Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells
<b>Background:</b> Energy delivered at different wavelengths causes different types of damage to DNA. <b>Methods:</b> PC-3, FaDu, 4T1 and B16-F10 cells were irradiated with different wavelengths of ultraviolet light (UVA/UVC) and ionizing radiation (X-ray). Furthermore, diffe...
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MDPI AG
2024-12-01
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| author | Katja Tietze Florian Brandt Kerstin Wetzig Lisa Hübinger Marc Pretze Ralph Alexander Bundschuh Jörg Kotzerke |
| author_facet | Katja Tietze Florian Brandt Kerstin Wetzig Lisa Hübinger Marc Pretze Ralph Alexander Bundschuh Jörg Kotzerke |
| author_sort | Katja Tietze |
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| description | <b>Background:</b> Energy delivered at different wavelengths causes different types of damage to DNA. <b>Methods:</b> PC-3, FaDu, 4T1 and B16-F10 cells were irradiated with different wavelengths of ultraviolet light (UVA/UVC) and ionizing radiation (X-ray). Furthermore, different photosensitizers (ortho-iodo-Hoechst33258/psoralen/trioxsalen) were tested for their amplifying effect. Survival fraction and damage analysis using the <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="normal">γ</mi></semantics></math></inline-formula>H2A.X assay (double-strand breaks) and the ELISA assay (cyclobutane pyrimidine dimers) were compared. <b>Results:</b> The PC-3 cells were found to be the most sensitive cells to the treatment strategies used. FaDu and PC-3 showed a strong sensitivity to UVA. Analysis of the damage showed that the cell lines exhibited different sensitivities. <b>Conclusions:</b> Thus, an enhancing effect of photosensitizers (PS) in combination with UVA could be demonstrated in some cases. However, this is cell- and dose-dependent. |
| format | Article |
| id | doaj-art-38c2c01d93d24d39a00a7ea829709b64 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-38c2c01d93d24d39a00a7ea829709b642025-01-24T13:23:55ZengMDPI AGBiomedicines2227-90592024-12-011317310.3390/biomedicines13010073Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 CellsKatja Tietze0Florian Brandt1Kerstin Wetzig2Lisa Hübinger3Marc Pretze4Ralph Alexander Bundschuh5Jörg Kotzerke6Department of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany<b>Background:</b> Energy delivered at different wavelengths causes different types of damage to DNA. <b>Methods:</b> PC-3, FaDu, 4T1 and B16-F10 cells were irradiated with different wavelengths of ultraviolet light (UVA/UVC) and ionizing radiation (X-ray). Furthermore, different photosensitizers (ortho-iodo-Hoechst33258/psoralen/trioxsalen) were tested for their amplifying effect. Survival fraction and damage analysis using the <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="normal">γ</mi></semantics></math></inline-formula>H2A.X assay (double-strand breaks) and the ELISA assay (cyclobutane pyrimidine dimers) were compared. <b>Results:</b> The PC-3 cells were found to be the most sensitive cells to the treatment strategies used. FaDu and PC-3 showed a strong sensitivity to UVA. Analysis of the damage showed that the cell lines exhibited different sensitivities. <b>Conclusions:</b> Thus, an enhancing effect of photosensitizers (PS) in combination with UVA could be demonstrated in some cases. However, this is cell- and dose-dependent.https://www.mdpi.com/2227-9059/13/1/73X-raysUVA/UVCphotosensitizerortho-iodo-hoechst33258psoralentrioxsalen |
| spellingShingle | Katja Tietze Florian Brandt Kerstin Wetzig Lisa Hübinger Marc Pretze Ralph Alexander Bundschuh Jörg Kotzerke Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells Biomedicines X-rays UVA/UVC photosensitizer ortho-iodo-hoechst33258 psoralen trioxsalen |
| title | Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells |
| title_full | Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells |
| title_fullStr | Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells |
| title_full_unstemmed | Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells |
| title_short | Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells |
| title_sort | comparison of radio and phototoxicity in association with an enhancing effect of the photosensitizers psoralen trioxsalen and ortho iodo hoechst33258 on fadu pc 3 4t1 and b16 f10 cells |
| topic | X-rays UVA/UVC photosensitizer ortho-iodo-hoechst33258 psoralen trioxsalen |
| url | https://www.mdpi.com/2227-9059/13/1/73 |
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