Dysregulated monocyte compartment in PACS patients
Introduction1-5% of all patients with COVID-19, a disease caused by infection with Severe Acute Respiratory Syndrome Virus 2 (SARS-Cov-2), even those with mild COVID-19 symptoms, continue to have symptoms after initial recovery. Symptoms associated with the post-acute sequelae of COVID-19 (PACS) inc...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1613034/full |
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| author | Romy Kronstein-Wiedemann Romy Kronstein-Wiedemann Madeleine Teichert Elisa Michel Janina Berg George Robinson Kristin Tausche Kristin Tausche Martin Kolditz Martin Kolditz Johannes Bergleiter Jessica Thiel Jessica Thiel Dirk Koschel Dirk Koschel Dirk Koschel Stephan R. Künzel Stephan R. Künzel Kristina Hölig Kristina Hölig Kristina Hölig Torsten Tonn Torsten Tonn Torsten Tonn Manuela Rossol Manuela Rossol |
| author_facet | Romy Kronstein-Wiedemann Romy Kronstein-Wiedemann Madeleine Teichert Elisa Michel Janina Berg George Robinson Kristin Tausche Kristin Tausche Martin Kolditz Martin Kolditz Johannes Bergleiter Jessica Thiel Jessica Thiel Dirk Koschel Dirk Koschel Dirk Koschel Stephan R. Künzel Stephan R. Künzel Kristina Hölig Kristina Hölig Kristina Hölig Torsten Tonn Torsten Tonn Torsten Tonn Manuela Rossol Manuela Rossol |
| author_sort | Romy Kronstein-Wiedemann |
| collection | DOAJ |
| description | Introduction1-5% of all patients with COVID-19, a disease caused by infection with Severe Acute Respiratory Syndrome Virus 2 (SARS-Cov-2), even those with mild COVID-19 symptoms, continue to have symptoms after initial recovery. Symptoms associated with the post-acute sequelae of COVID-19 (PACS) include, among others, fatigue, shortness of breath, cough, and cognitive dysfunction. Since the dysregulated immune response appears to be caused by the sustained activation of certain immune cells, including monocytes, and the release of specific cytokines, the aim of our study was to investigate the effect of PACS disease on monocyte subpopulations.MethodsTwenty-two healthy and thirty-two patients with PACS were included into this study. We performed blood gas analysis and measured hematological parameters from peripheral blood of PACS patients and compared them with healthy donors. Surface markers to identify monocyte subpopulations were analyzed by flow cytometry.ResultsPACS patients had higher numbers of intermediate and CD56+ monocytes, whereas the numbers of total monocytes, classical and non-classical monocytes were normal compared to healthy donors. Comparison of patients with and without fatigue, cough, and dyspnea showed no difference in monocyte subset frequencies. However, patients with cognitive dysfunction had increased numbers of non-classical monocytes compared to patients without this symptom.DiscussionThis suggests a disturbed homeostasis of the monocyte subsets in the peripheral blood of patients with PACS. |
| format | Article |
| id | doaj-art-37c7299b8e5247f4a66f8e6cfd1ef2e7 |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-37c7299b8e5247f4a66f8e6cfd1ef2e72025-08-20T02:32:33ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.16130341613034Dysregulated monocyte compartment in PACS patientsRomy Kronstein-Wiedemann0Romy Kronstein-Wiedemann1Madeleine Teichert2Elisa Michel3Janina Berg4George Robinson5Kristin Tausche6Kristin Tausche7Martin Kolditz8Martin Kolditz9Johannes Bergleiter10Jessica Thiel11Jessica Thiel12Dirk Koschel13Dirk Koschel14Dirk Koschel15Stephan R. Künzel16Stephan R. Künzel17Kristina Hölig18Kristina Hölig19Kristina Hölig20Torsten Tonn21Torsten Tonn22Torsten Tonn23Manuela Rossol24Manuela Rossol25Laboratory for Experimental Transfusion Medicine, Transfusion Medicine, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyInstitute for Transfusion Medicine, German Red Cross Blood Donation Service North-East, Dresden, GermanyInstitute for Transfusion Medicine, German Red Cross Blood Donation Service North-East, Dresden, GermanyMolecular Immunology, Faculty of Health Sciences, Brandenburgische Technische Universität (BTU) Cottbus-Senftenberg, Senftenberg, GermanyMolecular Immunology, Faculty of Health Sciences, Brandenburgische Technische Universität (BTU) Cottbus-Senftenberg, Senftenberg, GermanyLaboratory for Experimental Transfusion Medicine, Transfusion Medicine, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyDivision of Pneumology, Medical Department I, University Hospital Carl Gustav Carus and Technische Universität (TU) Dresden, Dresden, GermanyEast German Lung Center/Ostdeutsches Lungenzentrum, Dresden-Coswig, GermanyDivision of Pneumology, Medical Department I, University Hospital Carl Gustav Carus and Technische Universität (TU) Dresden, Dresden, GermanyEast German Lung Center/Ostdeutsches