Personal glucose meter-based portable method for creatine kinase assay
We herein developed a simple and label-free method to detect the creatine kinase (CK) activity by employing a personal glucose meter (PGM) as a detection device. This method relies on the target-induced consumption of glucose by the cascade enzymatic reaction (CER) promoted by the combined activitie...
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Elsevier
2025-03-01
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| Series: | Biosensors and Bioelectronics: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137025000020 |
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| author | Jinhwan Lee Yejin Oh Hyoyong Kim Sangmo Lee Hyun Gyu Park |
| author_facet | Jinhwan Lee Yejin Oh Hyoyong Kim Sangmo Lee Hyun Gyu Park |
| author_sort | Jinhwan Lee |
| collection | DOAJ |
| description | We herein developed a simple and label-free method to detect the creatine kinase (CK) activity by employing a personal glucose meter (PGM) as a detection device. This method relies on the target-induced consumption of glucose by the cascade enzymatic reaction (CER) promoted by the combined activities of CK and hexokinase (HK). The target CK first catalyzes the phosphorylation of adenosine 5′-diphosphate (ADP) to adenosine 5′-triphosphate (ATP) using creatine phosphate (CP) as a phosphate donor. HK then phosphorylates glucose into glucose-6-phosphate by converting the produced ATP back to ADP, which could enter another cycle of the CER to further phosphorylate glucose in a sample. As a consequence, the glucose level would decrease depending on the CK activity, which could be very conveniently detected using a PGM. With this unique design principle, we successfully determined the CK activity down to 0.0147 U/mL with high specificity against various non-target enzymes. We also verified that the developed strategy can reliably detect CK activity in heterogeneous human blood, ensuring its robust utility in various clinical applications. This user-friendly method enables point-of care detection of CK, offering a practical tool for monitoring muscle health. By allowing simultaneous tracking of CK and glucose levels with the same PGM device, this approach is particularly beneficial for diabetes mellitus (DM) patients, empowering them to proactively manage their health and reduce the risk of muscle-related complications. |
| format | Article |
| id | doaj-art-37d1a942d4a74b4f84c91f2ad4844786 |
| institution | Kabale University |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-37d1a942d4a74b4f84c91f2ad48447862025-01-22T05:43:56ZengElsevierBiosensors and Bioelectronics: X2590-13702025-03-0122100575Personal glucose meter-based portable method for creatine kinase assayJinhwan Lee0Yejin Oh1Hyoyong Kim2Sangmo Lee3Hyun Gyu Park4Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of KoreaDepartment of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of KoreaDepartment of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of KoreaDepartment of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of KoreaCorresponding author.; Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of KoreaWe herein developed a simple and label-free method to detect the creatine kinase (CK) activity by employing a personal glucose meter (PGM) as a detection device. This method relies on the target-induced consumption of glucose by the cascade enzymatic reaction (CER) promoted by the combined activities of CK and hexokinase (HK). The target CK first catalyzes the phosphorylation of adenosine 5′-diphosphate (ADP) to adenosine 5′-triphosphate (ATP) using creatine phosphate (CP) as a phosphate donor. HK then phosphorylates glucose into glucose-6-phosphate by converting the produced ATP back to ADP, which could enter another cycle of the CER to further phosphorylate glucose in a sample. As a consequence, the glucose level would decrease depending on the CK activity, which could be very conveniently detected using a PGM. With this unique design principle, we successfully determined the CK activity down to 0.0147 U/mL with high specificity against various non-target enzymes. We also verified that the developed strategy can reliably detect CK activity in heterogeneous human blood, ensuring its robust utility in various clinical applications. This user-friendly method enables point-of care detection of CK, offering a practical tool for monitoring muscle health. By allowing simultaneous tracking of CK and glucose levels with the same PGM device, this approach is particularly beneficial for diabetes mellitus (DM) patients, empowering them to proactively manage their health and reduce the risk of muscle-related complications.http://www.sciencedirect.com/science/article/pii/S2590137025000020Personal glucose meterCreatine kinaseHexokinaseEnzyme assayStatin-associated muscle symptoms |
| spellingShingle | Jinhwan Lee Yejin Oh Hyoyong Kim Sangmo Lee Hyun Gyu Park Personal glucose meter-based portable method for creatine kinase assay Biosensors and Bioelectronics: X Personal glucose meter Creatine kinase Hexokinase Enzyme assay Statin-associated muscle symptoms |
| title | Personal glucose meter-based portable method for creatine kinase assay |
| title_full | Personal glucose meter-based portable method for creatine kinase assay |
| title_fullStr | Personal glucose meter-based portable method for creatine kinase assay |
| title_full_unstemmed | Personal glucose meter-based portable method for creatine kinase assay |
| title_short | Personal glucose meter-based portable method for creatine kinase assay |
| title_sort | personal glucose meter based portable method for creatine kinase assay |
| topic | Personal glucose meter Creatine kinase Hexokinase Enzyme assay Statin-associated muscle symptoms |
| url | http://www.sciencedirect.com/science/article/pii/S2590137025000020 |
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