Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts
Nutritional intake is closely linked to gonadal development, although the mechanisms by which food intake affects gonadal development are not fully understood. Cholecystokinin (CCK) is a satiety neuropeptide derived from the hypothalamus, and the present study observed that hypothalamic CCK expressi...
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2025-01-01
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| author | Hangyu Li Hongwei Liang Xiaowen Gao Xiangtong Zeng Shuo Zheng Linlin Wang Faming Yuan Shaohua Xu Zhan Yin Guangfu Hu |
| author_facet | Hangyu Li Hongwei Liang Xiaowen Gao Xiangtong Zeng Shuo Zheng Linlin Wang Faming Yuan Shaohua Xu Zhan Yin Guangfu Hu |
| author_sort | Hangyu Li |
| collection | DOAJ |
| description | Nutritional intake is closely linked to gonadal development, although the mechanisms by which food intake affects gonadal development are not fully understood. Cholecystokinin (CCK) is a satiety neuropeptide derived from the hypothalamus, and the present study observed that hypothalamic CCK expression is significantly influenced by food intake, which is mediated through blood glucose levels. Interestingly, CCK and its receptors were observed to exhibit a high expression in the hypothalamus–pituitary–gonad (HPG) axis of grass carp (<i>Ctenopharyngodon idellus</i>), suggesting that CCK is potentially involved in regulating fish reproduction through the HPG axis. Further investigations revealed that CCK could significantly stimulate the expression of gonadotropin-releasing hormone-3 (GnRH3) in the hypothalamus. In addition, single-cell RNA sequencing showed that <i>cckrb</i> was highly enriched in pituitary follicle-stimulating hormone (FSH) cells. Further study confirmed that CCK can significantly induce FSH synthesis and secretion in primary cultured pituitary cells. Additionally, with primary cultured ovary cells as a model, the in vitro experiment demonstrated that CCK directly induces the expression of <i>lhr</i>, <i>fshr</i>, and <i>cyp19a1a</i> mRNA. This indicates that hypothalamic CCK may act as a nutrient sensor involved in regulating gonadal development in teleosts. |
| format | Article |
| id | doaj-art-381278adf5294bb49fa40439d63472f8 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-381278adf5294bb49fa40439d63472f82025-01-24T13:26:35ZengMDPI AGCells2073-44092025-01-011427810.3390/cells14020078Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in TeleostsHangyu Li0Hongwei Liang1Xiaowen Gao2Xiangtong Zeng3Shuo Zheng4Linlin Wang5Faming Yuan6Shaohua Xu7Zhan Yin8Guangfu Hu9Hubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Hongshan Laboratory, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaNutritional intake is closely linked to gonadal development, although the mechanisms by which food intake affects gonadal development are not fully understood. Cholecystokinin (CCK) is a satiety neuropeptide derived from the hypothalamus, and the present study observed that hypothalamic CCK expression is significantly influenced by food intake, which is mediated through blood glucose levels. Interestingly, CCK and its receptors were observed to exhibit a high expression in the hypothalamus–pituitary–gonad (HPG) axis of grass carp (<i>Ctenopharyngodon idellus</i>), suggesting that CCK is potentially involved in regulating fish reproduction through the HPG axis. Further investigations revealed that CCK could significantly stimulate the expression of gonadotropin-releasing hormone-3 (GnRH3) in the hypothalamus. In addition, single-cell RNA sequencing showed that <i>cckrb</i> was highly enriched in pituitary follicle-stimulating hormone (FSH) cells. Further study confirmed that CCK can significantly induce FSH synthesis and secretion in primary cultured pituitary cells. Additionally, with primary cultured ovary cells as a model, the in vitro experiment demonstrated that CCK directly induces the expression of <i>lhr</i>, <i>fshr</i>, and <i>cyp19a1a</i> mRNA. This indicates that hypothalamic CCK may act as a nutrient sensor involved in regulating gonadal development in teleosts.https://www.mdpi.com/2073-4409/14/2/78satiety neuropeptidefollicle-stimulating hormoneGnRH3pituitarysexual maturity |
| spellingShingle | Hangyu Li Hongwei Liang Xiaowen Gao Xiangtong Zeng Shuo Zheng Linlin Wang Faming Yuan Shaohua Xu Zhan Yin Guangfu Hu Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts Cells satiety neuropeptide follicle-stimulating hormone GnRH3 pituitary sexual maturity |
| title | Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts |
| title_full | Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts |
| title_fullStr | Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts |
| title_full_unstemmed | Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts |
| title_short | Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts |
| title_sort | cholecystokinin cck is a mediator between nutritional intake and gonadal development in teleosts |
| topic | satiety neuropeptide follicle-stimulating hormone GnRH3 pituitary sexual maturity |
| url | https://www.mdpi.com/2073-4409/14/2/78 |
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