Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice
The dextran sodium sulphate (DSS) induced colitis in mice was used as a experimental model to study the contractility of murine longitudinal colonic smooth muscle during inflammation. Smooth muscle segments of proximal, middle and distal colon were mounted in organ baths . Smooth muscle contraction...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359890974 |
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| author | J. D. van Bergeijk H. van Westreenen P. Adhien F. J. Zijlstra |
| author_facet | J. D. van Bergeijk H. van Westreenen P. Adhien F. J. Zijlstra |
| author_sort | J. D. van Bergeijk |
| collection | DOAJ |
| description | The dextran sodium sulphate (DSS) induced colitis in mice was used as a experimental model to study the contractility of murine longitudinal colonic smooth muscle during inflammation. Smooth muscle segments
of proximal, middle and distal colon were mounted in organ baths . Smooth muscle contraction was induced by carbachol, showing an aboral increase in activity, where as in the inflamed middle colonics egment a marked decr ease in activity was observed. The dilatative effect of sodium-nitroprus -side (SNP) as a nitric oxide donor was investigated after precontraction by carbachol. Both in normal and DSS segments administration of SNP to isolated
mouse colonic smooth muscle preparations caused regional differences in relaxation, the highest relaxation seen in normal proximal colonic tissue. However, this relaxation was markedly reduced in inflamed proximal preparations , associated with a diminished cGMP contents . |
| format | Article |
| id | doaj-art-382ad06cd3e8435d9a2c369835b1a445 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-382ad06cd3e8435d9a2c369835b1a4452025-02-03T01:04:48ZengWileyMediators of Inflammation0962-93511466-18611998-01-017428328710.1080/09629359890974Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in miceJ. D. van Bergeijk0H. van Westreenen1P. Adhien2F. J. Zijlstra3Department of Gastroenterology, University Hospital Dijkzigt, Dr Molewaterplein 40, Rotterdam 3015 GD, The NetherlandsDepartment of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsThe dextran sodium sulphate (DSS) induced colitis in mice was used as a experimental model to study the contractility of murine longitudinal colonic smooth muscle during inflammation. Smooth muscle segments of proximal, middle and distal colon were mounted in organ baths . Smooth muscle contraction was induced by carbachol, showing an aboral increase in activity, where as in the inflamed middle colonics egment a marked decr ease in activity was observed. The dilatative effect of sodium-nitroprus -side (SNP) as a nitric oxide donor was investigated after precontraction by carbachol. Both in normal and DSS segments administration of SNP to isolated mouse colonic smooth muscle preparations caused regional differences in relaxation, the highest relaxation seen in normal proximal colonic tissue. However, this relaxation was markedly reduced in inflamed proximal preparations , associated with a diminished cGMP contents .http://dx.doi.org/10.1080/09629359890974ColitisMiceContractilityCarbacholNitric oxidecGMP. |
| spellingShingle | J. D. van Bergeijk H. van Westreenen P. Adhien F. J. Zijlstra Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice Mediators of Inflammation Colitis Mice Contractility Carbachol Nitric oxide cGMP. |
| title | Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice |
| title_full | Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice |
| title_fullStr | Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice |
| title_full_unstemmed | Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice |
| title_short | Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice |
| title_sort | diminished nitroprusside induced relaxation of inflamed colonic smooth muscle in mice |
| topic | Colitis Mice Contractility Carbachol Nitric oxide cGMP. |
| url | http://dx.doi.org/10.1080/09629359890974 |
| work_keys_str_mv | AT jdvanbergeijk diminishednitroprussideinducedrelaxationofinflamedcolonicsmoothmuscleinmice AT hvanwestreenen diminishednitroprussideinducedrelaxationofinflamedcolonicsmoothmuscleinmice AT padhien diminishednitroprussideinducedrelaxationofinflamedcolonicsmoothmuscleinmice AT fjzijlstra diminishednitroprussideinducedrelaxationofinflamedcolonicsmoothmuscleinmice |