Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation
Two types of culture systems were used (continuous and batch) which were fed using a simulated absorbent from a scrubber with carbonate/bicarbonate as the carbon source and nitrate as the nitrogen source by a thermophile strain, Thermosynechococcus sp. CL-1 (TCL-1) at 50°C. The lipid, carbohydrate,...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/5247265 |
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| author | Tseng Chi-Ming Ko Tzu-Hsing Hsueh Hsin-Ta Chen Hsing-Hui Ray Dah-Tong Shen Yun-Hwei Chu Hsin |
| author_facet | Tseng Chi-Ming Ko Tzu-Hsing Hsueh Hsin-Ta Chen Hsing-Hui Ray Dah-Tong Shen Yun-Hwei Chu Hsin |
| author_sort | Tseng Chi-Ming |
| collection | DOAJ |
| description | Two types of culture systems were used (continuous and batch) which were fed using a simulated absorbent from a scrubber with carbonate/bicarbonate as the carbon source and nitrate as the nitrogen source by a thermophile strain, Thermosynechococcus sp. CL-1 (TCL-1) at 50°C. The lipid, carbohydrate, and protein cellular components which can be used as bioenergy precursors along with their content as a function of various C/N ratios are quantified. Maximum lipid productivity of about 150 mg L−1 d−1 is obtained while the CO2 uptake rate is 917 mg L−1 d−1 at a dilution rate of 0.06 h−1 when both carbon and nitrogen sources are not limited. With high range of nitrogen concentrations batch culture test, TCL-1 reveals extra-high affinity on nitrogen source under limited carbon source conditions since the affinity constant is 0.12 mM. In addition, the flow of carbon fixed during photosynthesis seems to switch from the protein synthesis pathway to forming carbohydrate rather than lipid under N-limitation and a high C/N ratio for TCL-1, resulting in a maximal carbohydrate content of 61%. Consequently, TCL-1 is an appropriate candidate to treat the wastewater of environment and produce the bioenergy precursors under extreme limited nitrogen conditions. |
| format | Article |
| id | doaj-art-37ec79a43aed45edbccb5caf36515706 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-37ec79a43aed45edbccb5caf365157062025-02-03T01:32:58ZengWileyJournal of Chemistry2090-90632090-90712016-01-01201610.1155/2016/52472655247265Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon BiofixationTseng Chi-Ming0Ko Tzu-Hsing1Hsueh Hsin-Ta2Chen Hsing-Hui3Ray Dah-Tong4Shen Yun-Hwei5Chu Hsin6Department of Resources Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Tea Science, Anxi College of Tea Science, Fujian Agricultural and Forestry University, Fuzhou 350002, ChinaSustainable Environment Research Laboratories, National Cheng Kung University, Tainan, TaiwanDepartment of Environmental Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Resources Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Resources Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Environmental Engineering, National Cheng Kung University, Tainan, TaiwanTwo types of culture systems were used (continuous and batch) which were fed using a simulated absorbent from a scrubber with carbonate/bicarbonate as the carbon source and nitrate as the nitrogen source by a thermophile strain, Thermosynechococcus sp. CL-1 (TCL-1) at 50°C. The lipid, carbohydrate, and protein cellular components which can be used as bioenergy precursors along with their content as a function of various C/N ratios are quantified. Maximum lipid productivity of about 150 mg L−1 d−1 is obtained while the CO2 uptake rate is 917 mg L−1 d−1 at a dilution rate of 0.06 h−1 when both carbon and nitrogen sources are not limited. With high range of nitrogen concentrations batch culture test, TCL-1 reveals extra-high affinity on nitrogen source under limited carbon source conditions since the affinity constant is 0.12 mM. In addition, the flow of carbon fixed during photosynthesis seems to switch from the protein synthesis pathway to forming carbohydrate rather than lipid under N-limitation and a high C/N ratio for TCL-1, resulting in a maximal carbohydrate content of 61%. Consequently, TCL-1 is an appropriate candidate to treat the wastewater of environment and produce the bioenergy precursors under extreme limited nitrogen conditions.http://dx.doi.org/10.1155/2016/5247265 |
| spellingShingle | Tseng Chi-Ming Ko Tzu-Hsing Hsueh Hsin-Ta Chen Hsing-Hui Ray Dah-Tong Shen Yun-Hwei Chu Hsin Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation Journal of Chemistry |
| title | Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation |
| title_full | Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation |
| title_fullStr | Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation |
| title_full_unstemmed | Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation |
| title_short | Cellular Composition Changes and Nitrogen Uptake under Extra-Limited Nitrogen Conditions by Thermosynechococcus sp. CL-1 Carbon Biofixation |
| title_sort | cellular composition changes and nitrogen uptake under extra limited nitrogen conditions by thermosynechococcus sp cl 1 carbon biofixation |
| url | http://dx.doi.org/10.1155/2016/5247265 |
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