbiota. The functional abundance of the intestinal microbiome was primarily enriched in metabolic pathways. Overall, the incorporation of lysophospholipids into the diet exerted a beneficial effect on the antioxidant capacity, digestive performance, and intestinal microbiota of <i>L. vannamei</i>, especially with the supplementation of 0.1% lysophospholipids. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Biology |
| spelling | doaj-art-392a506ad63d447db8bd7c1eddcd7a882025-01-24T13:23:36ZengMDPI AGBiology2079-77372025-01-011419010.3390/biology14010090Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i>Hailiang Yan0Yun Wang1Hong Liang2Yafei Duan3Jun Wang4Chuanpeng Zhou5Zhong Huang6College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaShenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, ChinaThis study seeks to assess the impact of varying concentrations of lysophospholipids on the antioxidant capacity, digestive performance, and intestinal microbiota of <i>L. vannamei</i>. A total of 840 shrimp, with an average initial weight of 2.22 ± 0.11 g, were randomly distributed across 28 aquaculture tanks (30 shrimp per tank), organized into 7 distinct treatment groups, each comprising 4 replicates. The control group (DL2) was administered a basal diet formula supplemented with 2% soy lecithin, whereas the experimental groups received lysophospholipids at varying concentrations of 0% (RL0), 0.1% (RL0.1), 0.5% (RL0.5), 1% (RL1), 1.5% (RL1.5), and 2% (RL2). The results revealed that the total antioxidant capacity (T-AOC) level in the RL0.1 group was significantly elevated compared to the DL2 and RL2 groups (<i>p</i> < 0.05). Furthermore, glutathione peroxidase (GSH-Px) and catalase (CAT) activities were markedly higher in the RL1 and RL1.5 groups relative to other groups (<i>p</i> < 0.05). Superoxide dismutase (SOD) activity exhibited a significant increase across all lysophospholipid-supplemented groups when compared to the DL2 group (<i>p</i> < 0.05). Malondialdehyde (MDA) levels were notably elevated in the RL0.5, RL1.5, and RL2 groups relative to the other groups (<i>p</i> < 0.05). Regarding antioxidant-related genes in the hemocytes, the relative expression levels of <i>Nrf1</i>, <i>Nrf2</i>, <i>GPx</i>, <i>SOD</i>, <i>CAT</i>, and <i>Hippo</i> were significantly higher in the RL0.5 and RL1 groups compared to the DL2 group (<i>p</i> < 0.05). Additionally, the relative expression levels of <i>GPx</i> and <i>SOD</i> were significantly elevated in the RL2 group compared to the DL2 group (<i>p</i> < 0.05). In the intestinal tract, the activities of trypsin and α-amylase (AMS) were significantly elevated in the RL0.1, RL0.5, and RL1 groups compared to the DL2 group (<i>p</i> < 0.05). Additionally, the RL0.1 group demonstrated significantly higher lipase (LPS) activity than the other groups supplemented with lysophospholipids (<i>p</i> < 0.05). Furthermore, the relative expression levels of the <i>trypsin</i> and <i>α-amylase</i> genes were significantly increased in the RL1 and RL1.5 groups in comparison to the DL2 group (<i>p</i> < 0.05). Sequencing analysis of the intestinal microbiota indicated that the incorporation of lysophospholipids resulted in an enhancement of the composition and structure of the intestinal microbiota. The functional abundance of the intestinal microbiome was primarily enriched in metabolic pathways. Overall, the incorporation of lysophospholipids into the diet exerted a beneficial effect on the antioxidant capacity, digestive performance, and intestinal microbiota of <i>L. vannamei</i>, especially with the supplementation of 0.1% lysophospholipids.https://www.mdpi.com/2079-7737/14/1/90<i>Litopenaeus vannamei</i>lysophospholipidsantioxidant capacitydigestionintestinal microbiota |
| spellingShingle | Hailiang Yan Yun Wang Hong Liang Yafei Duan Jun Wang Chuanpeng Zhou Zhong Huang Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i> Biology <i>Litopenaeus vannamei</i> lysophospholipids antioxidant capacity digestion intestinal microbiota |
| title | Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i> |
| title_full | Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i> |
| title_fullStr | Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i> |
| title_full_unstemmed | Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i> |
| title_short | Effects of Lysophospholipids on the Antioxidant Capacity, Digestive Performance, and Intestinal Microbiota of <i>Litopenaeus vannamei</i> |
| title_sort | effects of lysophospholipids on the antioxidant capacity digestive performance and intestinal microbiota of i litopenaeus vannamei i |
| topic | <i>Litopenaeus vannamei</i> lysophospholipids antioxidant capacity digestion intestinal microbiota |
| url | https://www.mdpi.com/2079-7737/14/1/90 |
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