Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan
The clinical outcomes of patients infected with hepatitis C virus (HCV) range from acute resolving hepatitis to chronic liver diseases such as liver cirrhosis or hepatocellular carcinoma. Identification of the infecting virus genotype is indispensable for the exploration of many aspects of HCV infec...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2014/606201 |
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| author | Sajid Ali Ayaz Ahmad Raham Sher Khan Sanaullah Khan Muhammad Hamayun Sumera Afzal Khan Amjad Iqbal Abid Ali Khan Abdul Wadood Taj Ur Rahman Ali Hydar Baig |
| author_facet | Sajid Ali Ayaz Ahmad Raham Sher Khan Sanaullah Khan Muhammad Hamayun Sumera Afzal Khan Amjad Iqbal Abid Ali Khan Abdul Wadood Taj Ur Rahman Ali Hydar Baig |
| author_sort | Sajid Ali |
| collection | DOAJ |
| description | The clinical outcomes of patients infected with hepatitis C virus (HCV) range from acute resolving hepatitis to chronic liver diseases such as liver cirrhosis or hepatocellular carcinoma. Identification of the infecting virus genotype is indispensable for the exploration of many aspects of HCV infection, including epidemiology, pathogenesis, and response to antiviral therapy. 1419 individuals were screened for anti-HCV in this study, of which 166 (11.7%) were found reactive by ICT (Immunochromatographic test). These 166 anti-HCV positive and 26 normal individuals were further analyzed. RNA was extracted from serum and reverse-transcribed to cDNA and the core region of HCV genome was targeted and amplified by multiplex PCR. HCV RNA was detected in 121 individuals, of which 87 were male and 34 were female. Genotype 3a was the most prevalent among all the genotypes observed followed by 3b. Genotypes 1a, 2a, and 2b were found in 10.89%, 13.22%, and 6.61% patients, respectively. 25.41% of the HCV RNA positive samples were not typed. 6.05% of patients were found having mixed genotypes. These findings will not only help the physicians to prescribe more appropriate treatment for the HCV infection but will also draw the attention of health-related policy makers to devise strategies to curb the disease more effectively. |
| format | Article |
| id | doaj-art-38aadba956f6474ab34ce259013ef8f5 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-38aadba956f6474ab34ce259013ef8f52025-02-03T01:31:19ZengWileyAdvances in Virology1687-86391687-86472014-01-01201410.1155/2014/606201606201Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, PakistanSajid Ali0Ayaz Ahmad1Raham Sher Khan2Sanaullah Khan3Muhammad Hamayun4Sumera Afzal Khan5Amjad Iqbal6Abid Ali Khan7Abdul Wadood8Taj Ur Rahman9Ali Hydar Baig10Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, PakistanDepartment of Biotechnology, KUST, Kohat 26000, PakistanDepartment of Botany, Abdul Wali Khan University Mardan, Mardan 23200, PakistanCenter of Biotechnology & Microbiology, University of Peshawar, Peshawar 25120, PakistanDepartment of Agriculture, Abdul Wali Khan University Mardan, Mardan 23200, PakistanCenter of Biotechnology & Microbiology, University of Peshawar, Peshawar 25120, PakistanDepartment of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, PakistanDepartment of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, PakistanDepartment of Biotechnology, KUST, Kohat 26000, PakistanThe clinical outcomes of patients infected with hepatitis C virus (HCV) range from acute resolving hepatitis to chronic liver diseases such as liver cirrhosis or hepatocellular carcinoma. Identification of the infecting virus genotype is indispensable for the exploration of many aspects of HCV infection, including epidemiology, pathogenesis, and response to antiviral therapy. 1419 individuals were screened for anti-HCV in this study, of which 166 (11.7%) were found reactive by ICT (Immunochromatographic test). These 166 anti-HCV positive and 26 normal individuals were further analyzed. RNA was extracted from serum and reverse-transcribed to cDNA and the core region of HCV genome was targeted and amplified by multiplex PCR. HCV RNA was detected in 121 individuals, of which 87 were male and 34 were female. Genotype 3a was the most prevalent among all the genotypes observed followed by 3b. Genotypes 1a, 2a, and 2b were found in 10.89%, 13.22%, and 6.61% patients, respectively. 25.41% of the HCV RNA positive samples were not typed. 6.05% of patients were found having mixed genotypes. These findings will not only help the physicians to prescribe more appropriate treatment for the HCV infection but will also draw the attention of health-related policy makers to devise strategies to curb the disease more effectively.http://dx.doi.org/10.1155/2014/606201 |
| spellingShingle | Sajid Ali Ayaz Ahmad Raham Sher Khan Sanaullah Khan Muhammad Hamayun Sumera Afzal Khan Amjad Iqbal Abid Ali Khan Abdul Wadood Taj Ur Rahman Ali Hydar Baig Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan Advances in Virology |
| title | Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan |
| title_full | Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan |
| title_fullStr | Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan |
| title_full_unstemmed | Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan |
| title_short | Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan |
| title_sort | genotyping of hcv rna reveals that 3a is the most prevalent genotype in mardan pakistan |
| url | http://dx.doi.org/10.1155/2014/606201 |
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