Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitro
The increasing exposure to biomass-burning emissions underscores the need to understand their toxicological impacts on human health. In this study, we developed a laboratory model to evaluate the effects of single and repeated sub-acute exposures to water-soluble wood tar (WT) extracts, a product of...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015677 |
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| author | Michal Pardo Chunlin Li Amani Jabali Yinon Rudich |
| author_facet | Michal Pardo Chunlin Li Amani Jabali Yinon Rudich |
| author_sort | Michal Pardo |
| collection | DOAJ |
| description | The increasing exposure to biomass-burning emissions underscores the need to understand their toxicological impacts on human health. In this study, we developed a laboratory model to evaluate the effects of single and repeated sub-acute exposures to water-soluble wood tar (WT) extracts, a product of biomass burning, on human lung, liver, and immune cells. Using representative cell lines for different tissues, we examined the cytotoxic effects under conditions mimicking sub-acute environmental exposure levels relevant to humans. Our findings indicate that repeated sub-acute exposures to water-soluble WT extracts significantly enhance the inflammatory response, evidenced by increased IL6, IL8, and TNFa cytokine levels, compared to a single exposure. Additionally, oxidative stress responses were more pronounced with increased lipid peroxidation and HMOX1, GCLC and CYP1A1 gene expression following repeated exposures. Metabolomics analyses of polar and lipid metabolites revealed changes related to energy production and consumption that emerge even after a single exposure at sub-acute levels and vary across different cell types representing the different tissues. Impaired cellular respiration, measured by oxygen consumption rate, corroborates the observed changes. These results provide important insights into the cellular mechanisms driving the response to biomass-burning exposure and highlight the potential health risks associated with sub-acute exposure to environmental pollutants. |
| format | Article |
| id | doaj-art-38c1754753ec4d089a546fa22d171676 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-38c1754753ec4d089a546fa22d1716762025-01-23T05:25:45ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117491Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitroMichal Pardo0Chunlin Li1Amani Jabali2Yinon Rudich3Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, Rehovot 7610001, Israel; Corresponding author.College of Environmental Science and Engineering, Tongji University, Shanghai 200072, ChinaDepartment of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, Rehovot 7610001, IsraelDepartment of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, Rehovot 7610001, IsraelThe increasing exposure to biomass-burning emissions underscores the need to understand their toxicological impacts on human health. In this study, we developed a laboratory model to evaluate the effects of single and repeated sub-acute exposures to water-soluble wood tar (WT) extracts, a product of biomass burning, on human lung, liver, and immune cells. Using representative cell lines for different tissues, we examined the cytotoxic effects under conditions mimicking sub-acute environmental exposure levels relevant to humans. Our findings indicate that repeated sub-acute exposures to water-soluble WT extracts significantly enhance the inflammatory response, evidenced by increased IL6, IL8, and TNFa cytokine levels, compared to a single exposure. Additionally, oxidative stress responses were more pronounced with increased lipid peroxidation and HMOX1, GCLC and CYP1A1 gene expression following repeated exposures. Metabolomics analyses of polar and lipid metabolites revealed changes related to energy production and consumption that emerge even after a single exposure at sub-acute levels and vary across different cell types representing the different tissues. Impaired cellular respiration, measured by oxygen consumption rate, corroborates the observed changes. These results provide important insights into the cellular mechanisms driving the response to biomass-burning exposure and highlight the potential health risks associated with sub-acute exposure to environmental pollutants.http://www.sciencedirect.com/science/article/pii/S0147651324015677Biomass burningMetabolic profilingSub-acute exposureSystemic effectExposure model |
| spellingShingle | Michal Pardo Chunlin Li Amani Jabali Yinon Rudich Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitro Ecotoxicology and Environmental Safety Biomass burning Metabolic profiling Sub-acute exposure Systemic effect Exposure model |
| title | Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitro |
| title_full | Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitro |
| title_fullStr | Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitro |
| title_full_unstemmed | Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitro |
| title_short | Cellular and metabolic impacts of repeated sub-acute exposures to biomass-burning extracts in vitro |
| title_sort | cellular and metabolic impacts of repeated sub acute exposures to biomass burning extracts in vitro |
| topic | Biomass burning Metabolic profiling Sub-acute exposure Systemic effect Exposure model |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015677 |
| work_keys_str_mv | AT michalpardo cellularandmetabolicimpactsofrepeatedsubacuteexposurestobiomassburningextractsinvitro AT chunlinli cellularandmetabolicimpactsofrepeatedsubacuteexposurestobiomassburningextractsinvitro AT amanijabali cellularandmetabolicimpactsofrepeatedsubacuteexposurestobiomassburningextractsinvitro AT yinonrudich cellularandmetabolicimpactsofrepeatedsubacuteexposurestobiomassburningextractsinvitro |