Lungenzentrum, Dresden-Coswig, GermanyDepartment of Transfusion Medicine, Medical Clinic and Polyclinic I, University Hospital Carl Gustav Carus, Dresden, GermanyLaboratory for Experimental Transfusion Medicine, Transfusion Medicine, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyInstitute for Transfusion Medicine, German Red Cross Blood Donation Service North-East, Dresden, GermanyDivision of Pneumology, Medical Department I, University Hospital Carl Gustav Carus and Technische Universität (TU) Dresden, Dresden, GermanyEast German Lung Center/Ostdeutsches Lungenzentrum, Dresden-Coswig, GermanyDepartment of Internal Medicine and Pneumology, Fachkrankenhaus Coswig, Lung Center, Coswig, GermanyLaboratory for Experimental Transfusion Medicine, Transfusion Medicine, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyInstitute for Transfusion Medicine, German Red Cross Blood Donation Service North-East, Dresden, GermanyLaboratory for Experimental Transfusion Medicine, Transfusion Medicine, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyInstitute for Transfusion Medicine, German Red Cross Blood Donation Service North-East, Dresden, GermanyDepartment of Transfusion Medicine, Medical Clinic and Polyclinic I, University Hospital Carl Gustav Carus, Dresden, GermanyLaboratory for Experimental Transfusion Medicine, Transfusion Medicine, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, GermanyInstitute for Transfusion Medicine, German Red Cross Blood Donation Service North-East, Dresden, GermanyInstitute for Transfusion Medicine and Immunohematology, Goethe University Hospital Medical School, German Red Cross Blood Donor Service, Frankfurt, GermanyMolecular Immunology, Faculty of Health Sciences, Brandenburgische Technische Universität (BTU) Cottbus-Senftenberg, Senftenberg, GermanyFaculty of Environment and Natural Sciences, Brandenburgische Technische Universität (BTU) Cottbus-Senftenberg, Senftenberg, GermanyIntroduction1-5% of all patients with COVID-19, a disease caused by infection with Severe Acute Respiratory Syndrome Virus 2 (SARS-Cov-2), even those with mild COVID-19 symptoms, continue to have symptoms after initial recovery. Symptoms associated with the post-acute sequelae of COVID-19 (PACS) include, among others, fatigue, shortness of breath, cough, and cognitive dysfunction. Since the dysregulated immune response appears to be caused by the sustained activation of certain immune cells, including monocytes, and the release of specific cytokines, the aim of our study was to investigate the effect of PACS disease on monocyte subpopulations.MethodsTwenty-two healthy and thirty-two patients with PACS were included into this study. We performed blood gas analysis and measured hematological parameters from peripheral blood of PACS patients and compared them with healthy donors. Surface markers to identify monocyte subpopulations were analyzed by flow cytometry.ResultsPACS patients had higher numbers of intermediate and CD56+ monocytes, whereas the numbers of total monocytes, classical and non-classical monocytes were normal compared to healthy donors. Comparison of patients with and without fatigue, cough, and dyspnea showed no difference in monocyte subset frequencies. However, patients with cognitive dysfunction had increased numbers of non-classical monocytes compared to patients without this symptom.DiscussionThis suggests a disturbed homeostasis of the monocyte subsets in the peripheral blood of patients with PACS.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1613034/fullmonocytesintermediate monocytesCD56+ monocytesCOVID-19PACSSARS-CoV-2 |
| spellingShingle | Romy Kronstein-Wiedemann Romy Kronstein-Wiedemann Madeleine Teichert Elisa Michel Janina Berg George Robinson Kristin Tausche Kristin Tausche Martin Kolditz Martin Kolditz Johannes Bergleiter Jessica Thiel Jessica Thiel Dirk Koschel Dirk Koschel Dirk Koschel Stephan R. Künzel Stephan R. Künzel Kristina Hölig Kristina Hölig Kristina Hölig Torsten Tonn Torsten Tonn Torsten Tonn Manuela Rossol Manuela Rossol Dysregulated monocyte compartment in PACS patients Frontiers in Immunology monocytes intermediate monocytes CD56+ monocytes COVID-19 PACS SARS-CoV-2 |
| title | Dysregulated monocyte compartment in PACS patients |
| title_full | Dysregulated monocyte compartment in PACS patients |
| title_fullStr | Dysregulated monocyte compartment in PACS patients |
| title_full_unstemmed | Dysregulated monocyte compartment in PACS patients |
| title_short | Dysregulated monocyte compartment in PACS patients |
| title_sort | dysregulated monocyte compartment in pacs patients |
| topic | monocytes intermediate monocytes CD56+ monocytes COVID-19 PACS SARS-CoV-2 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1613034/full |
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