Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae
The intraspecies and interspecies Comparative Genomic Hybridization (CGH) between the closely related Cebidae species, capuchin monkeys (<i>Cebus capucinus</i>, <i>Sapajus apella</i>), and the tamarins (<i>Saguinus mystax, Leontocebus fuscicollis</i>) was performe...
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| author | Vanessa Milioto Vincenzo Arizza Aiti Vizzini Polina L. Perelman Melody E. Roelke-Parker Francesca Dumas |
| author_facet | Vanessa Milioto Vincenzo Arizza Aiti Vizzini Polina L. Perelman Melody E. Roelke-Parker Francesca Dumas |
| author_sort | Vanessa Milioto |
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| description | The intraspecies and interspecies Comparative Genomic Hybridization (CGH) between the closely related Cebidae species, capuchin monkeys (<i>Cebus capucinus</i>, <i>Sapajus apella</i>), and the tamarins (<i>Saguinus mystax, Leontocebus fuscicollis</i>) was performed to analyze their genomes. In particular, this approach determines balanced and unbalanced repetitive DNA sequence distribution and reveals dynamics during evolution. Capuchin monkeys are considered the most ancestral group with conserved syntenies compared to the hypothetical ancestral New World monkeys’ karyotype. Also, more derived karyotypes of phylogenetically distant species from the <i>Saguinus</i> and <i>Leontocebus</i> genera are analyzed here. The distribution of repetitive sequences has been traditionally studied through classical staining methods of cytogenetics. It has been hypothesized that repeats are species-specific and their conservation across closely related species are also common; their role in the genome has been extensively studied even though its role in speciation is not well studied and understood. The CGH shows bright signals with balanced and imbalanced DNA involving different genome regions: such as predominantly repetitive DNA at centromeric positions, and interstitial distribution with extended blocks. Cross-species CGH demonstrated the origin of some heterochromatic regions and identified apomorphic heterochromatin expansion events. The uncovered distribution of repetitive sequences is analyzed from an evolutionary perspective to elucidate the genomic dynamics of the repetitive sequences at the level of chromosomal organization. |
| format | Article |
| id | doaj-art-386a4da3792f470fbb9400235c011515 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-386a4da3792f470fbb9400235c0115152025-01-24T13:23:20ZengMDPI AGBiology2079-77372024-12-011412210.3390/biology14010022Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and CallitrichinaeVanessa Milioto0Vincenzo Arizza1Aiti Vizzini2Polina L. Perelman3Melody E. Roelke-Parker4Francesca Dumas5Department of “Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF)”, University of Palermo, 90123 Palermo, ItalyDepartment of “Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF)”, University of Palermo, 90123 Palermo, ItalyDepartment of “Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF)”, University of Palermo, 90123 Palermo, ItalyInstitute of Molecular and Cellular Biology, SB RAS, Novosibirsk 630090, RussiaLaboratory Animal Sciences Program, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USADepartment of “Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF)”, University of Palermo, 90123 Palermo, ItalyThe intraspecies and interspecies Comparative Genomic Hybridization (CGH) between the closely related Cebidae species, capuchin monkeys (<i>Cebus capucinus</i>, <i>Sapajus apella</i>), and the tamarins (<i>Saguinus mystax, Leontocebus fuscicollis</i>) was performed to analyze their genomes. In particular, this approach determines balanced and unbalanced repetitive DNA sequence distribution and reveals dynamics during evolution. Capuchin monkeys are considered the most ancestral group with conserved syntenies compared to the hypothetical ancestral New World monkeys’ karyotype. Also, more derived karyotypes of phylogenetically distant species from the <i>Saguinus</i> and <i>Leontocebus</i> genera are analyzed here. The distribution of repetitive sequences has been traditionally studied through classical staining methods of cytogenetics. It has been hypothesized that repeats are species-specific and their conservation across closely related species are also common; their role in the genome has been extensively studied even though its role in speciation is not well studied and understood. The CGH shows bright signals with balanced and imbalanced DNA involving different genome regions: such as predominantly repetitive DNA at centromeric positions, and interstitial distribution with extended blocks. Cross-species CGH demonstrated the origin of some heterochromatic regions and identified apomorphic heterochromatin expansion events. The uncovered distribution of repetitive sequences is analyzed from an evolutionary perspective to elucidate the genomic dynamics of the repetitive sequences at the level of chromosomal organization.https://www.mdpi.com/2079-7737/14/1/22heterochromatinpatterndynamicevolution |
| spellingShingle | Vanessa Milioto Vincenzo Arizza Aiti Vizzini Polina L. Perelman Melody E. Roelke-Parker Francesca Dumas Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae Biology heterochromatin pattern dynamic evolution |
| title | Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae |
| title_full | Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae |
| title_fullStr | Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae |
| title_full_unstemmed | Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae |
| title_short | Comparative Genomic Hybridization (CGH) in New World Monkeys (Primates) Reveals the Distribution of Repetitive Sequences in Cebinae and Callitrichinae |
| title_sort | comparative genomic hybridization cgh in new world monkeys primates reveals the distribution of repetitive sequences in cebinae and callitrichinae |
| topic | heterochromatin pattern dynamic evolution |
| url | https://www.mdpi.com/2079-7737/14/1/22 |
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