Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters
The approach of two different ionization techniques including electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was tested for the analysis of cholesteryl esters (CEs). The retention time (RT), sig...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/650927 |
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| author | Hae-Rim Lee Sunil Kochhar Soon-Mi Shim |
| author_facet | Hae-Rim Lee Sunil Kochhar Soon-Mi Shim |
| author_sort | Hae-Rim Lee |
| collection | DOAJ |
| description | The approach of two different ionization techniques including electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was tested for the analysis of cholesteryl esters (CEs). The retention time (RT), signal intensity, protonated ion, and product ion of CEs were compared between ESI and APCI. RT of CEs from both ionizations decreased with increasing double bonds, while it increased with longer carbon chain length. The ESI process generated strong signal intensity of precursor ions corresponding to [M+Na]+ and [M+NH4]+ regardless of the number of carbon chains and double bonds in CEs. On the other hand, the APCI process produced a protonated ion of CEs [M+H]+ with a weak signal intensity, and it is selectively sensitive to detect precursor ions of CEs with unsaturated fatty acids. The ESI technique proved to be effective in ionizing more kinds of CEs than the APCI technique. |
| format | Article |
| id | doaj-art-37ad470e4d8944b3a5fa3c9610e017d5 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-37ad470e4d8944b3a5fa3c9610e017d52025-02-03T05:58:24ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/650927650927Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl EstersHae-Rim Lee0Sunil Kochhar1Soon-Mi Shim2Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Seoul 143-747, Republic of KoreaDepartment of Lipids & Off-Flavors, Nestlé Research Center, Nestec, Vers-chez-les-Blanc, 1000 Lausanne 26, SwitzerlandDepartment of Food Science and Technology, Sejong University, 98 Gunja-dong, Seoul 143-747, Republic of KoreaThe approach of two different ionization techniques including electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was tested for the analysis of cholesteryl esters (CEs). The retention time (RT), signal intensity, protonated ion, and product ion of CEs were compared between ESI and APCI. RT of CEs from both ionizations decreased with increasing double bonds, while it increased with longer carbon chain length. The ESI process generated strong signal intensity of precursor ions corresponding to [M+Na]+ and [M+NH4]+ regardless of the number of carbon chains and double bonds in CEs. On the other hand, the APCI process produced a protonated ion of CEs [M+H]+ with a weak signal intensity, and it is selectively sensitive to detect precursor ions of CEs with unsaturated fatty acids. The ESI technique proved to be effective in ionizing more kinds of CEs than the APCI technique.http://dx.doi.org/10.1155/2015/650927 |
| spellingShingle | Hae-Rim Lee Sunil Kochhar Soon-Mi Shim Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters International Journal of Analytical Chemistry |
| title | Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters |
| title_full | Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters |
| title_fullStr | Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters |
| title_full_unstemmed | Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters |
| title_short | Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters |
| title_sort | comparison of electrospray ionization and atmospheric chemical ionization coupled with the liquid chromatography tandem mass spectrometry for the analysis of cholesteryl esters |
| url | http://dx.doi.org/10.1155/2015/650927 |
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