Anionic liposomes prepared without organic solvents for effective siRNA delivery
Abstract Currently, organic solvents are necessary for the preparation of anionic liposomes for siRNA delivery. The removal of organic solvent is time‐consuming and the residual organic solvent is not only a hidden danger, but also affects the stability of anionic liposomes. Glycerol, which is physi...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12117 |
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| author | Xiu Han Yan Lu Zhaoluo Xu Yanan Chu Xueping Ma Haiping Wu Bingjie Zou Guohua Zhou |
| author_facet | Xiu Han Yan Lu Zhaoluo Xu Yanan Chu Xueping Ma Haiping Wu Bingjie Zou Guohua Zhou |
| author_sort | Xiu Han |
| collection | DOAJ |
| description | Abstract Currently, organic solvents are necessary for the preparation of anionic liposomes for siRNA delivery. The removal of organic solvent is time‐consuming and the residual organic solvent is not only a hidden danger, but also affects the stability of anionic liposomes. Glycerol, which is physiologically compatible and does not need to be removed, is used to promote the dispersion of lipids and the formation of anionic liposomes. Additionally, the preparation process is simple and not time‐consuming. The results showed that anionic liposomes, which were typically spherical with a particle size of 188.9 nm were successfully prepared with glycerol. And with the help of Ca2+, siRNA was encapsulated in anionic liposomes. The highest encapsulation efficiency at 2.4 mM Ca2+ reached 91%. And the formation of calcium phosphate could promote the endosomal escape of siRNA effectively. The results from cell viability showed that the anionic liposomes had no obvious cytotoxicity. It was also verified that anionic liposomes could improve the resistance of siRNA against degradation. Additionally, siRNA delivered by anionic liposomes could play an effective role in knockout. Therefore, anionic liposomes prepared with glycerol will be a safe and effective delivery platform for siRNA and even other nucleic acid drugs. |
| format | Article |
| id | doaj-art-39142f7682a04314b936be09c2447add |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-39142f7682a04314b936be09c2447add2025-02-03T01:29:43ZengWileyIET Nanobiotechnology1751-87411751-875X2023-05-0117326928010.1049/nbt2.12117Anionic liposomes prepared without organic solvents for effective siRNA deliveryXiu Han0Yan Lu1Zhaoluo Xu2Yanan Chu3Xueping Ma4Haiping Wu5Bingjie Zou6Guohua Zhou7Department of Clinical Pharmacy Affiliated Jinling Hospital Medical School of Nanjing University Nanjing ChinaDepartment of Gastroenterology Children's Hospital of Nanjing Medical University Nanjing ChinaDepartment of Clinical Pharmacy Affiliated Jinling Hospital Medical School of Nanjing University Nanjing ChinaDepartment of Clinical Pharmacy Affiliated Jinling Hospital Medical School of Nanjing University Nanjing ChinaDepartment of Clinical Pharmacy Affiliated Jinling Hospital Medical School of Nanjing University Nanjing ChinaDepartment of Clinical Pharmacy Affiliated Jinling Hospital Medical School of Nanjing University Nanjing ChinaKey Laboratory of Drug Quality Control and Pharmacovigilance Ministry of Education School of Pharmacy China Pharmaceutical University Nanjing ChinaDepartment of Clinical Pharmacy Affiliated Jinling Hospital Medical School of Nanjing University Nanjing ChinaAbstract Currently, organic solvents are necessary for the preparation of anionic liposomes for siRNA delivery. The removal of organic solvent is time‐consuming and the residual organic solvent is not only a hidden danger, but also affects the stability of anionic liposomes. Glycerol, which is physiologically compatible and does not need to be removed, is used to promote the dispersion of lipids and the formation of anionic liposomes. Additionally, the preparation process is simple and not time‐consuming. The results showed that anionic liposomes, which were typically spherical with a particle size of 188.9 nm were successfully prepared with glycerol. And with the help of Ca2+, siRNA was encapsulated in anionic liposomes. The highest encapsulation efficiency at 2.4 mM Ca2+ reached 91%. And the formation of calcium phosphate could promote the endosomal escape of siRNA effectively. The results from cell viability showed that the anionic liposomes had no obvious cytotoxicity. It was also verified that anionic liposomes could improve the resistance of siRNA against degradation. Additionally, siRNA delivered by anionic liposomes could play an effective role in knockout. Therefore, anionic liposomes prepared with glycerol will be a safe and effective delivery platform for siRNA and even other nucleic acid drugs.https://doi.org/10.1049/nbt2.12117anionic liposomeorganic solventsiRNA |
| spellingShingle | Xiu Han Yan Lu Zhaoluo Xu Yanan Chu Xueping Ma Haiping Wu Bingjie Zou Guohua Zhou Anionic liposomes prepared without organic solvents for effective siRNA delivery IET Nanobiotechnology anionic liposome organic solvent siRNA |
| title | Anionic liposomes prepared without organic solvents for effective siRNA delivery |
| title_full | Anionic liposomes prepared without organic solvents for effective siRNA delivery |
| title_fullStr | Anionic liposomes prepared without organic solvents for effective siRNA delivery |
| title_full_unstemmed | Anionic liposomes prepared without organic solvents for effective siRNA delivery |
| title_short | Anionic liposomes prepared without organic solvents for effective siRNA delivery |
| title_sort | anionic liposomes prepared without organic solvents for effective sirna delivery |
| topic | anionic liposome organic solvent siRNA |
| url | https://doi.org/10.1049/nbt2.12117 |
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